| 质粒名称: | pAAVLINK-EF1a-HA-5'-PREX1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001521 |
| 产品用途: | 5' AAVLINK plasmid for PREX1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PREX1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-PREX1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PREX1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PREX1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PREX1质粒载体简介
pAAVLINK-EF1a-HA-5'-PREX1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PREX1 7043 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7043)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7043
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4183
/codon_start=1
/label=PREX1
/translation="EAPSGSEPGGDGAGDCAHPDPRAPGAAAPSSGPGPCAAARESERQ
LRLRLCVLNEILGTERDYVGTLRFLQSAFLHRIRQNVADSVEKGLTEENVKVLFSNIED
ILEVHKDFLAALEYCLHPEPQSQHELGNVFLKFKDKFCVYEEYCSNHEKALRLLVELNK
IPTVRAFLLSCMLLGGRKTTDIPLEGYLLSPIQRICKYPLLLKELAKRTPGKHPDHPAV
QSALQAMKTVCSNINETKRQMEKLEALEQLQSHIEGWEGSNLTDICTQLLLQGTLLKIS
AGNIQERAFFLFDNLLVYCKRKSRVTGSKKSTKRTKSINGSLYIFRGRINTEVMEVENV
EDGTADYHSNGYTVTNGWKIHNTAKNKWFVCMAKTAEEKQKWLDAIIREREQRESLKLG
MERDAYVMIAEKGEKLYHMMMNKKVNLIKDRRRKLSTVPKCFLGNEFVAWLLEIGEISK
TEEGVNLGQALLENGIIHHVSDKHQFKNEQVMYRFRYDDGTYKARSELEDIMSKGVRLY
CRLHSLYTPVIKDRDYHLKTYKSVLPGSKLVDWLLAQGDCQTREEAVALGVGLCNNGFM
HHVLEKSEFRDESQYFRFHADEEMEGTSSKNKQLRNDFKLVENILAKRLLILPQEEDYG
FDIEEKNKAVVVKSVQRGSLAEVAGLQVGRKIYSINEDLVFLRPFSEVESILNQSFCSR
RPLRLLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKV
NGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQ
AQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEK
IVEPRGCFGLTAKILEAFAANDSVFVENCRRLMALSSAIVTMPHFEFRNICDTKLESIG
QRIACYQEFAAQLKSRVSPPFKQAPLEPHPLCGLDFCPTNCHINLMEVSYPKTTPSVGR
SFSIRFGRKPSLIGLDPEQGHLNPMSYTQHCITTMAAPSWKCLPAAEGDPQGQGLHDGS
FGPASGTLGQEDRGLSFLLKQEDREIQDAYLQLFTKLDVALKEMKQYVTQINRLLSTIT
EPTSGGSCDASLAEEASSLPLVSEESEMDRSDHGGIKK"
misc_feature 1357..1362
/label=SDS
misc_feature 1561..1566
/label=SDS
misc_feature 2430..2435
/label=SDS
misc_feature 2637..2642
/label=SDS
misc_feature 2640..2645
/label=SDS
misc_feature 2842..2847
/label=SDS
misc_feature 3150..3155
/label=SDS
misc_feature 3189..3194
/label=SDS
misc_feature 3828..3833
/label=SDS
misc_feature 3954..3959
/label=SDS
misc_feature 4110..4115
/label=SDS
misc_feature 4116..4121
/label=SDS
intron 4184..4224
/label=Splicing Donor
misc_feature 4228..4261
/label=lox2272-JT1571
repeat_region 4306..4446
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4306..4435
/label=AAV2 ITR
rep_origin 4521..4976
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5258..5362
/gene="bla"
/label=AmpR promoter
CDS 5363..6223
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6394..6982
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctga ggcgcccagc ggcagcgagc ccggcggcga
781 cggggccggg gactgcgccc acccggaccc ccgggcccct ggcgccgcgg cgcccagctc
841 cggccccggc ccgtgcgcgg ccgcccggga gtccgagcgc cagctgcgcc tccgcctctg
901 cgtcctcaac gagatcttgg gcaccgagag ggactacgtg ggcaccttgc gcttcttgca
961 gtcggcattc ctgcatcgca tccggcagaa cgtggccgac tcagtggaga agggcctcac
