| 质粒名称: | pAAVLINK-EF1a-HA-5'-SYNGAP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001380 |
| 产品用途: | 5' AAVLINK plasmid for SYNGAP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SYNGAP1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-SYNGAP1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SYNGAP1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SYNGAP1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SYNGAP1质粒载体简介
pAAVLINK-EF1a-HA-5'-SYNGAP1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SYNGAP1 5267 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5267)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5267
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2407
/codon_start=1
/label=SYNGAP1
/translation="MSRSRASIHRGSIPAMSYAPFRDVRGPSMHRTQYVHSPYDRPGWN
PRFCIISGNQLLMLDEDEIHPLLIRDRRSESSRNKLLRRTVSVPVEGRPHGEHEYHLGR
SRRKSVPGGKQYSMEGAPAAPFRPSQGFLSRRLKSSIKRTKSQPKLDRTSSFRQILPRF
RSADHDRARLMQSFKESHSHESLLSPSSAAEALELNLDEDSIIKPVHSSILGQEFCFEV
TTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRYY
CELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKKRKKDKA
GYVGLVTVPVATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGKGKGGCPA
VRLKARYQTMSILPMELYKEFAEYVTNHYRMLCAVLEPALNVKGKEEVASALVHILQST
GKAKDFLSDMAMSEVDRFMEREHLIFRENTLATKAIEEYMRLIGQKYLKDAIGEFIRAL
YESEENCEVDPIKCTASSLAEHQANLRMCCELALCK"
misc_feature 1064..1069
/label=SDS
misc_feature 1930..1935
/label=SDS
misc_feature 2079..2084
/label=SDS
misc_feature 2082..2087
/label=SDS
intron 2408..2448
/label=Splicing Donor
misc_feature 2452..2485
/label=lox2272-JT1571
repeat_region 2530..2670
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2530..2659
/label=AAV2 ITR
rep_origin 2745..3200
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3482..3586
/gene="bla"
/label=AmpR promoter
CDS 3587..4447
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4618..5206
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gagcaggtct cgagcctcca tccatcgggg
781 gagcatcccc gcgatgtcct atgccccctt cagagatgta cggggaccct ctatgcaccg
841 aacccaatac gttcattccc cgtatgatcg tcctggttgg aaccctcggt tctgcatcat
901 ctcggggaac cagctgctca tgctggatga ggatgagata caccccctac tgatccggga
961 ccggaggagc gagtccagtc gcaacaaact gctgagacgc acagtctccg tgccggtgga
1021 ggggcggccc cacggcgagc atgaatacca cttgggtcgc tcgaggagga agagtgtccc
1081 aggggggaag cagtacagca tggagggtgc ccctgctgcg cccttccggc cctcgcaagg
1141 cttcctgagc cgacggctaa aaagctccat caaacgaacg aagtcacaac ccaaacttga
1201 ccggaccagc agctttcgcc agatcctgcc tcgcttccga agtgctgacc atgaccgggc
1261 ccggctgatg caaagcttta aggagtcaca ctctcatgag tccttgctga gtcctagcag
1321 tgcagctgag gcattggagc tcaacttgga tgaagattcc attatcaagc cagtgcacag
1381 ctccatcctg ggccaggagt tctgttttga ggtaacaact tcatcaggaa caaaatgctt
1441 tgcctgtcgg tctgcggccg aaagagacaa atggattgag aatctgcagc gggcagtaaa
1501 gcccaacaag gacaacagcc gccgggtaga caatgtgcta aagctgtgga tcatagaggc
1561 ccgggagctg ccccccaaga agcggtacta ctgtgagctc tgcctggatg acatgctgta
1621 tgcacgcacc acctccaagc cccgctctgc ctctggggac accgtcttct ggggcgagca
1681 cttcgagttt aacaacctgc cggctgtccg tgccctgcgg ctgcatctgt accgtgactc
1741 agacaaaaag cgcaagaagg acaaggcagg ctatgtcggc ctggtgactg tgccagtggc
1801 caccctggct gggcgccact tcacagagca gtggtaccct gtaaccctgc caacaggcag
1861 tgggggatct gggggcatgg gttcgggagg gggagggggc tcggggggtg gctcaggggg
1921 caagggcaaa ggaggttgcc cggctgtgcg gctgaaagca cgttaccaga caatgagcat
1981 cttgcccatg gagctatata aagagtttgc agagtatgtc accaaccatt atcggatgct
2041 gtgtgcagtc ttggagcccg ccctgaatgt caaaggcaag gaggaggttg ccagtgcact
2101 agttcacatc ctgcagagta caggcaaggc caaggacttc ctttcagaca tggccatgtc
2161 tgaggtagac cggttcatgg aacgggagca cctcatattc cgcgagaaca cgcttgccac
2221 taaagccata gaagagtata tgagactgat tggtcagaaa tacctcaagg atgccattgg
2281 agaattcatc cgtgctctgt atgaatctga ggaaaactgc gaggtagacc ctatcaagtg
2341 cacagcatcc agtttggcag agcaccaggc caacctgcga atgtgctgtg agttggccct
2401 gtgcaaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
2461 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
2521 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
2581 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
2641 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
2701 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
2761 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
2821 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
2881 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
2941 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
3001 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
3061 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
3121 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
3181 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
3241 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
3301 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
3361 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
3421 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
3481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
3541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
3601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
3661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
3721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
3781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
3841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
3901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
3961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
4021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
4081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
4141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
4201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
4261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
4321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
4381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
4441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
4501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
4561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
4621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
4681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
4741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
4801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
4861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
4921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
4981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
5041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
5101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
5161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
5221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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