| 质粒名称: | pAAVLINK-EF1a-HA-5'-SETBP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001383 |
| 产品用途: | 5' AAVLINK plasmid for SETBP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETBP1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-SETBP1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SETBP1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SETBP1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SETBP1质粒载体简介
pAAVLINK-EF1a-HA-5'-SETBP1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SETBP1 7013 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7013)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7013
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4153
/codon_start=1
/label=SETBP1
/translation="ESRETLSSSRQRGGESDFLPVSSAKPPAAPGCAGEPLLSTPGPGK
GIPVGGERMEPEDEDELGSGRDVDSNSNADSEKWVAGDGLEEQEFSIKEANFTEGSLKL
KIQTTKRAKKPPKNLENYICPPEIKITIKQSGDQKVSRAGKNSKATKEEERSHSKKKLL
TASDLAASDLKGFQPQAYERPQKHSTLHYDTGLPQDFTGDTLKPKHQQKSSSQNHMDWS
TNSDSGPVTQNCFISPESGRETASTSKIPALEPVASFAKAQGKKGSAGNTWSQLSNNNK
DLLLGGVAPSPSSHSSPAPPSSSAECNGLQPLVDQDGGGTKEPPEPPTVGSKKKSSKKD
VISQTIPNPDLDWVKNAQKAFDNTEGKREGYSADSAQEASPARQNVSSASNPENDSSHV
RITIPIKAPSLDPTNHKRKKRQSIKAVVEKIMPEKALASGITMSSEVVNRILSNSEGNK
KDPRVPKLSKMIENESPSVGLETGGNAEKVIPGGVSKPRKPPMVMTPPTCTDHSPSRKL
PEIQHPKFAAKRRWTCSKPKPSTMLREAVMATSDKLMLEPPSAYPITTSSPLYTNTDSL
TVITPVKKKRGRPKKQPLLTVETIHEGTSTSPVSPISREFPGTKKRKRRRNLAKLAQLV
PGEDKPMSEMKFHKKVGKLGVLDKKTIKTINKMKTLKRKNILNQILSCSSSVALKAKAP
PETSPGAAAIESKLGKQINVSKRGTIYIGKKRGRKPRAELPPPSEEPKTAIKHPRPVSS
QPDVPAVPSNFQSLVASSPAAMHPLSTQLGGSNGNLSPASTETNFSELKTMPNLQPISA
LPTKTQKGIHSGTWKLSPPRLMANSPSHLCEIGSLKEITLSPVSESHSEETIPSDSGIG
TDNNSTSDQAEKSSESRRRYSFDFCSLDNPEAIPSDTSTKNRHGHRQKHLIVDNFLAHE
SLKKPKHKRKRKSLQNRDDLQFLADLEELITKFQVFRISHRSYTFYHENPYPSIFRINF
DHYYPVPYIQYDPLLYLRRTSDLKSKKKRGRPAKTNDTMTKVPFLQGFSYPIPSGSYYA
PYGMPYTSMPMMNLGYYGQYPAPLYLSHTLGAASPFMRPTVPPPQFHTNSHVKMSGAAK
HKAKHGVHLQGPVSMGLGDMQPSLNPPK"
misc_feature 918..923
/label=SDS
misc_feature 921..926
/label=SDS
misc_feature 1026..1031
/label=SDS
misc_feature 1200..1205
/label=SDS
misc_feature 1702..1707
/label=SDS
misc_feature 2218..2223
/label=SDS
misc_feature 3413..3418
/label=SDS
intron 4154..4194
/label=Splicing Donor
misc_feature 4198..4231
/label=lox2272-JT1571
repeat_region 4276..4416
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4276..4405
/label=AAV2 ITR
rep_origin 4491..4946
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5228..5332
/gene="bla"
/label=AmpR promoter
CDS 5333..6193
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6364..6952
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctga gtccagggaa accttaagca gctcccggca
781 aagagggggc gagtcagact tcctgccggt ctcctcagcc aagcccccag ctgctcctgg
841 ctgtgcagga gaacctttgc tctccactcc aggacctggg aaggggatcc cggtgggcgg
901 agagcgcatg gagccagagg acgaggatga actaggctca gggcgggatg tggattccaa
961 ctccaacgcg gacagtgaga aatgggtggc aggagatggt ttggaagagc aggaattttc
1021 tatcaaggag gcaaacttca cagagggaag tctgaagcta aagattcaga ccacaaagcg
1081 ggctaagaaa cccccaaaga atttggagaa ctatatatgt ccacctgaga tcaagatcac
1141 catcaagcag tctggggacc agaaggtgtc ccgtgctgga aaaaatagca aagccacgaa
1201 ggaggaagaa agaagccact ccaaaaagaa gctcctcaca gccagtgacc ttgcagccag
1261 tgacctcaaa ggatttcagc cacaggctta cgagaggccc cagaaacatt caactctcca
1321 ttatgacacg ggcctcccac aggacttcac cggtgacacc ttaaaaccaa agcaccagca
1381 aaaaagcagc agccagaacc acatggactg gtccaccaac tctgacagcg gacccgtcac
1441 tcagaattgc ttcatcagtc cagagtctgg cagagaaact gcaagcacca gcaagatccc
1501 cgctcttgag cccgtggctt cctttgcaaa ggcccagggt aagaaaggca gtgcagggaa
1561 cacgtggagt cagttgtcta acaataacaa agatctgctc ttgggaggtg tggctccatc
1621 cccaagcagc cacagctcac cagccccacc cagcagctct gctgagtgca acgggcttca
1681 gcccttggtg gatcaagatg gaggaggtac aaaggagccc ccagaaccac ctacggtggg
1741 cagcaagaaa aagtccagta aaaaagatgt gataagtcag accataccaa acccagacct
1801 ggattgggtc aagaatgccc agaaagcatt tgacaataca gaagggaaaa gggaaggtta
1861 ttccgcagat agtgcccaag aggcatcacc agccaggcag aacgtgagtt ctgccagtaa
1921 tcctgaaaat gactcaagtc atgtccggat tactatcccc atcaaggcac cctctctgga
1981 tccaaccaac cataagagga aaaaaagaca gtccattaaa gcggtggtgg aaaagatcat
2041 gccagagaaa gccttggctt ctggaatcac catgagcagt gaagtagtta acaggatact
2101 ttccaactct gaggggaata agaaggatcc ccgtgtccct aagttgagta aaatgataga
2161 gaatgagtcc ccctcagttg gccttgaaac tggtggaaat gctgagaaag ttatcccagg
2221 aggtgtgtct aagccgcgga agccacccat ggtcatgaca cctccaacgt gcacagatca
2281 ctctccatcc agaaagctgc cagaaatcca gcatccaaaa tttgctgcaa aacgaaggtg
2341 gacttgcagc aaaccaaaac ctagcaccat gcttcgagag gcagttatgg ccacctctga
2401 taaactgatg ctggagcccc cgtctgcata tcccatcacc acatccagcc ctctctacac
