| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001369 |
| 产品用途: | 5' AAVLINK plasmid for KDM5B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM5B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG 5948 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5948)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5948
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2661
/codon_start=1
/label=KDM5B
/translation="GNGSLAVSQTRQ*REIAVASPRCGYKNLPMSEGPSCPYDSM*TLQ
GCFPHQLCGGTQYFTGPANLALSPLSEVRETSIRENSAPARLPSAYPSSPS*GRCTSIY
D*KNRELAAQSPATAFVRES*ICARSSGLRTVI*QMASLSRTGVRHKQGISTSWHNIIF
FA**LGQQNLIFALPLLNWTKLYPPPWC*SRSE*TIDGSPAAPGIPS*NSGTLPDFTCK
AKPCSAD*PKLTSETQQ*EE*LLPREARWN*QS*EKTEETPGKRGPLQ*AVGTS*ENAD
PQKEENQTEPPQGHEQFQVRERA*L*ISSFC*NSFPALRHILFRTGRL*G*RCHLPSCE
LPAARRR*GGLGPV*WQLQSVVSSGLCWCLPRDGRERRLHLCALYCEGRTKPK"
misc_feature 1948..1953
/label=SDS
CDS 2668..2733
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2737..3168
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 2881..2886
/label=SDS
repeat_region 3211..3351
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3211..3340
/label=AAV2 ITR
rep_origin 3426..3881
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4163..4267
/gene="bla"
/label=AmpR promoter
CDS 4268..5128
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5299..5887
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggaaatgga agccttgcag
1501 tctctcagac tcgccaatga agggaaattg ctgtcgcctc tccaagatgt ggatataaaa
1561 atctgcctat gtcagaaggc cccagctgcc cctatgattc aatgtgaact ctgcagggat
1621 gctttccaca ccagttgtgt ggcggtaccc agtatttcac agggcctgcg aatctggctt
1681 tgtccccatt gtcggaggtc agagaaacct ccattagaga aaattctgcc cctgctcgcc
1741 tcccttcagc gtatccgagt tcgccttcct gagggagatg cacttcgata tatgattgaa
1801 agaaccgtga actggcagca cagagcccag caactgcttt cgtcagggaa tcttaaattt
1861 gtgcaagatc gagtgggctc aggactgtta tatagcagat ggcaagcctc agcaggacag
1921 gtgtcagaca caaacaaggt atctcaacct cctggcacaa catcattttc tttgcctgat
1981 gactgggaca acagaacctc atatttgcac tcccccttct caactggacg aagttgtatc
2041 cccctccatg gtgttagtcc agaagtgaat gaactattga tggaagccca gctgctccag
2101 gtatcccttc ctgaaattca ggaactttac cagactttac ttgcaaagcc aagccctgct
2161 cagcagactg accgaagctc accagtgaga cccagcagtg agaagaatga ctgttgccga
2221 gggaagcgag atggaattaa cagtcttgag agaaaactga agagacgcct ggaaagagag
2281 ggcctctcca gtgagcggtg ggaacgagtt aagaaaatgc ggacccccaa aaagaagaaa
2341 atcaaactga gccaccccaa ggacatgaac aatttcaagt tagagagaga gcgtagctat
2401 gaattagttc gttctgctga aactcattcc ctgccctcag acacatccta ttccgaacag
2461 gaagactctg aggatgaaga tgccatctgc ccagctgtga gctgcctgca gccagaagga
2521 gatgaggtgg actgggtcca gtgtgatggc agctgcaatc agtggtttca tcaggtctgt
2581 gttggtgtct ccccagagat ggcagagaaa gaagactaca tctgtgtgcg ctgtactgtg
2641 aaggacgcac caagccgaaa gtgtacagac tacaaagacc atgacggtga ttataaagat
2701 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
2761 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
2821 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
2881 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
2941 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
3001 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
3061 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
3121 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
3181 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
3241 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
3301 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
3361 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
3421 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
3481 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
3541 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
3601 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
3661 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
3721 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
3781 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
3841 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
3901 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
3961 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
4021 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
4081 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
4141 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
4201 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
4261 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
4321 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
4381 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
4441 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
4501 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
4561 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
4621 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
4681 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
4741 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
4801 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
4861 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
4921 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
4981 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5041 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
5101 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
5161 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
5221 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
5281 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
5341 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
5401 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
5461 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
5521 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
5581 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
5641 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
5701 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
5761 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
5821 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
5881 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
5941 tcacatgt
//
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pAAVLINKv1-pCALM1-Cre-3'-KDM5B-FLAG质粒载体应用举例
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