| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001492 |
| 产品用途: | 3' AAVLINK plasmid for CUX1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CUX1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-CUX1-FLAG 7319 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7319)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7319
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..126
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..126
/label=AAV2 ITR
promoter 141..261
/label=pCALM1
CDS 273..1304
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1305..1345
/label=Splicing Donor
protein_bind 1352..1385
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1386..1477
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1478..4032
/codon_start=1
/label=CUX1
/translation="VTSPPGSEPRRLALMKPSSPS*SKPRGSSKCRKLQSRPSLPPHPA
AGTLMTPSAPSCSKPAGRWRPSRLPSTLP*SRHHCPRVTSPSSPPSFCPPRPCPPCPAT
HLSPSP*RSPPQLLRPVPLLCRTPRPSKRRPRTPPGWTPREQPIVHKGS*DR*KMRWAA
AVPGRTTGGARCSRREEMPPPPRRPRPKKRAAGKRRAAVAAEVAASLGPSAVSSRDPRR
QSTGRSGPALSPHTPRAQS*V*PGPAAARHHRTAPCHPPRSCPCPSPPSPRSPR*PPSS
TRSTCTRRWTPSSSPGRLRKSWPRTASARESSGRRCWACPRAASATCCPDRSHGAS*RR
KAENPSSGCSSG*TAS*ARVFYPSRASSKGQSSTP*HRSRTRCSRAV*AQKALQRPPPA
AALPLSPR*VPVSR*RA*PSWSSSPVPPSRRARTASHQSPVTRQHPTPSPQPRCLSPDT
RPSASKN**PCPRSWTPTA*PSG*RRC*RTTTSASAYLGRPS*GSPKALSLTSLPAPNP
GISSV*KDESPSSGCSCG*TTPTMWRS*WT*NGWRRKPT*SGGTAQSVTASPANRPLSA
PSTARAPAPSPSTS*RNPGWCWLRRRRRR*NERISKSHTRHQKPSKTSPPSST*KPAPS
STGSTTTGLGSAENCSLRKFRPGVRARRAPATHPRPAAAGRRPARRATAATAWRPLRAQ
AAPTPRSPSLRERPSGRRCRGRRSRRSRRPRGPRARTTPATTTTREAPWKARGPCPAPP
PRPPPPRPRPPRTPLPQPPPRRGRAPRPRAPRRRPATAAAAAPPAGPARCRAFSASPRP
RAPGTRATTPCARRRPRT*TASSTAWRRPPAGRNLSNGS"
misc_feature 1874..1879
/label=SDS
misc_feature 2024..2029
/label=SDS
misc_feature 2033..2038
/label=SDS
misc_feature 2339..2344
/label=SDS
misc_feature 2924..2929
/label=SDS
misc_feature 3006..3011
/label=SDS
misc_feature 3284..3289
/label=SDS
misc_feature 3608..3613
/label=SDS
CDS 4039..4104
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4108..4539
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4252..4257
/label=SDS
repeat_region 4582..4722
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4582..4711
/label=AAV2 ITR
rep_origin 4797..5252
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5534..5638
/gene="bla"
/label=AmpR promoter
CDS 5639..6499
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6670..7258
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgcgca gagagggagt ggccaactcc atcactaggg
121 gttcctgcgg ccgcacgcgt cgctctgcgc gaggcggctc cgccgcggcg gagggatacg
181 gcgcaccata tatatatcgc ggggcgcaga ctcgcgctcc ggcagtggtg ctgggagtgt
241 cgtggacgcc gtgccgttac tcgtacgcca ccatgtccaa tttactgacc gtacaccaaa
301 atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
361 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
421 ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag
481 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa accatccagc
541 aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca
601 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
661 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
721 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca
781 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
841 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
901 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
961 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
1021 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
1081 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
1141 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
1201 tgcaagctgg tggctggacc aatgtaaata ttgtaatgaa ctatatccgt aacctggata
1261 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttaggtaagt atcaaggtta
1321 caagacaggt ttaaggagac caataactag tataacttcg tataggatac tttatagcaa
1381 tttatgaaac tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt
1441 ggtcttactg acatccactt tgcctttctc tccacaggta acatcaccac ccggatccga
1501 gcctcggaga ctggctctga tgaagccatc aagtccatcc tagagcaagc caagagggag
1561 ctccaagtgc agaaaactgc agagccggcc cagccttcct ccgcatccgg cagcgggaac
1621 tctgatgacg ccatccgctc catcctgcag caagcccgcc gggagatgga ggcccagcag
1681 gctgccctcg accctgcctt aaagcaggca ccactgtccc agagtgacat caccatcctc
1741 acccccaagc ttctgtccac ctcgcccatg cccaccgtgt ccagctaccc acctctcgcc
1801 atctccctga agaagccctc cgcagctcct gaggccggtg cctctgctct gccgaacccc
1861 ccggccctca aaaaggaggc ccaggacgcc cccgggctgg acccccaggg agcagccgat
