| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001540 |
| 产品用途: | 3' AAVLINK plasmid for CHD1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG 7047 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7047)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7047
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3760
/codon_start=1
/label=CHD1
/translation="VLPHQVLLLSIKKSYQPF*SLVLKNFLRNLKEKNKSPRKWI*MKS
*RELKLMKMNQVL*L*EMNCFPSSRLPTSQIWMRMTLSWNLKEIQRIGRKLFQKIKEDD
*KKKKDKRNLKKFICSQE*EIVQNRLVSMEVKGGAVEVGDTLDLIVIPSQKGKGQRNVE
DHGLFLGRILKDLVMQKLGGLSRAIRNLVVLWKD*MQLLEMLS*LISQKQTLDDWENWY
IMVALKH*RIVLQEQNEQVVDSEK*RVQHSEYQEYR*MPN*SSPMKKN*YLCTNPFLLI
QKKESSILSHATQRQLILI*TGAKKMIPIC*LASMNMDMEAGK*LKWILTSV*HTRFFQ
MIPIKNHKQNSCRPVQTTSSNYLVEILQKKKLFLVREVQRGEKQELRRIKQ*SL*K*KR
K*RVILLLCLQRSLMKMMIN*VNPSLMVGKDPRNLQCQMLQFISRQVVNQFPFLKNLKS
WIRRHSAFVKKE*GLLKQL*NNLIGLRKAFQKENN*SILDNV**KLETISQNV*KSIQI
LNKLSNGEKTCGFLYLSLLNLMQENYINYISMLLKNGRSLSKTVIKTAT*ILT*LEIQM
WKD*KRIQITMIAAGTVIPLIDT*LSTMIIIKTDIREILTKKVIPGKDPILLLVMVKTI
VIGITTSKTADITVTERNTENWMITGVEITGQIWKEV*KIDLILIIVLTQIIGYIQTTG
QVLNIRTINLPGIIGITQTGKWTTELPAVALGHH*IRDLLMAPDLHLNIQLNTKVHRSI
PGVVGK"
protein_bind 1712..1723
/label=ZF binding site
/bound_moiety="CCR5TC zinc-finger domain"
/note="target sequence for the CCR5TC zinc-finger domain
from the CCR5-224 (+) zinc-finger nuclease (Perez et al.,
2008; Gross et al., 2013)"
RBS 2617..2628
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 3767..3832
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3836..4267
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3980..3985
/label=SDS
repeat_region 4310..4450
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4310..4439
/label=AAV2 ITR
rep_origin 4525..4980
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5262..5366
/gene="bla"
/label=AmpR promoter
CDS 5367..6227
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6398..6986
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttctgccc catcaagttc
1501 tactcctttc aataaagaag agttatcagc cattttaaag tttggtgctg aagaactttt
1561 taaggaacct gaaggagaag aacaagagcc ccaggaaatg gatatagatg aaatcttgaa
1621 gagagctgaa actcatgaaa atgaaccagg tcctttaact gtaggagatg aattgctttc
1681 ccagttcaag gttgccaact tctcaaatat ggatgaggat gacattgagt tggaacctga
1741 aagaaattca aagaattggg aggaaattat tccagaagat caaagaagac gattagaaga
1801 agaagaaaga caaaaggaac ttgaagaaat ttatatgctc ccaagaatga gaaattgtgc
1861 aaaacagatt agtttcaatg gaagtgaagg gaggcgcagt agaagtagga gatactctgg
1921 atctgatagt gattccatct cagaagggaa aaggccaaag aaacgtggaa gaccacggac
1981 tattcctcgg gagaatatta aaggatttag tgatgcagaa attaggcggt ttatcaagag
2041 ctataagaaa tttggtggtc ctctggaaag attagatgca attgctcgag atgctgagtt
2101 agttgataag tcagaaacag accttagacg actgggagaa ttggtacata atggttgcat
2161 taaagcatta aaggatagtt cttcaggaac agaacgaaca ggtggtagac tcggaaaagt
2221 gaagggtcca acattccgaa tatcaggagt acaggtgaat gccaaactag tcatctccca
2281 tgaagaagaa ttaatacctt tgcacaaatc cattccttct gatccagaag aaagaaagca
2341 gtatactatc ccatgccaca caaaggcagc tcattttgat atagactggg gcaaagaaga
2401 tgattccaat ttgttaattg gcatctatga atatggatat ggaagctggg aaatgattaa
2461 aatggatcct gacctcagtc taacacacaa gattcttcca gatgatcccg ataaaaaacc
2521 acaagcaaaa cagttgcaga cccgtgcaga ctacctcatc aaattactta gtagagatct
2581 tgcaaaaaaa gaagctcttt ctggtgcggg aagttcaaag aggagaaaag caagagctaa
2641 gaagaataaa gcaatgaagt ctataaaagt gaaagaggaa ataaagagtg attcttctcc
2701 tctgccttca gagaagtctg atgaagatga tgataaattg agtgaatcca agtctgatgg
2761 tagggaaaga tccaagaaat cttcagtgtc agatgctcca gttcatatca cggcaagtgg
2821 tgaaccagtt cccatttctg aagaatctga agagctggat cagaagacat tcagcatttg
2881 taaagaaaga atgaggcctg ttaaagcagc tttgaaacaa cttgataggc ctgagaaagg
2941 cctttcagaa agagaacaac tagagcatac tagacaatgt ttaataaaaa ttggagacca
3001 tatcacagaa tgtctaaaag agtatacaaa tcctgaacaa attaagcaat ggagaaaaaa
3061 cctgtggatt tttgtatcta agtttactga atttgatgca agaaaattac ataaattata
3121 taagcatgct attaaaaaac ggcaggagtc tcagcaaaac agtgatcaaa acagcaactt
3181 gaatcctcac gtgattagaa atccagatgt ggaaagatta aaagagaata caaatcacga
3241 tgatagcagc agggacagtt attcctctga tagacactta actcagtacc atgatcatca
3301 taaagaccga catcagggag attcttacaa aaaaagtgat tccaggaaaa gaccctattc
3361 ttcttttagt aatggtaaag accatcgtga ttgggatcac tacaagcaag acagcagata
3421 ttacagtgac agagagaaac acagaaaact ggatgatcac aggagtagag atcacaggtc
3481 aaatttggaa ggaagtttaa aagatagatc tcattctgat catcgttctc actcagatca
3541 tcggttacat tcagaccacc ggtcaagttc tgaatatacg caccataaat cttccaggga
3601 ttataggtat cactcagact ggcaaatgga ccacagagct tccagcagtg gccctaggtc
3661 accactagat cagagatctc cttatggctc cagatctcca tttgaacatt cagttgaaca
3721 caaaagtaca ccggagcata cctggagtag tcggaaaaca tgtacagact acaaagacca
3781 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaactcga
3841 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
3901 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
3961 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
4021 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
4081 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
4141 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
4201 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
4261 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
4321 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
4381 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
4441 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
4501 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
4561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
4621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4741 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
4861 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
4921 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
4981 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
5041 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
5101 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
5161 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
5221 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
5281 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
5341 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
5401 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
5461 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
5521 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
5581 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
5641 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
5701 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
5761 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
5821 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
5881 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
5941 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
6001 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
6061 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
6121 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
6181 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
6241 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
6301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
6361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
6421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
6481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
6541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
6601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
6661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
6721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
6781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
6841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
6901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
6961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
7021 ccttttgctg gccttttgct cacatgt
//
