| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001354 |
| 产品用途: | 3' AAVLINK plasmid for GRIN2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GRIN2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG 6173 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6173)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6173
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
/note="Splicing donor"
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2886
/codon_start=2
/gene="GRIN2B"
/gene_synonym="DEE27; EIEE27; GluN2B; hNR3; MRD6;
NMDAR2B;NR2B; NR3"
/product="glutamate receptor ionotropic, NMDA 2B isoform a
precursor"
/label=GRIN2B
/note="isoform a precursor is encoded by transcriptvariant
1; glutamate [NMDA] receptor subunit epsilon-2; glutamate
receptor subunit epsilon-2; N-methyl-D-aspartate receptor
subunit 3; N-methyl D-aspartate receptor subtype 2B;
GluN2B(alt_5'UTR); glutamate receptor ionotropic, NMDA 2B;
glutamate receptor, ionotropic, N-methyl D-aspartate 2B"
/db_xref="GI:167003331"
/db_xref="CCDS:CCDS8662.1"
/db_xref="GeneID:2904"
/db_xref="HGNC:HGNC:4586"
/db_xref="MIM:138252"
/protein_id="NP_000825.2"
/translation="SISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYSGHDDLIRSDVS
DISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHK
RYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHI
KHGTGDKHGVVSGVPAPWEKNLTNVEWEDRSGGNFCRSCPSKLHNYSTTVTGQNSGRQA
CIRCEACKKAGNLYDISEDNSLQELDQPAAPVAVTSNASTTKYPQSPTNSKAQKKNRNK
LRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFMDGSPYAHMFEMSAGESTFANNKSSV
PTAGHHHHNNPGGGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGAS
KARPDFRALVTNKPVVSALHGAVPARFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVY
EKLSSIESDV"
misc_feature 1517..1522
/label=SDS
misc_feature 1941..1946
/label=SDS
CDS 2893..2958
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2962..3393
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3106..3111
/label=SDS
repeat_region 3436..3576
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3436..3565
/label=AAV2 ITR
rep_origin 3651..4106
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4388..4492
/gene="bla"
/label=AmpR promoter
CDS 4493..5353
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5524..6112
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtccatcag caagaagccc
1501 ctggacatcg gcctcccctc ctccaagcac agccagctca gtgacctgta cggcaaattc
1561 tccttcaaga gcgaccgcta cagtggccac gacgacttga tccgctccga tgtctctgac
1621 atctcaaccc acaccgtcac ctatgggaac atcgagggca atgccgccaa gaggcgtaag
1681 cagcaatata aggacagcct gaagaagcgg cctgcctcgg ccaagtcccg cagggagttt
1741 gacgagatcg agctggccta ccgtcgccga ccgccccgct cccctgacca caagcgctac
1801 ttcagggaca aggaagggct acgggacttc tacctggacc agttccgaac aaaggagaac
1861 tcaccccact gggagcacgt agacctgacc gacatctaca aggagcggag tgatgacttt
1921 aagcgcgact ccgtcagcgg aggagggccc tgtaccaaca ggtctcacat caagcacggg
1981 acgggcgaca aacacggcgt ggtcagcggg gtacctgcac cttgggagaa gaacctgacc
2041 aacgtggagt gggaggaccg gtccgggggc aacttctgcc gcagctgtcc ctccaagctg
2101 cacaactact ccacgacggt gacgggtcag aactcgggca ggcaggcgtg catccggtgt
2161 gaggcttgca agaaagcagg caacctgtat gacatcagtg aggacaactc cctgcaggaa
2221 ctggaccagc cggctgcccc agtggcggtg acgtcaaacg cctccaccac taagtaccct
2281 cagagcccga ctaattccaa ggcccagaag aagaaccgga acaaactgcg ccggcagcac
2341 tcctacgaca ccttcgtgga cctgcagaag gaagaagccg ccctggcccc gcgcagcgta
2401 agcctgaaag acaagggccg attcatggat gggagcccct acgcccacat gtttgagatg
2461 tcagctggcg agagcacctt tgccaacaac aagtcctcag tgcccactgc cggacatcac
2521 caccacaaca accccggcgg cgggtacatg ctcagcaagt cgctctaccc tgaccgggtc
2581 acgcaaaacc ctttcatccc cacttttggg gacgaccagt gcttgctcca tggcagcaaa
2641 tcctacttct tcaggcagcc cacggtggcg ggggcgtcga aagccaggcc ggacttccgg
2701 gcccttgtca ccaacaagcc ggtggtctcg gcccttcatg gggccgtgcc agcccgtttc
2761 cagaaggaca tctgtatagg gaaccagtcc aacccctgtg tgcctaacaa caaaaacccc
2821 agggctttca atggctccag caatgggcat gtttatgaga aactttctag tattgagtct
2881 gatgtctgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
2941 aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa
3001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
3061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
3121 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
3181 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
3241 ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt
3301 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
3361 caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc
3421 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
3481 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
3541 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
3601 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
3661 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3721 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
3781 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
3841 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
3901 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
3961 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4021 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4081 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4141 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
4201 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
4261 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
4321 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
4381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
4441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
4501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
4561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
4621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
4681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
4741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
4801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
4861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
4921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
4981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
5161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
5221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
5281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
5341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
5401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
5461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
5521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
5581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
5641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
5701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
5761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
5821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
5881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
5941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-5'-GRIN2B
- 下一篇：没有了