1921 tgtgcacaag gggtcctgag acaggtgaaa aatgaggtgg gccgcagcgg tgcctggaag
1981 gaccactggt ggagcgcggt gcagccggag agaagaaatg ccgcctcctc cgaggaggcc
2041 aaggccgaag aaacgggcgg cgggaaagag aagggcagcg gtggcagcgg aggtggcagc
2101 cagcctcggg ccgagcgcag tcagctccag ggaccctcgt cgtcagagta ctggaaggag
2161 tggcccagcg ctgagtcccc atactcccag agctcagagc tgagtctgac cggggccagc
2221 cgcagcgaga caccacagaa cagccccctg ccatcctccc cgatcgtgcc catgtccaag
2281 cccaccaagc cctcggtccc cccgctgacc cccgagcagt acgaggtcta catgtaccag
2341 gaggtggaca ccatcgagct cacccggcag gttaaggaaa agctggccaa gaacggcatc
2401 tgccagagaa tcttcgggga gaaggtgctg ggcctgtccc agggcagcgt cagcgacatg
2461 ctgtcccgac cgaagccatg gagcaagctg acgcagaaag gccgagaacc cttcatccgg
2521 atgcagctct ggctgaacgg cgagctaggc cagggtgttc tacccgtcca gggccagcag
2581 caagggccag tcctccactc cgtgacatcg ctccaggacc cgctgcagca gggctgtgtg
2641 agctcagaaa gcactccaaa gacctccgcc agctgcagcc ctgcccctga gtccccgatg
2701 agttccagtg agtcggtgaa gagcctgacc gagctggtcc agcagccctg tccccccatc
2761 gaggcgagca aggacagcaa gccaccagag cccagtgacc cgccagcatc cgactcccag
2821 cccacaaccc cgctgcctct ctccggacac tcggccctca gcatccaaga attagtagcc
2881 atgtccccgg agctggacac ctacggcata accaagcggg tgaaggaggt gctgacggac
2941 aacaacctcg gccagcgctt atttggggag accatcttag ggctcaccca aggctctgtc
3001 tctgacctcc ttgcccgccc caaaccctgg cataagctca gtctgaaagg acgagagccc
3061 ttcgtccgga tgcagctgtg gctgaacgac cccaacaatg tggagaagct gatggacatg
3121 aaacggatgg agaagaaagc ctacatgaag cggcggcaca gctcagtcag tgacagccag
3181 ccctgcgaac cgccctctgt cggcaccgag tacagccagg gcgccagccc ccagccccag
3241 caccagctga agaaaccccg ggtggtgctg gctccggagg agaaggaggc gctgaaacga
3301 gcgtatcagc aaaagccata cccgtcacca aaaaccatcg aagacctcgc cacccagctc
3361 aacctgaaaa ccagcaccgt catcaactgg ttccacaact acaggtctcg gatccgcaga
3421 gaactgttca ttgaggaaat tcaggccggg agtcagggcc aggcgggcgc cagcgactca
3481 ccctcggccc gcagcggccg ggcggcgccc agctcggagg gcgacagctg cgacggcgtg
3541 gaggccactg agggcccagg cagcgccgac accgaggagc ccaagtctca gggagaggcc
3601 gagcgggagg aggtgccgcg gccggcggag cagacggagc cgccgccctc ggggaccccg
3661 ggcccggacg acgcccgcga cgacgaccac gagggaggcc ccgtggaagg cccggggccc
3721 ctgcccagcc ccgcctccgc gaccgccacc gccgcgcccg cggcccccga ggacgccgct
3781 acctcagccg ccgccgcgcc gggggagggc cccgcggccc cgagctccgc gccgccgccc
3841 agcaacagca gcagcagcag cgccccccgc aggcccagct cgctgcagag ccttttcggc
3901 ctccccgagg ccgcgggcgc ccgggactcg cgcgacaacc ccctgcgcaa gaagaaggcc
3961 gcgaacttga acagcatcat ccaccgcctg gagaaggccg ccagccggga ggaacctatc
4021 gaatgggagt tctgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
4081 gactacaagg atgacgatga caagtaactc gagataatca acctctggat tacaaaattt
4141 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
4201 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
4261 ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
4321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg
4381 atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct
4441 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
4501 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc
4561 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
4621 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
4681 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
4741 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4801 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4861 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
4921 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
4981 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
5041 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
5101 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
5161 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
5221 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
5281 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
5341 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
5401 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
5461 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
5521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
5581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
5641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
5701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5881 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
6001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
7021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
