| 质粒名称: | pAAVLINK-EFS-HA-5'-CACNA1C |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001440 |
| 产品用途: | 5' AAVLINK plasmid for CACNA1C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CACNA1C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EFS-HA-5'-CACNA1C载体质粒基本信息
pAAVLINK-EFS-HA-5'-CACNA1C质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-CACNA1C载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-CACNA1C质粒载体简介
pAAVLINK-EFS-HA-5'-CACNA1C质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-CACNA1C 7400 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7400)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7400
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..356
/label=EFS core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4540
/codon_start=1
/label=CACNA1C
/translation="VNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTP
GAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALL
CLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEY
LFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANA
LGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIY
AIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKH
GITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLV
LGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEE
KPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCR
AAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYS
LGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNS
LSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQS
LLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVD
NLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADG
ESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAV
PMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNH
ILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSA
INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQ
LFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMAL
FTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIV
TFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMF
VLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNT
FDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGE
GIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQ"
CDS complement(1229..1243)
/codon_start=1
/product="enterokinase recognition and cleavage site"
/label=enterokinase site
/translation="DDDDK"
misc_feature 1302..1307
/label=SDS
misc_feature 2398..2403
/label=SDS
misc_feature 2485..2490
/label=SDS
misc_feature 2700..2705
/label=SDS
misc_feature 2703..2708
/label=SDS
misc_feature 2799..2804
/label=SDS
misc_feature 2943..2948
/label=SDS
misc_feature 3177..3182
/label=SDS
misc_feature 4492..4497
/label=SDS
intron 4541..4581
/label=Splicing Donor
misc_feature 4585..4618
/label=lox2272-JT1571
repeat_region 4663..4803
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4663..4792
/label=AAV2 ITR
rep_origin 4878..5333
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5615..5719
/gene="bla"
/label=AmpR promoter
CDS 5720..6580
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6751..7339
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc tcgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctgtcaatg agaatacgag
421 gatgtacatt ccagaggaaa accaccaagg ttccaactat gggagcccac gccccgccca
481 tgccaacatg aatgccaatg cggcagcggg gctggcccct gagcacatcc ccaccccggg
541 ggctgccctg tcgtggcagg cggccatcga cgcagcccgg caggctaagc tgatgggcag
601 cgctggcaat gcgaccatct ccacagtcag ctccacgcag cggaagcggc agcaatatgg
661 gaaacccaag aagcagggca gcaccacggc cacacgcccg ccccgagccc tgctctgcct
721 gaccctgaag aaccccatcc ggagggcctg catcagcatt gtcgaatgga aaccatttga
781 aataattatt ttactgacta tttttgccaa ttgtgtggcc ttagcgatct atattccctt
841 tccagaagat gattccaacg ccaccaattc caacctggaa cgagtggaat atctctttct
901 cataattttt acggtggaag cgtttttaaa agtaatcgcc tatggactcc tctttcaccc
961 caatgcctac ctccgcaacg gctggaacct actagatttt ataattgtgg ttgtggggct
1021 ttttagtgca attttagaac aagcaaccaa agcagatggg gcaaacgctc tcggagggaa
1081 aggggccgga tttgatgtga aggcgctgag ggccttccgc gtgctgcgcc ccctgcggct
1141 ggtgtccgga gtcccaagtc tccaggtggt cctgaattcc atcatcaagg ccatggtccc
1201 cctgctgcac atcgccctgc ttgtgctgtt tgtcatcatc atctacgcca tcatcggctt
1261 ggagctcttc atggggaaga tgcacaagac ctgctacaac caggagggca tagcagatgt
1321 tccagcagaa gatgaccctt ccccttgtgc gctggaaacg ggccacgggc ggcagtgcca
1381 gaacggcacg gtgtgcaagc ccggctggga tggtcccaag cacggcatca ccaactttga
1441 caactttgcc ttcgccatgc tcacggtgtt ccagtgcatc accatggagg gctggacgga
1501 cgtgctgtac tgggtcaatg atgccgtagg aagggactgg ccctggatct attttgttac
1561 actaatcatc atagggtcat tttttgtact taacttggtt ctcggtgtgc ttagcggaga
1621 gttttccaaa gagagggaga aggccaaggc ccggggagat ttccagaagc tgcgggagaa
1681 gcagcagcta gaagaggatc tcaaaggcta cctggattgg atcactcagg ccgaagacat
1741 cgatcctgag aatgaggacg aaggcatgga tgaggagaag ccccgaaaca tgagcatgcc
1801 caccagtgag accgagtccg tcaacaccga aaacgtggct ggaggtgaca tcgagggaga
1861 aaactgtggg gccaggctgg cccaccggat ctccaagtca aagttcagcc gctactggcg
1921 ccggtggaat cggttctgca gaaggaagtg ccgcgccgca gtcaagtcta atgtcttcta
1981 ctggctggtg attttcctgg tgttcctcaa cacgctcacc attgcctctg agcactacaa
2041 ccagcccaac tggctcacag aagtccaaga cacggcaaac aaggccctgc tggccctgtt
2101 cacggcagag atgctcctga agatgtacag cctgggcctg caggcctact tcgtgtccct
2161 cttcaaccgc tttgactgct tcgtcgtgtg tggcggcatc ctggagacca tcctggtgga
2221 gaccaagatc atgtccccac tgggcatctc cgtgctcaga tgcgtccggc tgctgaggat
2281 tttcaagatc acgaggtact ggaactcctt gagcaacctg gtggcatcct tgctgaactc
