| 质粒名称: | pAAVLINK-EF1a-HA-5'-UNC13A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001536 |
| 产品用途: | 5' AAVLINK plasmid for UNC13A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | UNC13A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-UNC13A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-UNC13A载体质粒基本信息
pAAVLINK-EF1a-HA-5'-UNC13A质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-UNC13A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-UNC13A质粒载体简介
pAAVLINK-EF1a-HA-5'-UNC13A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-UNC13A 6590 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6590)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6590
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3730
/codon_start=1
/label=UNC13A
/translation="MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSW
EQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQVI
MADSEICGTKDPTFHRILLDTRFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDEQ
DKPLPVPSNQCCNWNYFGWGEQHNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNAS
VHQYSVRPPPLGSRESYSDSMHSYEEFSEPQALSPTGSSRYASSGELSQGSSQLSEDFD
PDEHSLQGSDMEDERDRDSYHSCHSSVSYHKDSPRWDQDEEELEEDLEDFLEEEELPED
EEELEEEEEEVPDDLGSYAQREDVAVAEPKDFKRISLPPAAPGKEDKAPVAPTEAPDMA
KVAPKPATPDKVPAAEQIPEAEPPKDEESFRPREDEEGQEGQDSMSRAKANWLRAFNKV
RMQLQEARGEGEMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAG
ITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWG
IARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRE
RNKPEIFELIQEIFAVTKTAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKT
GSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRV
KQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKV
APYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGV
ESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGK
ERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSP
PRASQ"
misc_feature 1017..1022
/label=SDS
misc_feature 1111..1116
/label=SDS
misc_feature 1155..1160
/label=SDS
misc_feature 1722..1727
/label=SDS
misc_feature 1746..1751
/label=SDS
misc_feature 1749..1754
/label=SDS
misc_feature 1770..1775
/label=SDS
misc_feature 1782..1787
/label=SDS
misc_feature 1785..1790
/label=SDS
misc_feature 1788..1793
/label=SDS
misc_feature 1791..1796
/label=SDS
misc_feature 1794..1799
/label=SDS
misc_feature 1899..1904
/label=SDS
misc_feature 2061..2066
/label=SDS
misc_feature 2136..2141
/label=SDS
intron 3731..3771
/label=Splicing Donor
misc_feature 3775..3808
/label=lox2272-JT1571
repeat_region 3853..3993
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3853..3982
/label=AAV2 ITR
rep_origin 4068..4523
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4805..4909
/gene="bla"
/label=AmpR promoter
CDS 4910..5770
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5941..6529
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gtctctgctt tgcgttggag tcaaaaaagc
781 caagtttgat ggtgcccaag agaaattcaa cacgtacgtg accctgaaag tgcagaatgt
841 caagagcacg accatcgcgg tgcggggcag ccagcccagc tgggagcagg atttcatgtt
901 cgagattaac cgtctggatt tgggactgac ggtggaggtg tggaataagg gtctcatctg
961 ggacacaatg gtgggcactg tgtggatccc actgaggacc atccgccagt ccaatgagga
1021 gggccctgga gagtggctga cgctggactc ccaggtcatc atggcagaca gtgagatctg
1081 tggcaccaag gaccccacct tccaccgcat cctcctggac acgcgctttg agctaccctt
1141 agacattcct gaagaggagg ctcgctactg ggccaagaag ctggagcagc tcaatgctat
1201 gcgggaccag gatgaatatt ccttccaaga tgagcaagac aagcctctgc ctgtccccag
1261 caaccagtgc tgcaactgga attattttgg ctggggtgag cagcacaacg atgaccccga
1321 cagtgcagtg gatgatcgtg acagtgacta ccgcagtgaa acgagcaaca gcatcccgcc
1381 gccctattat actacgtcac aacccaacgc ctcagtccac caatattctg ttcgcccacc
1441 acccctgggc tcccgggagt cctacagtga ctccatgcac agttacgagg agttctctga
1501 gccacaagcc ctcagcccca cgggtagcag ccgctatgcc tcttccgggg agctgagcca
1561 gggaagctct cagctgagcg aggacttcga ccctgacgag cacagcctgc agggctccga
1621 catggaggat gagcgggacc gggactccta ccactcctgc cacagctcgg tcagctacca
1681 caaagactcg cctcgctggg accaggatga ggaagagctg gaggaggacc tggaggactt
1741 cctggaggag gaggagctgc ctgaagatga ggaggagctg gaggaggagg aggaggaggt
1801 gcctgacgat ttgggcagct atgcccagcg tgaagacgta gctgtggctg agcccaaaga
1861 cttcaaacgc atcagcctcc cgccagctgc cccagggaag gaggacaagg ccccagtggc
1921 acccaccgag gcccccgaca tggccaaggt ggcccccaag ccagccacgc ccgacaaggt
1981 gcctgcagct gagcagatcc ctgaggctga gccacccaag gacgaggaga gtttcaggcc
2041 gagagaggat gaggaaggcc aggaggggca ggactccatg tccagggcca aggccaactg
2101 gctgcgtgcc ttcaacaagg tgcggatgca gctgcaggag gcccggggag aaggagagat
2161 gtctaaatcc ctatggttca aaggcggccc agggggcggt ctcatcatca tcgacagcat
