| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001552 |
| 产品用途: | 3' AAVLINK plasmid for C4B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | C4B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-C4B-FLAG 6625 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6625)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6625
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3326
/codon_start=1
/label=C4B
/translation="GGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISK
ANSFLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQ
GSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQI
RGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYT
MEVNEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKVVEEQESRVHYT
VCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLLYFDS
VPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEV
CQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETK
ITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDS
NSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV"
misc_feature 1907..1912
/label=SDS
misc_feature 2158..2163
/label=SDS
misc_feature 2448..2453
/label=SDS
misc_feature 2561..2566
/label=SDS
misc_feature 3106..3111
/label=SDS
CDS 3333..3398
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3414..3845
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3558..3563
/label=SDS
repeat_region 3888..4028
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3888..4017
/label=AAV2 ITR
rep_origin 4103..4558
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4840..4944
/gene="bla"
/label=AmpR promoter
CDS 4945..5805
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5976..6564
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggggtttg gtgggcaatg
1501 atgagactgt ggcactcaca gcctttgtga ccatcgccct tcatcatggg ctggccgtct
1561 tccaggatga gggtgcagag ccattgaagc agagagtgga agcctccatc tcaaaggcaa
1621 actcattttt gggggagaaa gcaagtgctg ggctcctggg tgcccacgca gctgccatca
1681 cggcctatgc cctgacactg accaaggccc ctgcggacct gcggggtgtt gcccacaaca
1741 acctcatggc aatggcccag gagactggag ataacctgta ctggggctca gtcactggtt
1801 ctcagagcaa tgccgtgtcg cccaccccgg ctcctcgcaa cccatccgac cccatgcccc
1861 aggccccagc cctgtggatt gaaaccacag cctacgccct gctgcacctc ctgcttcacg
1921 agggcaaagc agagatggca gaccaggctt cggcctggct cacccgtcag ggcagcttcc
1981 aagggggatt ccgcagtacc caagacacgg tgattgccct ggatgccctg tctgcctact
2041 ggattgcctc ccacaccact gaggagaggg gtctcaatgt gactctcagc tccacaggcc
2101 ggaatgggtt caagtcccac gcgctgcagc tgaacaaccg ccagattcgc ggcctggagg
2161 aggagctgca gttttccttg ggcagcaaga tcaatgtgaa ggtgggagga aacagcaaag
2221 gaaccctgaa ggtccttcgt acctacaatg tcctggacat gaagaacacg acctgccagg
2281 acctacagat agaagtgaca gtcaaaggcc acgtcgagta cacgatggaa gtaaacgagg
2341 actatgagga ctatgagtac gatgagcttc cagccaagga tgacccagat gcccctctgc
2401 agcccgtgac acccctgcag ctgtttgagg gtcggaggaa ccgccgcagg agggaggcgc
2461 ccaaggtggt ggaggagcag gagtccaggg tgcactacac cgtgtgcatc tggcggaacg
2521 gcaaggtggg gctgtctggc atggccatcg cggacgtcac cctcctgagt ggattccacg
2581 ccctgcgtgc tgacctggag aagctgacct ccctctctga ccgttacgtg agtcactttg
2641 agaccgaggg gccccacgtc ctgctgtatt ttgactcggt ccccacctcc cgggagtgcg
2701 tgggctttga ggctgtgcag gaagtgccgg tggggctggt gcagccggcc agcgcaaccc
2761 tgtacgacta ctacaacccc gagcgcagat gttctgtgtt ttacggggca ccaagtaaga
2821 gcagactctt ggccaccttg tgttctgctg aagtctgcca gtgtgctgag gggaagtgcc
2881 ctcgccagcg tcgcgccctg gagcggggtc tgcaggacga ggatggctac aggatgaagt
2941 ttgcctgcta ctacccccgt gtggagtacg gcttccaggt taaggttctc cgagaagaca
3001 gcagagctgc tttccgcctc tttgagacca agatcaccca agtcctgcac ttcaccaagg
3061 atgtcaaggc cgctgctaat cagatgcgca acttcctggt tcgagcctcc tgccgccttc
3121 gcttggaacc tgggaaagaa tatttgatca tgggtctgga tggggccacc tatgacctcg
3181 agggacaccc ccagtacctg ctggactcga atagctggat cgaggagatg ccctctgaac
3241 gcctgtgccg gagcacccgc cagcgggcag cctgtgccca gctcaacgac ttcctccagg
3301 agtatggcac tcaggggtgc caggtgtgta cagactacaa agaccatgac ggtgattata
3361 aagatcatga catcgactac aaggatgacg atgacaagta agaattctga tgactcgaga
3421 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
3481 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
3541 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
3601 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
3661 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
3721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
3781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
3841 tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt
3901 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
3961 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
4021 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4081 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4141 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
4201 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4261 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
4321 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4381 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
4441 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
4501 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
4561 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
4621 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
4681 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
4741 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
4801 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
4921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
4981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
5041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
5161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
5221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
5281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
5341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
5401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
5701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
5761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
5821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
6001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
6181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
6241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
6301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6601 ttttgctggc cttttgctca catgt
//