2461 caacacagac agtcttactg tgatcactcc agtcaaaaag aagcggggac gaccaaagaa
2521 gcagcctttg cttacagtcg agacgattca tgagggaact tccaccagcc ccgtcagtcc
2581 catcagccga gagtttcctg gcactaagaa aagaaagcga cgacgcaatt tagcgaagtt
2641 ggcccagcta gtgccgggag aggacaaacc catgagcgag atgaaatttc acaagaaagt
2701 tggaaagctc ggcgtgttgg ataagaagac catcaaaact atcaataaga tgaagacact
2761 caagaggaaa aacatcttga atcagatctt gtcctgttcc agcagcgttg ctctgaaggc
2821 aaaagctccc ccagagacca gccctggggc agcagccatt gaaagcaaac tgggcaagca
2881 gattaatgtc agcaagaggg gaaccatcta cattggcaag aagcggggca ggaagccaag
2941 agcagagctg ccacccccat ccgaagaacc caaaacagcc atcaagcacc ccaggcctgt
3001 ttctagccag ccggatgttc cagccgtgcc ttccaacttt cagtcacttg tggcgtcttc
3061 accagcagct atgcacccac tttcaacaca gttaggtggg tccaatggca acctgagccc
3121 tgccagcact gaaaccaatt tttcagagtt gaaaactatg ccaaatctcc agcccatcag
3181 tgctcttcca accaaaaccc aaaagggaat acacagtgga acctggaagc tgtctccacc
3241 cagactgatg gccaactccc cttcacacct gtgcgagatt ggctccctaa aggaaatcac
3301 gctgtcccct gtgagcgagt cccacagtga ggagacgatc cccagcgaca gcggcattgg
3361 gacagacaac aacagcactt ctgaccaagc ggagaagagc tcagaatccc gaaggaggta
3421 ctcttttgat ttctgctccc tggacaaccc ggaggccatt ccgtccgaca ccagcacaaa
3481 gaaccggcat ggccaccggc aaaagcatct cattgtggac aactttctgg cccacgaaag
3541 cctcaagaag ccaaagcaca agaggaaacg gaaaagcctg caaaaccgcg atgacctcca
3601 gtttctggca gacctggagg agctaatcac caagttccaa gtgttcagaa tctcccaccg
3661 gagttacacc ttctaccacg agaatccata tcccagcatt tttcggatta attttgatca
3721 ctattacccg gtgccatata tccagtatga cccgttgctc tatcttcgta ggacttcaga
3781 cttgaagtca aagaagaagc gtggtaggcc tgcaaaaacc aatgacacca tgacaaaggt
3841 gcctttttta caagggttca gctaccctat tcccagtgga agttactatg caccctatgg
3901 aatgccttac acatcaatgc ctatgatgaa ccttggttat tacggtcagt acccagctcc
3961 tttgtaccta tcgcacacgc ttggagcagc ttccccattc atgaggccaa cagtgccacc
4021 acctcagttc cacacaaact cccacgtaaa gatgtccggt gcagctaagc ataaagccaa
4081 gcatggagta cacctgcagg gacctgttag catgggcctt ggtgacatgc agccttctct
4141 gaaccctccc aaggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
4201 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
4261 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4321 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4381 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
4441 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4561 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4681 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4801 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4861 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4921 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4981 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
5041 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
5101 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
5161 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
5221 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
5281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
5341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
5401 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
5461 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
5521 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
5581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5641 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
5701 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
5761 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
5821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5881 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
6001 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
6061 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
6121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
6181 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
6241 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
6301 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
6361 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
6421 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
6481 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
6541 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6601 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6661 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
6721 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6781 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6841 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6901 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6961 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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