| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001528 |
| 产品用途: | 5' AAVLINK plasmid for KDM5A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM5A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KDM5A-FLAG 6634 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6634)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6634
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3335
/codon_start=1
/label=KDM5A
/translation="VLSPRTDIGVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGL
EETRDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQ
CELCKDWFHNSCVPLPKSSSQKKGSSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQK
LPVRLPEGEALQCLTERAMSWQDRARQALATDELSSALAKLSVLSQRMVEQAAREKTEK
IISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSSSPRQTMDYDDEETDSDEDIRETY
GYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEHAYSSASKSCSQG
SSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQA
THPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKM
DKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVL
DIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMA
ENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS"
misc_feature 1530..1535
/label=SDS
misc_feature 2416..2421
/label=SDS
misc_feature 3279..3284
/label=SDS
CDS 3342..3407
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3423..3854
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3567..3572
/label=SDS
repeat_region 3897..4037
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3897..4026
/label=AAV2 ITR
rep_origin 4112..4567
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4849..4953
/gene="bla"
/label=AmpR promoter
CDS 4954..5814
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5985..6573
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgctgagc ccccggaccg
1501 acattggtgt atatgggagt ggcaaaaata ggaggaaaaa agtaaaagaa ctaatagaaa
1561 aagaaaaaga aaaggatctg gacctggagc ctctgagtga tctggaggaa ggattggagg
1621 aaaccagaga tacagccatg gtggtggcag ttttcaaaga acgggagcaa aaagagattg
1681 aagccatgca ttctctcaga gcagccaacc tagccaagat gacaatggtg gaccgcatag
1741 aagaagtaaa attttgcatt tgccgcaaga cagccagtgg gtttatgcta cagtgtgagc
1801 tctgcaaaga ctggttccat aacagctgtg ttcctcttcc taaatcaagt tcccaaaaaa
1861 aaggatccag ctggcaagct aaagaagtaa aattcctttg ccctctttgt atgcggtctc
1921 gaaggcccag gctagagact attctgtcac tcctggtatc ccttcagaag ttgcccgtac
1981 ggttgcctga aggagaggcc ctgcagtgtt tgacagaacg tgctatgagt tggcaagata
2041 gagcgcggca ggctctagcc acagatgaac tatcctctgc cctggccaaa ctatctgtgt
2101 tgagccagcg tatggtggaa caggcggctc gagaaaaaac tgaaaagatc atcagtgcag
2161 aactccaaaa agcagctgcc aatccagact tacagggaca cttacctagt ttccagcagt
2221 ctgcttttaa ccgggtggtg agcagtgtgt catcttctcc tcgacaaaca atggactatg
2281 atgatgaaga aacagactct gatgaagaca ttcgagagac atatggctac gacatgaagg
2341 acacagccag tgtgaagtcc tctagtagtc ttgaacccaa tcttttttgt gatgaagaga
2401 ttcccatcaa atccgaggag gtggtgaccc acatgtggac agcaccttca ttttgtgcag
2461 agcatgctta ttcttctgct tctaagagtt gttctcaagg ttctagcacc ccaaggaaac
2521 aacctcggaa gagccctttg gtgccccgaa gtttggaacc tccagtgctg gagttgtcac
2581 ctggagctaa ggcacaactg gaagaactta tgatggttgg agatctcctg gaagtatctc
2641 tggacgagac tcaacacata tggcggattt tgcaggccac acacccaccc tctgaagaca
2701 gattcttgca tatcatggag gatgacagca tggaagagaa accactaaaa gtgaaaggaa
2761 aggactcttc agagaagaaa cggaaacgga agctagaaaa ggtagagcaa ctttttggag
2821 aaggaaaaca gaagtccaag gagttaaaga aaatggacaa acctagaaag aagaaattaa
2881 aattaggtgc agacaaatca aaggagctga ataaactggc caagaaacta gcaaaagaag
2941 aagagagaaa gaaaaagaag gagaaggctg ctgcagccaa agttgaactt gtgaaagaga
3001 gcactgaaaa gaaaagagag aaaaaggtgc tggacatccc ctcaaagtat gactggtcag
3061 gagcagagga gtctgatgat gagaatgctg tgtgcgcagc acagaactgc caaaggccct
3121 gcaaggacaa ggtagactgg gtacaatgtg atggtggctg tgatgagtgg tttcatcaag
3181 tttgtgtggg tgtatctcca gaaatggctg aaaatgaaga ttacatctgt ataaactgtg
3241 caaagaagca ggggccagtt agcccaggtc cagcaccacc tccttccttc ataatgagct
3301 acaaactacc aatggaggat cttaaagaga ccagttgtac agactacaaa gaccatgacg
3361 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa gaattctgat
3421 gactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
3481 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
3541 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
3601 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
3661 tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3721 ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
3781 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
3841 gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga
3901 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
3961 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
4021 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
4081 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
4141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
4201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4321 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4441 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
4501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
4561 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4621 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
4681 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
4741 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
4801 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
4861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
4921 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
4981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
5041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
5101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
5161 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
5221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
5281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
5341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
5401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
5461 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
5521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
5581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
5641 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
5701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
5761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
5821 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
5941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
6001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
6061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
6121 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
6181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
6241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
6301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
6361 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
6421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
6481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6601 ttcctggcct tttgctggcc ttttgctcac atgt
//
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