| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001501 |
| 产品用途: | 3' AAVLINK plasmid for HECW2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | HECW2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG 6504 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6504)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6504
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3203
/codon_start=1
/label=HECW2
/translation="GKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSA
GEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQ
PDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYC
QSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLII
RRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYG
LFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLR
ILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPV
TEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEI
DLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLR
GSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE"
misc_feature 2891..2896
/label=SDS
CDS 3212..3277
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3293..3724
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3437..3442
/label=SDS
repeat_region 3767..3907
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3767..3896
/label=AAV2 ITR
rep_origin 3982..4437
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4719..4823
/gene="bla"
/label=AmpR promoter
CDS 4824..5684
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5855..6443
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaaggca ttctttgtgg
1501 accacaactc ccgcaccacc actttcattg atccccggct cccactccag agcagtagac
1561 ccacaagtgc gctggttcat cggcaacacc tgacaaggca acgcagccac agtgcgggtg
1621 aggtaggaga agattctcga catgcaggac caccagttct tcccaggcca tccagtacat
1681 tcaatacagt cagtaggcca cagtaccagg acatggttcc agtggcttac aatgacaaga
1741 ttgttgcatt tctacgtcaa cccaatatct ttgaaattct acaggagcgt caacctgatc
1801 ttaccaggaa tcactcactc agggagaaga tccaatttat ccgaactgaa gggactccag
1861 gattggtgcg cctttcaagc gatgcagacc ttgttatgtt gctgagctta tttgaagaag
1921 agataatgtc atatgtgcct ccacatgcct tactccaccc cagctactgt cagtccccac
1981 gtggctctcc cgtgtcatct cctcagaact cgccaggtac ccagcgtgcc aatgcccggg
2041 ctccagcccc ttacaagcgg gatttcgaag ccaaactgag gaacttttac aggaagttag
2101 agactaaagg atatggacaa ggcccaggga agttaaagtt aattatccga agagatcact
2161 tactagaaga tgcttttaat cagattatgg gctactccag aaaagacctg cagagaaata
2221 agctatatgt caccttcgtt ggggaggaag ggctggatta cagtgggcct tctagagagt
2281 ttttcttcct ggtatccaga gaactcttta acccatatta tggcttattt gaatattcag
2341 ccaatgacac atacacagta caaataagtc ctatgtctgc ttttgtagac aatcaccatg
2401 aatggttccg attcagtggt aggatccttg gtcttgcact aatacaccag tatttgttgg
2461 atgccttctt cacacggccc ttttataagg ctcttctcag aattctatgt gacctgagtg
2521 acctagaata ccttgatgaa gagttccatc agagcctgca gtggatgaaa gacaatgata
2581 tccatgacat cctagacctc acgttcactg tgaacgaaga agtatttggg cagataactg
2641 aacgagaatt aaagccaggg ggtgccaata tcccagttac agagaagaac aagaaggagt
2701 acatcgagag gatggtgaag tggaggattg agaggggtgt tgtacagcaa acagagagct
2761 tagtgcgtgg cttctatgag gtggtggatg ccaggctggt atctgttttt gatgcaagag
2821 aactggaatt ggtcatcgca ggcacagctg aaatagacct aagtgattgg agaaacaaca
2881 cagaatatag aggaggatac catgacaatc atattgtaat tcggtggttc tgggctgcag
2941 tggaaagatt caacaatgaa caacgactaa ggttgttaca gtttgttaca ggcacatcca
3001 gcattcccta tgaaggattt gcttcactcc gagggagtaa cggcccaaga agattctgtg
3061 tggagaaatg ggggaaaatc actgctcttc ccagagcgca tacatgtttt aaccgtctgg
3121 atctgcctcc ctacccatcc ttttccatgc tttatgaaaa actgttgaca gcagttgaag
3181 aaaccagtac ttttggactt gagtgtacaa ggactacaaa gaccatgacg gtgattataa
3241 agatcatgac atcgactaca aggatgacga tgacaagtaa gaattctgat gactcgagat
3301 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
3361 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
3421 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
3481 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
3541 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
3601 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
3661 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
3721 ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
3781 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
3841 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
3901 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
3961 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
4021 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
4081 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
4141 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
4201 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
4261 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
4321 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
4381 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
4441 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
4501 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
4561 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
4621 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
4681 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
4741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
4801 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
4861 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
4921 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
4981 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
5041 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
5101 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
5161 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
5221 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
5281 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
5341 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
5401 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
5461 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
5521 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
5581 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
5641 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
5701 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
5761 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
5821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
6001 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
6061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
6121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
6181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
6241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
6301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
6361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
6421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
6481 tttgctggcc ttttgctcac atgt
//
pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-HECW2-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-HA-5'-HECW2
- 下一篇：没有了
