| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001489 |
| 产品用途: | 5' AAVLINK plasmid for ARHGAP5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARHGAP5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ARHGAP5-FLAG 5661 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5661)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5661
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2362
/codon_start=1
/label=ARHGAP5
/translation="VVLIILQSLLTRS*MIRR*RRKPTK*KKIKSRKRKLRTSIHQHVE
IGKVITLGCPSRIWLQLRSPYHYLLRNVWNLLKIQGYVPKDSTVSAGIKLTKTIFKSSL
IKIIISI*CQWK*Q*ML*LEPLKLSLQICQIL*FHILFIQNYWKQQKSRIKQNVFMP*K
KLLRNFIL*TMMYSDT**HI*TGLVSNIKST**QQTTYPSVFGQP**DLILKIESFCLL
LRFINLLLKHSFSSVSFSFTMEKL*KRQTLWLLHHLQTQDSWWNQWCHFSCRHHCNLS*
YNHNYKRILLVL"
CDS 2369..2434
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2450..2881
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 2594..2599
/label=SDS
repeat_region 2924..3064
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2924..3053
/label=AAV2 ITR
rep_origin 3139..3594
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3876..3980
/gene="bla"
/label=AmpR promoter
CDS 3981..4841
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5012..5600
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtggtattg ataatcctgc
1501 aatcacttct gaccaggagt tagatgataa gaagatgaag aagaaaaccc acaaagtgaa
1561 agaagataaa aagcagaaaa agaaaactaa gaacttcaat ccaccaacac gtagaaattg
1621 ggaaagtaat tactttggga tgcccctcca ggatctggtt acagctgaga agcccatacc
1681 actatttgtt gagaaatgtg tggaatttat tgaagataca gggttatgta ccgaaggact
1741 ctaccgtgtc agcgggaata aaactgacca agacaatatt caaaagcagt ttgatcaaga
1801 tcataatatc aatctagtgt caatggaagt aacagtaaat gctgtagctg gagcccttaa
1861 agctttcttt gcagatctgc cagatccttt aattccatat tctcttcatc cagaactatt
1921 ggaagcagca aaaatcccgg ataaaacaga acgtcttcat gccttgaaag aaattgttaa
1981 gaaatttcat cctgtaaact atgatgtatt cagatacgtg ataacacatc taaacagggt
2041 tagtcagcaa cataaaatca acctaatgac agcagacaac ttatccatct gtttttggcc
2101 aaccttgatg agacctgatt ttgaaaatcg agagtttctg tctactacta agattcatca
2161 atctgttgtt gaaacattca ttcagcagtg tcagtttttc ttttacaatg gagaaattgt
2221 agaaacgaca aacattgtgg ctcctccacc accttcaaac ccaggacagt tggtggaacc
2281 aatggtgcca cttcagttgc cgccaccatt gcaacctcag ctgatacaac cacaattaca
2341 aacggatcct cttggtatta tatgtacaga ctacaaagac catgacggtg attataaaga
2401 tcatgacatc gactacaagg atgacgatga caagtaagaa ttctgatgac tcgagataat
2461 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
2521 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
2581 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
2641 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
2701 gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg
2761 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
2821 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
2881 aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
2941 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
3001 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
3061 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3121 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3181 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
3241 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
3301 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
3361 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3421 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
3481 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
3541 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
3601 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
3661 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
3721 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
3841 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
3901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
3961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
4021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
4081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4201 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
4561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
4621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
4681 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
4741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
4801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
4861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
4921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
4981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
5041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5641 gctggccttt tgctcacatg t
//
