| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001441 |
| 产品用途: | 3' AAVLINK plasmid for CACNA1C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CACNA1C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG 7053 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7053)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7053
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 156..276
/label=pCALM1
CDS 288..1319
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1320..1360
/label=Splicing Donor
protein_bind 1367..1400
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1401..1492
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1493..3766
/codon_start=1
/label=CACNA1C
/translation="VFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLAC
MPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTR
DWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRL
VSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLD
QVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDI
GPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDGRSAFPQ
TFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRLP
RPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRCHSRESQAAMAGQEETSQDETYE
VKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRA
SFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPP
ATPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVS
LMVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEM
ESAADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRV
YVSSL"
misc_feature 1909..1914
/label=SDS
misc_feature 2379..2384
/label=SDS
misc_feature 2403..2408
/label=SDS
misc_feature 2441..2446
/label=SDS
misc_feature 2766..2771
/label=SDS
misc_feature 2835..2840
/label=SDS
misc_feature 2970..2975
/label=SDS
misc_feature 3042..3047
/label=SDS
misc_feature 3163..3168
/label=SDS
misc_feature 3726..3731
/label=SDS
CDS 3773..3838
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3842..4273
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3986..3991
/label=SDS
repeat_region 4316..4456
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4316..4445
/label=AAV2 ITR
rep_origin 4531..4986
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5268..5372
/gene="bla"
/label=AmpR promoter
CDS 5373..6233
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6404..6992
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
541 taaaaaccat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtttgg
1501 gaaaattgcc ctgaatgata ccacagagat caaccggaac aacaactttc agaccttccc
1561 ccaggccgtg ctgctcctct tcaggtgtgc caccggggag gcctggcagg acatcatgct
1621 ggcctgcatg ccaggcaaga agtgtgcccc agagtccgag cccagcaaca gcacggaggg
1681 tgaaacaccc tgtggtagca gctttgctgt cttctacttc atcagcttct acatgctctg
1741 tgccttcctg atcatcaacc tctttgtagc tgtcatcatg gacaactttg actacctgac
1801 aagggactgg tccatccttg gtccccacca cctggatgag tttaaaagaa tctgggcaga
1861 gtatgaccct gaagccaagg gtcgtatcaa acacctggat gtggtgaccc tcctccggcg
1921 gattcagccg ccactaggtt ttgggaagct gtgccctcac cgcgtggctt gcaaacgcct
1981 ggtctccatg aacatgcctc tgaacagcga cgggacagtc atgttcaatg ccaccctgtt
2041 tgccctggtc aggacggccc tgaggatcaa aacagaaggg aacctagaac aagccaatga
2101 ggagctgcgg gcgatcatca agaagatctg gaagcggacc agcatgaagc tgctggacca
2161 ggtggtgccc cctgcaggtg atgatgaggt caccgttggc aagttctacg ccacgttcct
2221 gatccaggag tacttccgga agttcaagaa gcgcaaagag cagggccttg tgggcaagcc
2281 ctcccagagg aacgcgctgt ctctgcaggc tggcttgcgc acactgcatg acatcgggcc
2341 tgagatccga cgggccatct ctggagatct caccgctgag gaggagctgg acaaggccat
2401 gaaggaggct gtgtccgctg cttctgaaga tgacatcttc aggagggccg gtggcctgtt
2461 cggcaaccac gtcagctact accaaagcga cggccggagc gccttccccc agaccttcac
2521 cactcagcgc ccgctgcaca tcaacaaggc gggcagcagc cagggcgaca ctgagtcgcc
2581 atcccacgag aagctggtgg actccacctt caccccgagc agctactcgt ccaccggctc
2641 caacgccaac atcaacaacg ccaacaacac cgccctgggt cgcctccctc gccccgccgg
2701 ctaccccagc acagtcagca ctgtggaggg ccacgggccc cccttgtccc ctgccatccg
2761 ggtgcaggag gtggcgtgga agctcagctc caacaggtgc cactcccggg agagccaggc
2821 agccatggcg ggtcaggagg agacgtctca ggatgagacc tatgaagtga agatgaacca
2881 tgacacggag gcctgcagtg agcccagcct gctctccaca gagatgctct cctaccagga
2941 tgacgaaaat cggcaactga cgctcccaga ggaggacaag agggacatcc ggcaatctcc
3001 gaagaggggt ttcctccgct ctgcctcact aggtcgaagg gcctccttcc acctggaatg
3061 tctgaagcga cagaaggacc gagggggaga catctctcag aagacagtcc tgcccttgca
3121 tctggttcat catcaggcat tggcagtggc aggcctgagc cccctcctcc agagaagcca
3181 ttcccctgcc tcattcccta ggccttttgc caccccacca gccacacctg gcagccgagg
3241 ctggccccca cagcccgtcc ccaccctgcg gcttgagggg gtcgagtcca gtgagaaact
3301 caacagcagc ttcccatcca tccactgcgg ctcctgggct gagaccaccc ccggtggcgg
3361 gggcagcagc gccgcccgga gagtccggcc cgtctccctc atggtgccca gccaggctgg
3421 ggccccaggg aggcagttcc acggcagtgc cagcagcctg gtggaagcgg tcttgatttc
3481 agaaggactg gggcagtttg ctcaagatcc caagttcatc gaggtcacca cccaggagct
3541 ggccgacgcc tgcgacatga ccatagagga gatggagagc gcggccgaca acatcctcag
3601 cgggggcgcc ccacagagcc ccaatggcgc cctcttaccc tttgtgaact gcagggacgc
3661 ggggcaggac cgagccgggg gcgaagagga cgcgggctgt gtgcgcgcgc ggggtcgacc
3721 gagtgaggag gagctccagg acagcagggt ctacgtcagc agcctgtgta cagactacaa
3781 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
3841 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
3901 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
3961 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
4021 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
4081 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
4141 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
4201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
4261 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
4321 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4381 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4441 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4501 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4741 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4861 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
4921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
4981 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5041 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5101 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5161 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5221 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5281 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5341 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5401 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5461 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5521 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5581 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5641 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5701 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5761 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
5821 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
5881 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
5941 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6001 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6061 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
6121 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6181 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6241 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6301 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6541 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
6901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
6961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
7021 tcctggcctt ttgctggcct tttgctcaca tgt
//
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