| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001405 |
| 产品用途: | 3' AAVLINK plasmid for KDM3B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM3B |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KDM3B-FLAG 7110 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7110)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7110
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3823
/codon_start=1
/label=KDM3B
/translation="G*I*RPQNTSWPMLGTSSASS*CLRRRP**WWSHTRKWHGSELCV
VYGRCVMCVKQLSSTSTGFVANVDLGSALTVTGSGKAGHAVRQKRWVMKKFSPG*SVQR
DSPTNQRISCPHKLFLAQLFTILETWYMLPGASGELKQTALVSVDRTNLY*DLPSPMGC
HSFLA*TLVPLLETKLPSLAEEDRHQ*QLQSRTMFPKPTALTSDLKSL*KQTVRHQIAI
VN*KPSGLLALTRPHPPPPCTGWQI*QLRRLKKKQKKQGP*GRCSIKSLIHPLGWTRST
PLQRSLR*LQSFLTVCCWVPLPPTTKPKGLAFETSFTPGRENFLKPPWTQAYPFPRSSL
HPQQE*RARPAYPTFLTTSLPQW*KIRKPQMLQSGPAT*LIPRRK*RRW*WG*MC*IPI
LLTPGFVMGGFCVSMTPATKTIGRSSGSVGSKVSQCWFRGYIKSSSLSSGSQKPLARNL
ETRM*TW*TAGTVL*FPM*KFGISGMVSRSYANDYGQKMGSQWCSNSRTGLLGKIFET*
CQPGLKI*WRTFLCQNIPNEMAGSIWPLGYLATL*GLIWAPRCTTPMG**QQKIEELVQ
QIFT*MCLMLLM*WCMLGFPSGRVLMMKRYSRQLTREMPMR*RSRGFMMEKRSQVLYGT
SMQPRMQRRSGSCSERLEKNKAKRTPLIMTQFMTKVGTWTRPSVSDSMRSMACKAGLLY
SS*VMLFSYLLEPHTRFTIYTVA*K*QKTLYLQNM*STVSA*LRNSGISLTLIQIMRIN
CR*RTSFTMQ*KMRLAPSRLMNPNWQG"
misc_feature 1557..1562
/label=SDS
misc_feature 2032..2037
/label=SDS
misc_feature 2168..2173
/label=SDS
misc_feature 2193..2198
/label=SDS
misc_feature 2425..2430
/label=SDS
misc_feature 3005..3010
/label=SDS
CDS 3830..3895
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3899..4330
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4043..4048
/label=SDS
repeat_region 4373..4513
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4373..4502
/label=AAV2 ITR
rep_origin 4588..5043
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5325..5429
/gene="bla"
/label=AmpR promoter
CDS 5430..6290
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6461..7049
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggatagatc tagagacctc
1501 aaaatacatc ctggccaatg ttggggacca gttctgccag ctcgtaatgt ctgagaagga
1561 ggccatgatg atggtggagc cacaccagaa agtggcatgg aagcgagctg tgcgtggtgt
1621 acgggagatg tgtgatgtgt gtgaaacaac tctcttcaac atccactggg tttgtcgcaa
1681 atgtggattt ggggtctgcc ttgactgtta ccggctcagg aaaagccggc cacgcagtga
1741 gacagaagag atgggtgatg aagaagtttt ctcctggttg aagtgtgcaa agggacagtc
1801 ccacgaacca gagaatctca tgcccacaca aattattcct ggcacagctc tttacaatat
1861 tggagacatg gtacatgctg cccggggcaa gtggggaatt aaagcaaact gcccttgtat
1921 cagtcgacag aacaaatctg tattgagacc tgccgtcacc aatgggatgt cacagcttcc
1981 tagcataaac cctagtgcct cttctggaaa cgaaactacc ttctctggcg gaggaggacc
2041 ggcaccagta acaactccag agccggacca tgttcccaaa gccgacagca ctgacatcag
2101 atctgaagag cctctgaaaa cagacagttc ggcatcaaat agcaatagtg aactgaaagc
2161 catcaggcct ccttgccctg acacggcccc accctcctcc gccctgcact ggttggcaga
2221 tttagcaact cagaaggcta aagaagaaac aaaagaagca gggtccctga ggtcggtgct
2281 caataaagag tctcattcac cctttgggct ggactcgttc aactccactg caaaggtctc
2341 tccgctgact ccaaagcttt ttaacagtct gttgctgggt cccactgcct ccaacaacaa
2401 aaccgaaggg tctagccttc gagacctcct tcactccggg ccgggaaaac ttcctcaaac
2461 ccccttggac acaggcatac cctttccccc ggtcttctct acatcctcag caggagtgaa
2521 gagcaaggcc agcctaccca actttcttga ccacatcatt gcctcagtgg tagaaaataa
2581 gaaaacctca gatgcttcaa agcgggcctg caacttgact gatacccaga aggaagtgaa
2641 ggagatggtg atggggttaa atgtgctaga tccccatact tctcactcct ggctttgtga
2701 tgggaggctt ctgtgtctcc atgaccccag caacaaaaac aattggaaga tcttccggga
2761 gtgttggaag caaggtcagc cagtgctggt ttcgggggta cataaaaagc tcaagtctga
2821 gctctggaag ccagaagcct ttagccagga atttggagac caggatgtag acttggtgaa
2881 ctgcaggaac tgtgctataa tttccgatgt gaaagttcgg gatttctggg atggtttcga
2941 gatcatatgc aaacgactac ggtcagaaga tgggcagcca atggtgctca aactcaagga
3001 ctggcctcct ggggaagatt ttcgagacat gatgccaacc aggtttgaag atctgatgga
3061 gaaccttcct ctgccagaat ataccaaacg agatggcagg ctcaatctgg cctctaggct
3121 acctagctac tttgtaaggc ctgatctggg ccccaagatg tacaacgcct atgggttgat
3181 aacagcagaa gatagaagag ttggtacaac aaatcttcac ttagatgtgt ctgatgctgt
3241 taatgtgatg gtgtatgttg ggattcccat cggggagggt gctcatgatg aagaggtact
3301 caagacaatt gacgagggag atgccgatga ggtgacgaag cagaggattc atgatggaaa
3361 agagaagcca ggtgctttat ggcacatcta tgcagccaag gatgcagaga agatccggga
3421 gctgctccga aaggttggag aagaacaagg ccaagagaac ccccctgatc atgacccaat
3481 tcatgaccaa agttggtacc tggaccagac cctccgtaag cgactctatg aggagtatgg
3541 cgtgcaaggc tgggctattg tacagttcct aggtgatgct gttttcatac ctgctggagc
3601 cccacaccag gttcacaatc tatacagttg cataaaagta gcagaagact ttgtatctcc
3661 agaacatgta aagcactgtt tccgcctgac tcaggaattc aggcatctct ctaacactca
3721 tacaaatcat gaggataaac tgcaggtgaa gaacatcatt taccatgcag tgaaagatgc
3781 ggttggcacc ctcaaggctc atgaatccaa actggcaagg tcctgtacag actacaaaga
3841 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact
3901 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3961 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
4021 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
4081 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
4141 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
4201 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
4261 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
4321 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
4381 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
4441 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4501 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4561 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4801 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4861 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
4921 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
4981 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
5041 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
5101 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
5161 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
5221 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
5281 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
5341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
5401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
5461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
5701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
5761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
5821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
5881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
5941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
6001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
6061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
6121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
6181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
6241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
6301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
6361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
7021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
7081 tggccttttg ctggcctttt gctcacatgt
//
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