| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001549 |
| 产品用途: | 3' AAVLINK plasmid for CADPS expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CADPS |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-CADPS-FLAG 6405 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6405)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6405
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3118
/codon_start=1
/label=CADPS
/translation="VL*KLLSHSWKGF**KILLPQCHKRR*KQLSVNVWNRLR*STILG
SQSMPKSKRIKRMQKM*AG*SLLPKSLKIQYVLLN*SLKFFSKMRSTTQSHMLIKEKPL
RGGQI*WWSMRRRSCHSLQ*TWMQP*RCNLQTHGTVFHYFSC*MIFSVLTIICAMENFT
NTCKTCLPHLLLDMWI*WSPQLHNPFTGALSGSHGNQSRV*PVTYPM*TYPM*TFPKYQ
IYQLTSL*ASHKCLLFRHRHGWLLYMMRIMGQAPQKICFGNLTPFRPSFGTCTGLKKSL
ESTWNNG*S*WQVT*SNLVSKEPGLHLKLSCKKPVDQQIFESHSQYAPCLMLWLMPKLN
QQNFAAWKWAKSINTIQK*TN*LKKLLKK**HSWLQSSLLSWKECWQNYPDMTKGLCFL
LFCHLP*RQLPNMWMYLNPGWTWPTPT*LSSAILRMSCVIRSMRRCT*KGYLINGTTAP
*T*SAPG*RTGWTYSFIFIS*KH*LGW*RKPTEISDCKGSWTPP*TARPMKRSGTVSLW
RKPQHQ*VKVGDCRASA*RTAMRKTKKT"
misc_feature 1554..1559
/label=SDS
CDS 3125..3190
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3194..3625
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3338..3343
/label=SDS
repeat_region 3668..3808
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3668..3797
/label=AAV2 ITR
rep_origin 3883..4338
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4620..4724
/gene="bla"
/label=AmpR promoter
CDS 4725..5585
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5756..6344
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgctttga aagctactct
1501 ctcactcttg gaaagggttt tgatgaaaga tattgttacc ccagtgccac aagaggaggt
1561 aaaaacagtt atccgtaaat gtctggaaca ggctgcgtta gtcaactatt ctcggctctc
1621 agagtatgcc aaaatcgaag agaatcaaaa ggatgcagaa aatgtaggcc ggttaatcac
1681 tcctgccaaa aagcttgaag atacaatacg tcttgctgaa ctagtcattg aagttcttca
1741 gcaaaatgag gagcaccacg cagagccaca tgttgataaa ggagaagcct ttgcgtggtg
1801 gtcagattta atggtggagc atgcggagac gttcctgtca ctctttgcag tagacatgga
1861 tgcagcctta gaggtgcaac ctccagacac atgggacagt tttccactat ttcagctgct
1921 gaatgatttt ctccgtactg actataattt gtgcaatgga aaatttcaca aacacctgca
1981 agacctgttt gccccacttg ttgttagata tgtggatctg atggagtcct caattgcaca
2041 atccattcac aggggctttg agcgggagtc atgggaacca gtcaagagtt taaccagtaa
2101 cctacccaat gtgaacctac ccaatgtgaa ccttcccaaa gtaccaaatc taccagttaa
2161 catccctcta ggcatcccac aaatgcctac tttttcggca ccgtcatgga tggctgctat
2221 atatgatgcg gataatgggt caggcacctc agaagatctg ttttggaaac ttgacgccct
2281 tcagaccttc attcgggacc tgcactggcc tgaagaagag tttggaaagc acctggaaca
2341 acggctgaag ttgatggcaa gtgacatgat cgaatcttgt gtcaaaagaa ccaggattgc
2401 atttgaagtt aagctgcaaa aaaccagtcg atcaacagat tttcgagtcc cacagtcaat
2461 atgcaccatg tttaatgtta tggttgatgc caaagctcaa tcaacaaaac tttgcagcat
2521 ggaaatgggc caagagcatc aataccattc aaaaatagac gaactaattg aagaaactgt
2581 taaagaaatg ataacactct tggttgcaaa gttcgttact atcttggaag gagtgctggc
2641 aaaattatcc agatatgacg aagggacttt gttttcttct tttctgtcat ttaccgtgaa
2701 ggcagcttcc aaatatgtgg atgtacctaa acccgggatg gacgtggccg acgcctacgt
2761 gactttcgtc cgccattctc aggatgtcct gcgtgataag gtcaatgagg agatgtacat
2821 agaaaggtta tttgatcaat ggtacaacag ctccatgaac gtgatctgca cctggttgac
2881 ggaccggatg gacttacagc ttcatattta tcagttgaaa acactaatta ggatggtaaa
2941 gaaaacctac agagatttcc gattgcaagg ggtcctggac tccaccttaa acagcaagac
3001 ctatgaaacg atccggaacc gtctcactgt ggaggaagcc acagcatcag tgagtgaagg
3061 tgggggactg cagggcatca gcatgaagga cagcgatgag gaagacgaag aagacgattg
3121 tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
3181 cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact
3241 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
3301 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
3361 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
3421 ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta
3481 tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3541 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
3601 gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag
3661 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
3721 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
3781 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
3841 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
3901 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
3961 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
4021 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
4081 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
4141 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
4201 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
4261 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
4321 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
4381 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
4441 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
4501 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
4561 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
4621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
4681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
4741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
4801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
4861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
4921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
4981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
5041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
5101 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
5161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
5221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
5281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
5341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
5401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
5461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
5521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
5581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
5641 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
5701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
5761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
5821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
5881 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
5941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
6001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
6061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
6121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
6181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
6241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
6301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
6361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
