| 质粒名称: | pAAVLINK-EF1a-HA-5'-TOP2B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001506 |
| 产品用途: | 5' AAVLINK plasmid for TOP2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TOP2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-TOP2B载体质粒基本信息
pAAVLINK-EF1a-HA-5'-TOP2B质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-TOP2B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-TOP2B质粒载体简介
pAAVLINK-EF1a-HA-5'-TOP2B质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-TOP2B 6493 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6493)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6493
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 727..753
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 760..3633
/codon_start=1
/label=TOP2B
/translation="AKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSS
KKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKI
FDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFG
QLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTS
EAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKK
LPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATT
KGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKE
NMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGI
PKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREAS
HKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINF
IHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKG
LGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQ
RRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTC
FKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGT
RLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLIN
GAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVS
GEIFVVDRNTVEITELPVRTWTQVYKEQ"
RBS 3290..3298
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 3291..3296
/label=SDS
intron 3634..3674
/label=Splicing Donor
misc_feature 3678..3711
/label=lox2272-JT1571
repeat_region 3756..3896
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3756..3885
/label=AAV2 ITR
rep_origin 3971..4426
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4708..4812
/gene="bla"
/label=AmpR promoter
CDS 4813..5673
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5844..6432
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgtacc cttatgacgt acctgactat gctggatctg ccaagtcggg tggctgcggc
781 gcgggagccg gcgtgggcgg cggcaacggg gcactgacct gggtgactct ttttgatcag
841 aacaatgctg caaaaaaaga agagtcagaa actgccaaca aaaatgattc ttcaaagaag
901 ttgtctgttg agagagtgta tcagaagaag acacaacttg aacacattct tcttcgtcct
961 gatacatata ttgggtcagt ggagccattg acgcagttca tgtgggtgta tgatgaagat
1021 gtaggaatga attgcaggga ggttaccttt gtgccaggtt tatacaagat ctttgatgaa
1081 attttggtta atgctgctga caataaacag agggataaga acatgacttg tattaaagtt
1141 tctattgatc ctgaatctaa cattataagc atttggaata atgggaaagg cattccagta
1201 gtagaacaca aggtagagaa agtttatgtt cctgctttaa tttttggaca gcttttaaca
1261 tccagtaact atgatgatga tgagaaaaaa gttacaggtg gtcgtaatgg ttatggtgca
1321 aaactttgta atattttcag tacaaagttt acagtagaaa cagcttgcaa agaatacaaa
1381 cacagtttta agcagacatg gatgaataat atgatgaaga cttctgaagc caaaattaaa
1441 cattttgatg gtgaagatta cacatgcata acattccaac cagatctgtc caaatttaag
1501 atggaaaaac ttgacaagga tattgtggcc ctcatgacta gaagggcata tgatttggct
1561 ggttcgtgta gaggggtcaa ggtcatgttt aatggaaaga aattgcctgt aaatggattt
1621 cgcagttatg tagatcttta tgtgaaagac aaattggatg aaactggggt ggccctgaaa
1681 gttattcatg agcttgcaaa tgaaagatgg gatgtttgtc tcacattgag tgaaaaagga
1741 ttccagcaaa tcagctttgt aaatagtatt gcaactacaa aaggtggacg gcacgtggat
1801 tatgtggtag atcaagttgt tggtaaactg attgaagtag ttaagaaaaa gaacaaagct
1861 ggtgtatcag tgaaaccatt tcaagtaaaa aaccatatat gggtttttat taattgcctt
1921 attgaaaatc caacttttga ttctcagact aaggaaaaca tgactctgca gcccaaaagt
1981 tttgggtcta aatgccagct gtcagaaaaa ttttttaaag cagcctctaa ttgtggcatt
2041 gtagaaagta tcctgaactg ggtgaaattt aaggctcaga ctcagctgaa taagaagtgt
2101 tcatcagtaa aatacagtaa aatcaaaggt attcccaaac tggatgatgc taatgatgct
2161 ggtggtaaac attccctgga gtgtacactg atattaacag agggagactc tgccaaatca
2221 ctggctgtgt ctggattagg tgtgattgga cgagacagat acggagtttt tccactcagg
2281 ggcaaaattc ttaatgtacg ggaagcttct cataaacaga tcatggaaaa tgctgaaata
2341 aataatatta ttaaaatagt tggtctacaa tataagaaaa gttacgatga tgcagaatct
2401 ctgaaaacct tacgctatgg aaagattatg attatgaccg atcaggatca agatggttct
2461 cacataaaag gcctgcttat taatttcatc catcacaatt ggccatcact tttgaagcat
2521 ggttttcttg aagagttcat tactcctatt gtaaaggcaa gcaaaaataa gcaggaactt
2581 tccttctaca gtattcctga atttgacgaa tggaaaaaac atatagaaaa ccagaaagcc
2641 tggaaaataa agtactataa aggattgggt actagtacag ctaaagaagc aaaggaatat
2701 tttgctgata tggaaaggca tcgcatcttg tttagatatg ctggtcctga agatgatgct
2761 gccattacct tggcatttag taagaagaag attgatgaca gaaaagaatg gttaacaaat
2821 tttatggaag accggagaca gcgtaggcta catggcttac cagagcaatt tttatatggt
2881 actgcaacaa agcatttgac ttataatgat ttcatcaaca aggaattgat tctcttctca
2941 aactcagaca atgaaagatc tataccatct cttgttgatg gctttaaacc tggccagcgg
3001 aaagttttat ttacctgttt caagaggaat gataaacgtg aagtaaaagt tgcccagttg
3061 gctggctctg ttgctgagat gtcggcttat catcatggag aacaagcatt gatgatgact
3121 attgtgaatt tggctcagaa ctttgtggga agtaacaaca ttaacttgct tcagcctatt
3181 ggtcagtttg gaactcggct tcatggtggc aaagatgctg caagccctcg ttatattttc
3241 acaatgttaa gcactttagc aaggctactt tttcctgctg tggatgataa cctccttaag
3301 ttcctttatg atgataatca acgtgtagag cctgagtggt atattcctat aattcccatg
3361 gttttaataa atggtgctga gggcattggt actggatggg cttgtaaact acccaactat
3421 gatgctaggg aaattgtgaa caatgtcaga cgaatgctag atggcctgga tcctcatccc
3481 atgcttccaa actacaaaaa ctttaaaggc acgattcaag aacttggtca aaaccagtat
3541 gcagtcagtg gtgaaatatt tgtagtggac agaaacacag tagaaattac agagcttcca
3601 gttagaactt ggacacaggt atataaagaa caggtaagta tcaaggttac aagacaggtt
3661 taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta ttaagttatg
3721 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
3781 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
3841 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
3901 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
3961 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4021 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
4081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
4201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4261 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
4321 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
4381 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
4441 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
4501 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
4561 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
4621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
4681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
5041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
5341 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
5521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
5581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
5641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
5821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
5881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
5941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
6001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
6061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
6121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
6181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
6241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
6301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
6361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
6421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
6481 tttgctcaca tgt
//
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