| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001372 |
| 产品用途: | 5' AAVLINK plasmid for KDM5C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM5C |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KDM5C-FLAG 6629 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6629)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6629
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3330
/codon_start=1
/label=KDM5C
/translation="GHLGCHARTVGRGCQCSP*PCCG*SPGRAAGTADHC*TLGGESPP
LPGGQAEASTSHT*GHNP*SGKHPCSPAQHPGSQGGSC*GPGLDC*C**DPKW*PLPLP
G*LGGPSSCGPGPTCGAGGAETARATGTDSALLEGEGLQDLPQEKFLLHAAGGSLPMCR
CRLRQHQAQPVDGEGAGVVQI*HRAAGAVCAGPQGPRLCDRGLQGGGTEGEGGYPAAAS
HQFGQAQSTGIIEHGLLYNLYLCVWAGAGWGGSSAV*PVSGLVPWAVCVSASPPQLSEA
QSHLIPTAGLVGMGHQIPVSTVYALKAPAPGDHPGTAGSPAETACAAARGRGPAVPHRE
GHQLARPRQAGSGL*RCDCSFGTAG*APPTATG*T*T*GAS*LPCSPCF*PPQRGQWQG
YA*GPGLTGEWRQCDQS*EGSPGGGLR*ERSGAAVLAVATVDWPCVGTA*GNPGPLGGA
HDGGGPARGDPG*EPQHMAAAAGWTAPRPGEDPHTSGAGEGRASRESGSGPGPGEAAAA
EGGSGWGGR*PSPRGARAKEGTELRARG*GGPGGGRAGGGDWG*GPPCTHPHHWQPQHP
GEPEWLGTGGRDHFRPLGPFLHSDSPAASALPTAAASATV"
misc_feature 1718..1723
/label=SDS
misc_feature 2102..2107
/label=SDS
misc_feature 2123..2128
/label=SDS
misc_feature 2192..2197
/label=SDS
misc_feature 2304..2309
/label=SDS
misc_feature 2620..2625
/label=SDS
misc_feature 2747..2752
/label=SDS
misc_feature 3119..3124
/label=SDS
misc_feature 3128..3133
/label=SDS
misc_feature 3131..3136
/label=SDS
misc_feature 3146..3151
/label=SDS
CDS 3337..3402
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3418..3849
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3562..3567
/label=SDS
repeat_region 3892..4032
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3892..4021
/label=AAV2 ITR
rep_origin 4107..4562
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4844..4948
/gene="bla"
/label=AmpR promoter
CDS 4949..5809
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5980..6568
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggcacctt ggctgtcatg
1501 cgaggactgt tggtcgcggg tgccagtgta gcccctagcc ctgctgtgga taaagcccag
1561 gccgagctgc aggaactgct gaccattgct gaacgctggg aggagaaagc ccacctctgc
1621 ctggaggcca ggcagaagca tccaccagcc acacttgagg ccataatccg tgaagcggaa
1681 aacatccctg ttcacctgcc caacatccag gctctcaagg aggctcttgc taaggcccgg
1741 gcctggattg ctgatgttga tgagatccaa aatggtgacc actacccctg cctggatgac
1801 ttggagggcc tagtagctgt gggccgggac ctacctgtgg ggctggagga gctgagacag
1861 ctagagctac aggtactgac agcgcactcc tggagggaga aggcctccaa gaccttcctc
1921 aagaaaaatt cttgctacac gctgctggag gttctctgcc catgtgcaga tgccggctca
1981 gacagcacca agcgcagccg gtggatggag aaggagctgg ggttgtacaa atctgacaca
2041 gagctgctgg ggctgtctgc gcaggacctc agggacccag gctctgtgat cgtggccttc
2101 aaggaggggg aacagaagga gaaggagggt atcctgcagc tgcgtcgcac caattcggcc
2161 aagcccagtc cactggcatc atcgagcacg gcctcctcta caacctctat ctgtgtgtgt
2221 gggcaggtgc tggctggggc gggagctctg cagtgtgacc tgtgtcagga ctggttccat
2281 gggcggtgtg tgtcagtgcc tcgcctcctc agctctccga ggcccaatcc cacctcatcc
2341 ccactgctgg cctggtggga atgggacacc aaattcctgt gtccactgtg tatgcgctca
2401 aggcgcccgc gcctggagac catcctggca ctgctggtag ccctgcagag actgcctgtg
2461 cggctgcccg agggcgaggc cctgcagtgc ctcacagaga gggccatcag ctggcaaggc
2521 cgcgccaggc aggctctggc ctctgaagat gtgactgctc ttttgggacg gctggctgag
2581 ctccgccaac ggctacaggc tgaacctaga cctgaggagc ctcctaacta ccctgcagcc
2641 cctgcttctg accccctcag agagggcagt ggcaaggata tgcctaaggt ccagggctta
2701 ctggagaatg gagacagtgt gaccagtcct gagaaggtag ccccggagga gggctcaggt
2761 aagagagatc tggagctgct gtcctcgctg ttgccacagt tgactggccc tgtgttggaa
2821 ctgcctgagg caacccgggc ccccttggag gagctcatga tggaggggga cctgctcgag
2881 gtgaccctgg atgagaacca cagcatatgg cagctgctgc aggctggaca gcccccagac
2941 ctggagagga tccgcacact tctggagctg gagaaggcag agcgtcacgg gagtcgggct
3001 cggggccggg ccctggagag gcggcggcgg cggaaggtgg atcggggtgg ggagggcgat
3061 gacccagccc gagaggagct agagccaaag agggtacgga gctcagggcc agaggctgag
3121 gaggtccagg aggaggaaga gctggaggag gagactgggg gtgagggccc ccctgcaccc
3181 atccccacca ctggcagccc cagcacccag gagaaccaga atggcttgga accggcggaa
3241 gggaccactt caggcccctc ggcccctttc tccactctga ctccccggct gcatctgccc
3301 tgcccacagc agccgcctca gcaacagttg tgtacagact acaaagacca tgacggtgat
3361 tataaagatc atgacatcga ctacaaggat gacgatgaca agtaagaatt ctgatgactc
3421 gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
3481 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
3541 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg
3601 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
3661 attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc
3721 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
3781 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
3841 gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
3901 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
3961 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
4021 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
4081 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
4141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
4201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4321 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
4441 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
4501 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
4561 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
4621 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
4681 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
4741 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
4801 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
4861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
4921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
4981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
5041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
5101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
5161 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
5221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
5281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
5341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
5401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
5461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
5521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
5581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
5641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
5701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
5761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
5821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
5881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
5941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
6001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
6061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
6121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
6181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
6241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
6301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
6361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
6421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
6481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
6541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
6601 ggccttttgc tggccttttg ctcacatgt
//
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