| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001378 |
| 产品用途: | 3' AAVLINK plasmid for DIP2A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DIP2A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-DIP2A-FLAG 6723 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6723)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6723
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3436
/codon_start=1
/label=DIP2A
/translation="GRCTRVMC*CALTPVLPTSPNLVRNNQRLDQPQ*SWGTWLLGRES
LRLPGESSPTWRTATRHGSSCSWLTCCSGVPTPLLTTRCSCC*TPRAPSQALQPVSSCT
KGLREWPRL*WRRED*VLGTMWLWSTHQGWTSLPRSMAACTVAACLSPCGPRTLRTSAP
HCPPSR*SWRSASLHASSPRRLSHGCSGPRRLLLPWTSGPGPPS*TQMTSQKRR*QAFS
GPPPPMSSHTWTSACQPLGY*RE*RCRTRPQAPYAAP*SCSVSCTPRGRSPSASTPTVA
LVLPCGVCAVSTRDTNQCWCPRWSWRATCPCGCRPSASTRPASPSAPTL*WRCAPRA*A
HRRVSSG*RG*TCHVCARAWWSPRSGPGLR*PSPSPSSSRTWACRPAP*APRSGAGSTW
PSASRAQLARTPQPSTWTCGHCAMTGFVW*NGVLRTACH*WSLERSSPA*RSSSHTPRP
KDPWETHTWERSG*AAPTMPPGTTPFTGRRRFMPTTSVPG*VLETHRPSGQGPATLASF
GEQSSLMPVEGGTMHCMWLGLWMKLWSSEACGTTPSTLRPLSSEHTGASLSVPYSPGPT
CWWWWWSWMG*SRMPWTWWPW*PTWCWRSTTWSWEWWSSWTQG*SLSTLGVRSSACTCG
TASWLTSWTPSMSPTT"
misc_feature 2058..2063
/label=SDS
misc_feature 2940..2945
/label=SDS
CDS 3443..3508
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3512..3943
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3656..3661
/label=SDS
repeat_region 3986..4126
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3986..4115
/label=AAV2 ITR
rep_origin 4201..4656
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4938..5042
/gene="bla"
/label=AmpR promoter
CDS 5043..5903
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6074..6662
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggacgctgc acccgtgtaa
1501 tgtgctgatg tgccctcaca cctgtgttac caacctcccc aaacctcgtc agaaacaacc
1561 agaggttgga ccagcctcaa tgatcgtggg gaacctggtt gctgggaaga gaatcgctca
1621 ggcttccggg agagagctcg cccacctgga ggacagcgac caggcacgga agttcctgtt
1681 cctggctgac gtgctgcagt ggcgtgccca caccactcct gaccacccgc tgttcttgct
1741 gctgaacgcc aagggcaccg tcacaagcac tgcaacctgt gtccagctgc acaaaagggc
1801 tgagagagtg gccgcggctc tgatggagaa gggaagactg agtgttgggg accatgtggc
1861 tctggtctac ccaccagggg tggacctcat tgccgcgttc tatggctgct tgtactgtgg
1921 ctgcgtgcct gtcaccgtgc ggcccccgca ccctcagaac ctcggcacca cactgcccac
1981 cgtcaagatg atcgtggagg tcagcaagtc tgcatgcgtc ctcaccacgc aggctgtcac
2041 acggctgctc aggtccaagg aggctgctgc tgccgtggac atcaggacct ggcccaccat
2101 cctagacaca gatgacatcc caaaaaagaa gatagcaagc gttttcaggc ccccctcccc
2161 cgatgtcctc gcatacttgg acttcagcgt gtcaaccact gggatattag cgggagtgaa
2221 gatgtcgcac gcggccacaa gcgccttatg ccgctccata aagctgcagt gtgagctgta
2281 cccctcgcgg cagatcgcca tctgcctcga cccctactgt ggccttggtt ttgccctgtg
2341 gtgtctgtgc agtgtctact cgggacacca atcagtgctg gtgcccccgc tggagctgga
2401 gagcaacgtg tccctgtggc tgtcggccgt cagccagtac aaggcccgcg tcaccttctg
2461 ctcctactct gtgatggaga tgtgcaccaa gggcctaggc gcacagacgg gtgtcctcag
2521 gatgaagggg gtgaacctgt catgtgtgcg cacgtgcatg gtggtcgccg aggagcggcc
2581 caggattgcg ctgacccagt ccttctccaa gctcttcaag gacctgggcc tgccggcccg
2641 cgccgtaagc accacgttcg ggtgcagggt caacgtggcc atctgcctcc agggcacagc
2701 tggcccggac cccacaaccg tctacgtgga catgcgggca ctgcgccatg acagggttcg
2761 tttggtagaa cggggttctc cgcacagcct gccattgatg gagtctggaa agatcctccc
2821 cggcgtgaag gtcatcatcg cacacaccga gaccaaagga cccttgggag actcacacct
2881 gggagagatc tgggtaagca gcccccacaa tgccaccggg tactacaccg tttacgggga
2941 ggaggcgctt catgccgacc acttcagtgc ccggctgagt tttggagaca cacagaccat
3001 ctgggcaagg accggctacc ttggcttcct tcggcgaaca gagctcactg atgccagtgg
3061 agggcggcac gatgcactgt atgtggttgg gtctctggat gaaactctgg agctcagagg
3121 catgcggtac caccccatcg acattgagac ctctgtcatc cgagcacaca ggagcatcgc
3181 tgagtgtgcc gtattcacct ggaccaacct gctggtggtg gtggtggagc tggatgggct
3241 agagcaggat gccctggacc tggtggccct ggtgaccaac gtggtgctgg aggagcacta
3301 cctggtcgtg ggagtggtgg tcatcgtgga cccaggggtg atccctatca actctcgggg
3361 tgagaagcag cgcatgcacc tgcgggacgg cttcctggct gaccagctgg accccatcta
3421 tgtcgcctac aacatgtgta cagactacaa agaccatgac ggtgattata aagatcatga
3481 catcgactac aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa
3541 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
3601 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
3661 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
3721 tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt
3781 tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat
3841 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
3901 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg
3961 tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
4021 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
4081 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
4141 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
4201 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4261 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4321 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4381 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
4441 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4501 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
4561 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
4621 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
4681 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
4741 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
4801 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
4861 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
4921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
4981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
5041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
5101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
5161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
5221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
5281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
5341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
5401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
5461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
5521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
5581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
5641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
5701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
5761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
5821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
5881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
5941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
6001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
6061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
6121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
6181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
6241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
6301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
6361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
6421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
6481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
6541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
6601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
6661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
6721 tgt
//
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