| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001387 |
| 产品用途: | 3' AAVLINK plasmid for AHDC1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | AHDC1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG 6361 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6361)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6361
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3074
/codon_start=1
/label=AHDC1
/translation="GTTASATSAASAASSSSSSFQPSPENCRQFAGASQWPFRQGYGGL
DWASEAFSQLYNPSFDCHVSEPNVILDISNYTPQKVKQQTAVSETFSESSSDSTQFNQP
VGGGGFRRANSEASSSEGQSSLSSLEKLMMDWNEASSAPGYNWNQSVLFQSSSKPGRGR
RKKVDLFEASHLGFPTSASAAASGYPSKRSTGPRQPRGGRGGGACSAKKERGGAAAKAK
FIPKPQPVNPLFQDSPDLGLDYYSGDSSMSPLPSQSRAFGVGERDPCDFIGPYSMNPST
PSDGTFGQGFHCDSPSLGAPELDGKHFPPLAHPPTVFDAGLQKAYSPTCSPTLGFKEEL
RPPPTKLAACEPLKHGLQGASLGHAAAAQAHLSCRDLPLGQPHYDSPSCKGTAYWYPPG
SAARSPPYEGKVGTGLLADFLGRTEAACLSAPHLASPPATPKADKEPLEMARPPGPPRG
PAAAAAGYGCPLLSDLTLSPVPRDSLLPLQDTAYRYPGFMPQAHPGLGGGPKSGFLGPM
AEPHPEDTFTVTSL"
misc_feature 1519..1524
/label=SDS
misc_feature 1522..1527
/label=SDS
misc_feature 1525..1530
/label=SDS
misc_feature 1528..1533
/label=SDS
misc_feature 1531..1536
/label=SDS
misc_feature 1534..1539
/label=SDS
CDS 3081..3146
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3150..3581
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3294..3299
/label=SDS
repeat_region 3624..3764
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3624..3753
/label=AAV2 ITR
rep_origin 3839..4294
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4576..4680
/gene="bla"
/label=AmpR promoter
CDS 4681..5541
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5712..6300
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaccaca gcctctgcca
1501 cctctgcagc ctctgccgcc tcctcctcct cctcctcctt ccagccctcg cccgagaact
1561 gtcggcagtt tgcgggggct tctcagtggc ctttccggca gggctatgga ggcctggact
1621 gggcctcaga ggcctttagt cagctctaca atcccagttt tgactgccac gtcagcgagc
1681 ccaacgtgat cctggacatc tccaactaca caccgcagaa ggtgaagcag cagacggctg
1741 tgtcggagac cttctctgag tcatcctccg acagcaccca gttcaatcag ccggttggtg
1801 gcggggggtt tcggcgtgcc aacagcgagg cctcaagtag tgagggccag tcgagcctgt
1861 ccagcctgga gaaactgatg atggactgga acgaggcatc atctgccccc ggctacaact
1921 ggaaccagag tgtcctcttt cagagtagct ccaagccggg ccgtggacgg cggaagaagg
1981 tggacctgtt cgaggcctca catctgggct tcccgacatc cgcctctgcc gctgcctcag
2041 gctacccatc caaacggagc actgggcccc ggcagccgcg aggtggacgg ggcggtgggg
2101 cctgctcagc caagaaggag cggggtggcg cagcggccaa agccaagttc atccccaagc
2161 cacagccagt caacccactg ttccaggaca gtcctgacct cggcctggac tactatagcg
2221 gggacagcag catgtcacca ctgccctcac agtcgagggc cttcggcgtg ggagagcgag
2281 acccctgtga cttcatagga ccctactcca tgaacccgtc cacgccttcc gatggcacct
2341 ttggccaagg cttccactgc gactcgccca gcctgggtgc tcccgagctt gatggcaagc
2401 atttcccacc tctggcccac ccacccacgg tgtttgacgc cggcctgcag aaggcatact
2461 cgcccacctg ctcgcctaca ctgggcttca aggaagagct gcggccaccg cccacaaagc
2521 tggctgcctg cgagcccctc aagcatggac tccagggggc cagcctgggc cacgcagctg
2581 cagcccaggc ccacctgagc tgccgggacc tgccgctggg ccagccccac tacgattccc
2641 ccagctgcaa gggcacagcc tattggtacc ctccaggctc agctgcccgc agcccgccct
2701 atgaaggcaa ggtgggtaca gggctgctgg ctgacttcct gggcaggacg gaggccgcgt
2761 gcctcagtgc ccctcacctg gctagcccac cagccacgcc caaggccgac aaggagccac
2821 tggaaatggc ccggccccct ggcccacccc gtggccctgc tgcagccgct gctggctatg
2881 gctgcccact ccttagtgac ttgaccctgt cccccgtgcc gagggactcg ctgctgcccc
2941 tgcaggacac cgcctacagg tacccaggct ttatgcccca ggcgcatcct ggcctgggtg
3001 ggggccccaa gagcggcttc ctggggccca tggcggaacc tcaccccgag gacacattca
3061 ccgtcacatc cctgtgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
3121 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
3181 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
3241 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
3301 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
3361 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
3421 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
3481 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
3541 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg
3601 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
3661 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
3721 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
3781 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
3841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
3901 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
3961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4021 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4141 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
4201 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4261 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4321 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4381 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4441 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
4501 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
4561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
4621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
4681 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
4741 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
4801 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
4861 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
4921 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
4981 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5041 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5101 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
5161 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5221 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5281 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5341 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5401 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
5461 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
5521 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
5581 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
5641 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
5701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
5761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
5821 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
5881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
5941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6361 t
//
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