| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001447 |
| 产品用途: | 3' AAVLINK plasmid for GRIN2A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GRIN2A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG 6566 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6566)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6566
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3279
/codon_start=1
/label=GRIN2A
/translation="GHLQLHSWSAH*RKEEVSRLQSDGIPEQHVKTPPVSQKHFQHVQH
ELLKNGLTQKSC*LHPKRFPHHGHGFR*GEFDVLRQQVLSGEREHFWRQHERTPNICGQ
PAEG*PQ*LCIPGTTSSYSQ*VQP*HGGGGREHRIQSEL*TPAAVEEIRGFHTPGFTIP
ESSLPEG*GNSRE*DPLPKEP*VSSRRDGPL*HFRNVKSGHVPQGT*QQ*EPQNQGQL*
KVSGLQIPQGL**GRAHLPENQIKLP*RQDLHYRW*EGAWFPLRSTPVC*KCDPARERG
LPGPLPGSQ*KLPQGGLHAANEPEPLA**RGAFQQRPV*TLLQALHLERQGFPAQ*DQR
AIPAELHALQKLPFQHAHLFRPLHHEVPLQVRCLPADGEPL*HR*RPDASGDR*PSHRG
AGLPAGLGTEQCPSITKEQAKD*PSAFLR*HCRQT*GARP*QALPEHKPQGQGTASGGK
FLRQPV*CPLKQTLGEKKLPFPPRSGGQQEEQVSLARPHLR*PFPPLPQG*PTLGYWEM
PLGPLQTLVAIPGGE*QLSSVVLEVNGIVLFQGQSGPQ*CVYFGACYALCCK*E*YVLY
PQGFKFLQQ*TRVQENA*YRI*C"
CDS 3286..3351
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3355..3786
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3499..3504
/label=SDS
repeat_region 3829..3969
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3829..3958
/label=AAV2 ITR
rep_origin 4044..4499
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4781..4885
/gene="bla"
/label=AmpR promoter
CDS 4886..5746
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5917..6505
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggcatcta cagctgcatt
1501 catggagtgc acattgaaga aaagaagaag tctccagact tcaatctgac gggatcccag
1561 agcaacatgt taaaactcct ccggtcagcc aaaaacattt ccagcatgtc caacatgaac
1621 tcctcaagaa tggactcacc caaaagagct gctgacttca tccaaagagg ttccctcatc
1681 atggacatgg tttcagataa ggggaatttg atgtactcag acaacaggtc ctttcagggg
1741 aaagagagca tttttggaga caacatgaac gaactccaaa catttgtggc caaccggcag
1801 aaggataacc tcaataacta tgtattccag ggacaacatc ctcttactct caatgagtcc
1861 aaccctaaca cggtggaggt ggccgtgagc acagaatcca aagcgaactc tagaccccgg
1921 cagctgtgga agaaatccgt ggattccata cgccaggatt cactatccca gaatccagtc
1981 tcccagaggg atgaggcaac agcagagaat aggacccact ccctaaagag ccctaggtat
2041 cttccagaag agatggccca ctctgacatt tcagaaacgt caaatcgggc cacgtgccac
2101 agggaacctg acaacagtaa gaaccacaaa accaaggaca actttaaaag gtcagtggcc
2161 tccaaatacc ccaaggactg tagtgaggtc gagcgcacct acctgaaaac caaatcaagc
2221 tcccctagag acaagatcta cactatagat ggtgagaagg agcctggttt ccacttagat
2281 ccaccccagt ttgttgaaaa tgtgaccctg cccgagaacg tggacttccc ggacccctac
2341 caggatccca gtgaaaactt ccgcaagggg gactccacgc tgccaatgaa ccggaacccc
2401 ttgcataatg aagaggggct ttccaacaac gaccagtata aactctactc caagcacttc
2461 accttgaaag acaagggttc cccgcacagt gagaccagcg agcgataccg gcagaactcc
2521 acgcactgca gaagctgcct ttccaacatg cccacctatt caggccactt caccatgagg
2581 tcccccttca agtgcgatgc ctgcctgcgg atggggaacc tctatgacat cgatgaagac
2641 cagatgcttc aggagacagg taacccagcc accggggagc aggtctacca gcaggactgg
2701 gcacagaaca atgcccttca attacaaaag aacaagctaa ggattagccg tcagcattcc
2761 tacgataaca ttgtcgacaa acctagggag ctagacctta gcaggccctc ccggagcata
2821 agcctcaagg acagggaacg gcttctggag ggaaattttt acggcagcct gtttagtgtc
2881 ccctcaagca aactctcggg gaaaaaaagc tcccttttcc cccaaggtct ggaggacagc
2941 aagaggagca agtctctctt gccagaccac acctccgata accctttcct ccactcccac
3001 agggatgacc aacgcttggt tattgggaga tgcccctcgg acccttacaa acactcgttg
3061 ccatcccagg cggtgaatga cagctatctt cggtcgtcct tgaggtcaac ggcatcgtac
3121 tgttccaggg acagtcgggg ccacaatgat gtgtatattt cggagcatgt tatgccttat
3181 gctgcaaata agaataatat gtactctacc cccagggttt taaattcctg cagcaataga
3241 cgcgtgtaca agaaaatgcc tagtatcgaa tctgatgttt gtacagacta caaagaccat
3301 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtaactcgag
3361 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
3481 gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac
3541 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
3601 ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt
3661 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
3721 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
3781 ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
3841 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
3901 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
3961 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
4021 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
4081 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
4141 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
4201 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
4261 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
4321 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
4381 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
4441 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
4501 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
4561 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
4621 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
4681 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
4741 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
4801 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
4861 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
4921 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
4981 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5041 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5101 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
5161 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5221 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
5281 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
5341 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
5401 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
5461 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
5521 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
5581 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
5641 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
5701 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
5761 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
5821 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
5881 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
5941 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6001 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6061 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6121 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6181 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6241 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
6301 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
6361 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
6421 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
6481 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
6541 cttttgctgg ccttttgctc acatgt
//
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