| 质粒名称: | pAAVLINK-EF1a-HA-5'-ZMYM2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001452 |
| 产品用途: | 5' AAVLINK plasmid for ZMYM2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ZMYM2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-ZMYM2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ZMYM2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ZMYM2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ZMYM2质粒载体简介
pAAVLINK-EF1a-HA-5'-ZMYM2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ZMYM2 5893 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5893)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5893
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3033
/codon_start=1
/label=ZMYM2
/translation="DTSSVGGLELTDQTPVLLGSTAMATSLTNVGNSFSGPANPLVSRS
NKFQNSSVEDDDDVVFIEPVQPPPPSVPVVADQRTITFTSSKNEELQGNDSKITPSSKE
LASQKGSVSETIVIDDEEDMETNQGQEKNSSNFIERRPPETKNRTNDVDFSTSSFSRSK
VNAGMGNSGITTEPDSEIQIANVTTLETGVSSVNDGQLENTDGRDMNLMITHVTSLQNT
NLGDVSNGLQSSNFGVNIQTYTPSLTSQTKTGVGPFNPGRMNVAGDVFQNGESATHHNP
DSWISQSASFPRNQKQPGVDSLSPVASLPKQIFQPSVQQQPTKPVKVTCANCKKPLQKG
QTAYQRKGSAHLFCSTTCLSSFSHKPAPKKLCVMCKKDITTMKGTIVAQVDSSESFQEF
CSTSCLSLYEDKQNPTKGALNKSRCTICGKLTEIRHEVSFKNMTHKLCSDHCFNRYRMA
NGLIMNCCEQCGEYLPSKGAGNNVLVIDGQQKRFCCQSCVSEYKQVGSHPSFLKEVRDH
MQDSFLMQPEKYGKLTTCTGCRTQCRFFDMTQCIGPNGYMEPYCSTACMNSHKTKYAKS
QSLGIICHFCKRNSLPQYQATMPDGKLYNFCNSSCVAKFQALSMQSSPNGQFVAPSDIQ
LKCNYCKNSFCSKPEILEWENKVHQFCSKTCSDDYKKLHCIVTYCEYCQEEKTLHETVN
FSGVKRPFCSEGCKLLYKQDFARRLGLRCVTCNYCSQLCKKGATKELDGVVRDFCSEDC
CKKFQDWYYKAA"
misc_feature 1160..1165
/label=SDS
misc_feature 2271..2276
/label=SDS
intron 3034..3074
/label=Splicing Donor
misc_feature 3078..3111
/label=lox2272-JT1571
repeat_region 3156..3296
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3156..3285
/label=AAV2 ITR
rep_origin 3371..3826
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4108..4212
/gene="bla"
/label=AmpR promoter
CDS 4213..5073
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5244..5832
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgaca caagttcagt gggaggatta gaattgactg atcagactcc
781 tgttttatta gggagtacgg ccatggcaac tagtctcacg aatgtaggaa actcatttag
841 tggtccagct aatcctttag tgtctagatc taataagttt cagaactcgt cagtggaaga
901 tgatgatgat gttgttttta tcgaacctgt acaacctccc ccaccttctg taccagtggt
961 agctgatcaa agaaccataa catttacatc atcaaaaaat gaagaactac aaggaaatga
1021 ttccaaaatt actccttcct caaaagagtt ggcatctcag aagggaagtg taagtgagac
1081 aattgtcatt gatgatgaag aggacatgga aacaaatcaa gggcaagaga aaaattcctc
1141 caattttatt gaacgaagac ctcctgagac taaaaacaga accaatgatg tggatttctc
1201 cacttccagt ttttcaagaa gtaaggtaaa tgcaggaatg ggtaatagtg gtatcaccac
1261 agaaccagac tctgaaattc agattgctaa tgttacaact ttagaaacag gtgtaagctc
1321 tgtgaatgat ggccaattag aaaatactga cgggcgagat atgaacttaa tgattacaca
1381 tgtaacatca ctgcagaata ccaacttggg agatgtctct aacggactgc agtcaagtaa
1441 ttttggtgtt aatatacaaa catacacccc atctttaact tcacagacca agactggagt
1501 aggacctttt aatcctggta gaatgaatgt ggcaggagac gtttttcaga atggagaatc
1561 tgcaactcat cataatcctg attcttggat ctcccagtca gcttcatttc cccgtaatca
1621 gaaacaacca ggggtggact ctttatcacc agtggcctca cttcctaaac agattttcca
1681 gccgtctgtc caacagcagc ctactaaacc agttaaagtc acttgtgcaa actgcaaaaa
1741 acctttacag aagggccaga cagcttatca acgaaaagga tcagctcacc tcttttgttc
1801 taccacctgc ctttcttcct tctcccacaa gcctgctcca aagaaactct gtgttatgtg
1861 taaaaaagat ataactacaa tgaaaggaac cattgttgct caagtggatt caagtgagtc
1921 cttccaggaa ttctgtagta catcttgttt atctctctat gaagacaaac agaatcctac
1981 taaaggagct ctaaataaat caagatgtac aatctgtggt aaactaactg agattcgcca
2041 tgaagtcagc tttaaaaata tgactcataa gctgtgcagt gaccactgct ttaatagata
2101 tagaatggcc aatggtttaa taatgaattg ctgtgaacag tgtggagagt acttgcccag
2161 taaaggtgct ggaaataatg ttctggtgat tgatggtcaa cagaaaagat tttgctgtca
2221 aagttgtgtc agtgaataca aacaggtagg tagccatcca agcttcctga aggaggttcg
2281 agatcacatg caggactctt tcttaatgca gcctgagaaa tatggaaaac tgacaacttg
2341 tactggttgc cgaacacagt gcaggttttt tgatatgact cagtgtatag gtcctaatgg
2401 atatatggag ccatattgtt caactgcttg tatgaacagt cacaagacaa aatatgcaaa
2461 atcacaaagt ttgggaatta tttgccattt ttgtaagcga aactctttac ctcaatacca
2521 agccacaatg cctgatggaa aactgtacaa cttttgcaat tccagttgtg tggctaaatt
2581 tcaggctcta agtatgcagt catctccaaa tggccagttt gtagcgccaa gtgatattca
2641 gttgaaatgc aactactgca aaaattcctt ttgttcaaaa ccagaaatcc tggaatggga
2701 gaacaaagtg catcagttct gcagcaaaac ttgttcagat gactataaga agttgcattg
2761 catagttaca tattgcgaat actgtcaaga ggagaagact cttcatgaaa cagtaaattt
2821 ctctggcgtt aagagacctt tctgtagtga aggctgcaaa ttattataca aacaggattt
2881 tgccagacgt ttaggattga gatgtgttac ttgcaactat tgttctcagc tatgtaagaa
2941 gggagcaact aaagaactcg atggtgttgt gagagatttc tgcagtgaag attgctgtaa
3001 aaaatttcag gattggtact acaaggctgc aaggtaagta tcaaggttac aagacaggtt
3061 taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta ttaagttatg
3121 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
3181 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
3241 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
3301 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
3361 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
3421 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
3481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
3541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
3601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
3661 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
3721 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
3781 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
3841 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
3901 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
3961 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
4021 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
4081 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
4141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
4201 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
4261 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
4321 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
4381 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
4441 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
4501 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
4561 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
4621 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
4681 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
4741 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
4801 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
4861 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
4921 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
4981 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
5041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
5101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
5161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
5221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
5281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
5341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
5401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
5461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
5521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
5581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
5641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
5701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
5761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
5821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
5881 tttgctcaca tgt
//
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