| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001525 |
| 产品用途: | 3' AAVLINK plasmid for KANK1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KANK1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KANK1-FLAG 6799 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6799)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6799
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3500
/codon_start=1
/label=KANK1
/translation="VHQFTNTETATLIESCTNTCLSTLDKQTSTQTVETRTVAVGEGRV
KDINSSTKTRSIGVGTLLSGHSGFDRPSAVKTKESGVGQININDNYLVGLKMRTIACGP
PQLTVGLTASRRSVGVGDDPVGESLENPQPQAPLGMMTGLDHYIERIQKLLAEQQTLLA
ENYSELAEAFGEPHSQMGSLNSQLISTLSSINSVMKSASTEELRNPDFQKTSLGKITGN
YLGYTCKCGGLQSGSPLSSQTSQPEQEVGTSEGKPISSLDAFPTQEGTLSPVNLTDDQI
AAGLYACTNNESTLKSIMKKKDGNKDSNGAKKNLQFVGINGGYETTSSDDSSSDESSSS
ESDDECDVIEYPLEEEEEEEDEDTRGMAEGHHAVNIEGLKSARVEDEMQVQECEPEKVE
IRERYELSEKMLSACNLLKNTINDPKALTSKDMRFCLNTLQHEWFRVSSQKSAIPAMVG
DYIAAFEAISPDVLRYVINLADGNGNTALHYSVSHSNFEIVKLLLDADVCNVDHQNKAG
YTPIMLAALAAVEAEKDMRIVEELFGCGDVNAKASQAGQTALMLAVSHGRIDMVKGLLA
CGADVNIQDDEGSTALMCASEHGHVEIVKLLLAQPGCNGHLEDNDGSTALSIALEAGHK
DIAVLLYAHVNFAKAQSPGTPRLGRKTSPGPTHRGSFD"
misc_feature 2548..2553
/label=SDS
misc_feature 2551..2556
/label=SDS
misc_feature 2554..2559
/label=SDS
misc_feature 2557..2562
/label=SDS
CDS 3507..3572
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3588..4019
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3732..3737
/label=SDS
repeat_region 4062..4202
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4062..4191
/label=AAV2 ITR
rep_origin 4277..4732
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5014..5118
/gene="bla"
/label=AmpR promoter
CDS 5119..5979
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6150..6738
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgcaccag ttcaccaaca
1501 ccgagacggc caccctcata gagtcctgca ccaacacttg tctaagcact ttggacaagc
1561 agaccagcac ccagactgtg gagacgcgga cagtagctgt aggagaaggc cgtgtcaagg
1621 acatcaactc ctccaccaag acgcggtcca ttggtgttgg aacgttgctt tctggccatt
1681 ctgggtttga caggccatca gctgtgaaga ccaaagagtc aggtgtgggg cagataaata
1741 ttaacgacaa ctatctggtt ggtctcaaaa tgaggactat agcttgtggg ccaccacagt
1801 tgactgtggg gctgacagcc agcagaagga gcgtgggggt tggggatgac cctgtagggg
1861 aatctctgga gaacccccag cctcaagctc cacttggaat gatgactggc ctggatcact
1921 acattgagcg tatccagaag ctgctggcag aacagcagac actgctggct gagaactaca
1981 gtgaactggc agaagctttc ggggaacctc actcacagat gggctccctc aactctcagc
2041 tcatcagcac cctgtcgtct atcaactctg tcatgaaatc tgcaagcact gaagagctga
2101 ggaaccctga cttccagaaa accagtctgg gtaaaatcac aggcaattat ttgggatata
2161 cctgtaagtg tgggggcctt cagtcaggaa gtcccttaag ctcccagaca tcccagcctg
2221 agcaagaagt ggggacctca gaaggaaagc caatcagcag cctggatgcc ttccccactc
2281 aggaaggtac gctgtctcca gtgaacctga cagacgacca gatcgccgct ggcctctatg
2341 catgtacaaa caatgaaagt acactgaagt ccatcatgaa gaagaaagat ggtaacaaag
2401 attcaaatgg cgcaaaaaag aatcttcagt ttgttggcat taatggaggg tatgaaacaa
2461 cttcaagtga tgattccagc tcagatgaaa gctcttcttc cgagtcagat gacgagtgtg
2521 atgtcattga gtatcctctt gaagaagagg aggaggagga ggatgaagac actcggggaa
2581 tggcagaagg gcaccatgca gttaatattg aaggtttgaa gtctgccagg gtggaagatg
2641 aaatgcaggt tcaagaatgt gaacctgaga aggtggaaat cagagagagg tatgaattaa
2701 gtgaaaagat gttgtctgca tgcaacttac tgaaaaatac tataaatgac cccaaagctt
2761 tgaccagcaa agatatgagg ttctgtctga acaccctcca gcacgagtgg ttccgcgtgt
2821 ccagtcagaa gtcagccatt ccagccatgg tgggggacta catagctgct tttgaggcca
2881 tttccccaga tgtcctccgc tatgtcatca acttggcaga cggcaacggc aacacagccc
2941 tccattacag cgtgtcccac tccaacttcg agattgtgaa gctgctgtta gatgccgatg
3001 tgtgtaatgt ggatcaccag aacaaggcag gctacacccc catcatgttg gcggccctcg
3061 ccgctgtgga agcagagaag gacatgcgga ttgtggaaga actcttcggc tgtggggatg
3121 tgaatgccaa agctagtcag gcgggacaga cggccctcat gctggcggtc agtcacggac
3181 ggatagacat ggtgaagggc cttctggcct gtggggctga tgtcaacatc caggatgacg
3241 agggctccac ggccctcatg tgtgccagcg agcacgggca cgtggagatt gtcaagctgc
3301 tgctggccca gcccggctgc aacggtcacc tagaggacaa cgatggcagc actgcgctct
3361 caatcgccct ggaagcagga cacaaggaca tcgctgttct tctgtatgcc catgtcaact
3421 ttgcaaaagc ccagtctccg ggcaccccta ggcttggaag gaagacgtct cctggcccca
3481 cccaccgagg ttcatttgat tgtacagact acaaagacca tgacggtgat tataaagatc
3541 atgacatcga ctacaaggat gacgatgaca agtaagaatt ctgatgactc aagataatca
3601 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
3661 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
3721 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
3781 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
3841 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg
3901 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
3961 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
4021 gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
4081 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
4141 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
4201 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
4261 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
4321 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
4381 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
4441 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
4501 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
4561 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
4621 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
4681 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
4741 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
4801 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
4861 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
4921 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
4981 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
5041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
5101 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
5161 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
5221 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
5281 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
5341 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
5401 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
5461 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
5521 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
5581 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
5641 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
5701 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
5761 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
5821 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
5881 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
5941 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
6001 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
6061 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
6121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
6181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
6241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
6301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
6361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
6421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
6541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
6601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
6661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
6721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
6781 tggccttttg ctcacatgt
//