2341 tgtgcgctcc atcgcctccc tgctccttct cctcttcctc ttcatcatca tcttctccct
2401 cctggggatg cagctctttg gaggaaagtt caactttgat gagatgcaga cccggaggag
2461 cacattcgat aacttccccc agtccctcct cactgtgttt cagatcctga ccggggagga
2521 ctggaattcg gtgatgtatg atgggatcat ggcttatggc ggcccctctt ttccagggat
2581 gttagtctgt atttacttca tcatcctctt catctgtgga aactatatcc tactgaatgt
2641 gttcttggcc attgctgtgg acaacctggc tgatgctgag agcctcacat ctgcccaaaa
2701 ggaggaggaa gaggagaagg agagaaagaa gctggccagg actgccagcc cagagaagaa
2761 acaagagttg gtggagaagc cggcagtggg ggaatccaag gaggagaaga ttgagctgaa
2821 atccatcacg gctgacggag agtctccacc cgccaccaag atcaacatgg atgacctcca
2881 gcccaatgaa aatgaggata agagccccta ccccaaccca gaaactacag gagaagagga
2941 tgaggaggag ccagagatgc ctgtcggccc tcgcccacga ccactctctg agcttcacct
3001 taaggaaaag gcagtgccca tgccagaagc cagcgcgttt ttcatcttca gctctaacaa
3061 caggtttcgc ctccagtgcc accgcattgt caatgacacg atcttcacca acctgatcct
3121 cttcttcatt ctgctcagca gcatttccct ggctgctgag gacccggtcc agcacacctc
3181 cttcaggaac catattctgt tttattttga tattgttttt accaccattt tcaccattga
3241 aattgctctg aagatgactg cttatggggc tttcttgcac aagggttctt tctgccggaa
3301 ctacttcaac atcctggacc tgctggtggt cagcgtgtcc ctcatctcct ttggcatcca
3361 gtccagtgca atcaatgtcg tgaagatctt gcgagtcctg cgagtactca ggcccctgag
3421 ggccatcaac agggccaagg ggctaaagca tgtggttcag tgtgtgtttg tcgccatccg
3481 gaccatcggg aacatcgtga ttgtcaccac cctgctgcag ttcatgtttg cctgcattgg
3541 ggtccagctc ttcaagggaa agctgtacac ctgttcagac agttccaagc agacagaggc
3601 ggaatgcaag ggcaactaca tcacgtacaa agacggggag gttgaccacc ccatcatcca
3661 accccgcagc tgggagaaca gcaagtttga ctttgacaat gttctggcag ccatgatggc
3721 cctcttcacc gtctccacct tcgaagggtg gccagagctg ctgtaccgct ccatcgactc
3781 ccacacggaa gacaagggcc ccatctacaa ctaccgtgtg gagatctcca tcttcttcat
3841 catctacatc atcatcatcg ccttcttcat gatgaacatc ttcgtgggct tcgtcatcgt
3901 cacctttcag gagcaggggg agcaggagta caagaactgt gagctggaca agaaccagcg
3961 acagtgcgtg gaatacgccc tcaaggcccg gcccctgcgg aggtacatcc ccaagaacca
4021 gcaccagtac aaagtgtggt acgtggtcaa ctccacctac ttcgagtacc tgatgttcgt
4081 cctcatcctg ctcaacacca tctgcctggc catgcagcac tacggccaga gctgcctgtt
4141 caaaatcgcc atgaacatcc tcaacatgct cttcactggc ctcttcaccg tggagatgat
4201 cctgaagctc attgccttca aacccaagca ctatttctgt gatgcatgga atacatttga
4261 cgccttgatt gttgtgggta gcattgttga tatagcaatc accgaggtaa acccagctga
4321 acatacccaa tgctctccct ctatgaacgc agaggaaaac tcccgcatct ccatcacctt
4381 cttccgcctg ttccgggtca tgcgtctggt gaagctgctg agccgtgggg agggcatccg
4441 gacgctgctg tggaccttca tcaagtcctt ccaggccctg ccctatgtgg ccctcctgat
4501 cgtgatgctg ttcttcatct acgcggtgat cgggatgcag gtaagtatca aggttacaag
4561 acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta
4621 agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
4681 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
4741 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
4801 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
4861 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4921 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
4981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
5041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
5101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
5161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
5221 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
5281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
5341 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
5401 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
5461 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
5521 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
5581 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
5641 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
5701 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
5761 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
5821 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
5881 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
5941 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
6001 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
6061 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
6121 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
6181 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
6241 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
6301 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
6361 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
6421 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
6481 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
6541 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
6601 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
6661 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
6721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
6781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
6841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
6901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
6961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
7021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
7081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
7141 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
7201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
7261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
7321 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
7381 ctggcctttt gctcacatgt
//
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