2221 gccagacatc cgcaagagga aacctatccc actcgtgagc gacttggcca tgtccctggt
2281 ccagtccagg aaagcgggca tcacctcggc cttggcctcc agcacgttga acaacgagga
2341 gctgaaaaac cacgtttaca agaagaccct gcaagcctta atctacccca tctcgtgcac
2401 gacgccacac aacttcgaag tgtggacggc caccacgccc acctactgct acgagtgcga
2461 ggggctgctg tggggcatcg cgaggcaggg catgcgctgc accgagtgcg gtgtcaagtg
2521 ccacgagaag tgccaggacc tgctcaacgc cgactgcctg cagcgggctg cggagaagag
2581 ctccaagcac ggggcggagg accggacaca gaacatcatc atggtgctca aggaccgcat
2641 gaagatccgg gagcgcaaca agcccgagat cttcgagctc atccaggaga tcttcgcggt
2701 gaccaagacg gcgcacacgc agcagatgaa ggcggtcaag cagagcgtgc tggacggcac
2761 gtccaagtgg tccgccaaga tcagcatcac cgtggtctgc gcccagggct tgcaggcaaa
2821 ggacaagaca ggatccagtg acccctatgt caccgtccag gtcgggaaga ccaagaaacg
2881 gacaaaaacc atctatggga acctcaaccc ggtgtgggag gagaatttcc actttgaatg
2941 tcacaattcc tccgaccgca tcaaggtgcg cgtctgggac gaggatgacg acatcaaatc
3001 ccgcgtgaaa cagaggttca agagggaatc tgacgatttc ctggggcaga cgatcattga
3061 ggtgcggacg ctcagcggcg agatggacgt gtggtacaac ctggacaagc gaactgacaa
3121 atctgccgtg tcgggtgcca tccggctcca catcagtgtg gagatcaaag gcgaggagaa
3181 ggtggccccg taccatgtcc agtacacctg tctgcatgag aacctgttcc acttcgtgac
3241 cgacgtgcag aacaatgggg tcgtgaagat cccagatgcc aagggtgacg atgcctggaa
3301 ggtttactac gatgagacag cccaggagat tgtggacgag tttgccatgc gctacggcgt
3361 cgagtccatc taccaagcca tgacccactt tgcctgcctc tcctccaagt atatgtgccc
3421 aggggtgcct gccgtcatga gcaccctgct cgccaacatc aatgcctact acgcacacac
3481 caccgcctcc accaacgtgt ctgcctccga ccgcttcgcc gcctccaact ttgggaaaga
3541 gcgcttcgtg aaactcctgg accagctgca taactccctg cggattgacc tctccatgta
3601 ccggaataac ttcccagcca gcagcccgga gagactccag gacctcaaat ccactgtgga
3661 ccttctcacc agcatcacct tctttcggat gaaggtacaa gaactccaga gcccgccccg
3721 agccagccag gtaagtatca aggttacaag acaggtttaa ggagaccaat aagctattat
3781 tcgtatagga tactttatac gaagttatta agttatggag accggtgtgg cacgtgcgga
3841 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
3901 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
3961 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
4021 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
4081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
4141 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
4201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
4261 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
4321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
4381 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
4441 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
4501 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
4561 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
4621 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
4681 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
4741 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
4801 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
4861 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
4921 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
4981 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
5041 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
5101 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
5161 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
5221 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
5281 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
5341 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
5401 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
5461 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
5521 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
5581 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
5641 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
5701 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
5761 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
5821 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
5881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
5941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
6001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
6061 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
6121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
6181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
6241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
6301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
6361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
6421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
6481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
6541 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