1021 ggaggagaat gtcaaggtcc tgttctcgaa catcgaagac atcctggaag ttcataagga
1081 tttcttggcc gccttggagt attgtttaca cccggagccg cagtctcagc atgaacttgg
1141 gaatgttttc ttaaaattca aggacaagtt ctgcgtgtac gaggagtatt gcagcaacca
1201 tgagaaagcc ctgaggctgc tggtggagct gaacaagatc cctaccgtgc gcgccttcct
1261 tttgagctgc atgcttctgg gaggccggaa gaccacggac atccctttgg aaggctacct
1321 gttgtctccg atccagagga tctgcaagta cccgctcctc cttaaggagc tggccaagag
1381 gactcccggc aagcacccag accaccccgc ggtccagagt gccctgcagg ccatgaagac
1441 cgtttgctcc aacatcaatg agaccaagcg gcagatggag aagctggaag ccctggagca
1501 gctgcagtcc cacatcgaag gctgggaggg ttccaacctc acagacatct gcactcagct
1561 cctcctgcaa gggactttgt taaagatctc tgcgggcaac atccaggaaa gggccttctt
1621 cctcttcgac aaccttctcg tctactgcaa gcggaaatcc agggtcaccg ggagcaagaa
1681 gtccaccaag aggaccaaat ccatcaacgg ctccctctac atcttcaggg gtcgaatcaa
1741 cactgaagtc atggaggtgg agaatgtgga agatgggaca gcggattacc atagcaacgg
1801 ctataccgtc accaacggct ggaagatcca caacacggcc aagaataagt ggtttgtctg
1861 catggccaag acggcagagg agaagcagaa gtggctggat gccatcatcc gcgagcggga
1921 gcagcgcgag agcctgaagc tgggcatgga gcgtgatgcc tacgtcatga ttgcggagaa
1981 gggggagaag ctgtaccaca tgatgatgaa caagaaggtg aacctcatca aggaccgccg
2041 gagaaagctg agcactgtcc ccaagtgctt tcttggcaat gagttcgttg cctggctcct
2101 agaaattggt gaaatcagca agacggaaga aggagtcaac ttgggccaag ccctgttgga
2161 gaatggcatc atccaccatg tttccgacaa gcaccagttc aagaatgagc aggtgatgta
2221 tcgcttccgc tacgacgatg gcacctacaa ggcccgaagt gagctggagg acatcatgtc
2281 caagggtgtg aggctttact gccgtcttca cagcctctac accccggtga tcaaagaccg
2341 tgattaccac ctgaagacct acaagtcagt gcttcccggg agcaagctgg tggactggct
2401 gctggctcag ggagactgcc agactcggga ggaggcagtg gcgctcggcg tgggtctgtg
2461 caacaatggc ttcatgcacc acgtgctgga gaagagcgag ttcagggatg agtcccagta
2521 cttccgcttt catgctgacg aggagatgga ggggaccagc agcaagaaca aacagcttcg
2581 caacgacttc aagctggtgg agaacattct ggccaagcgc ctgctgatcc tgccccagga
2641 ggaggactat ggctttgaca tcgaggagaa gaacaaggct gtggtggtga agtccgtcca
2701 gaggggctcg ctggctgagg tggctggcct gcaggtgggg aggaagatct actccatcaa
2761 tgaggacctg gtgttcctgc ggccgttttc agaggtggag tccatcctca accagtcctt
2821 ctgctcccgc cgccctctgc gcctcctggt ggccacgaag gccaaagaga tcatcaaaat
2881 ccccgaccag ccggacacac tgtgcttcca gattcgtgga gctgccccac cgtacgtcta
2941 tgctgtgggg agaggctctg aggccatggc tgcagggctc tgtgctggtc agtgcattct
3001 gaaggtcaat ggcagcaacg tgatgaacga tggtgcccct gaggtcctgg agcacttcca
3061 ggcattccgg agtcggcgcg aagaggccct gggcctgtac cagtggatct accacaccca
3121 tgaggatgcc caggaagcac gagccagtca ggaggcctcc actgaggacc ccagtggcga
3181 gcaggcccag gaggaagacc aggctgattc agccttccca ctgctgtccc tgggtccccg
3241 gctgagcctg tgtgaggaca gccccatggt caccctgact gtggacaacg tgcacctgga
3301 acacggcgtg gtgtatgagt atgtgagcac ggcaggcgtc aggtgccatg tgctggagaa
3361 gatcgtggag ccccgcggct gcttcggcct caccgccaag atcctcgagg cctttgctgc
3421 caatgacagc gtcttcgtgg agaactgcag gcggctcatg gccctgagca gcgccatcgt
3481 gaccatgccc cactttgagt tccgcaacat ctgtgacacc aagctggaga gcattggcca
3541 gaggattgcc tgctaccagg agtttgcagc ccaactgaag agcagggtca gcccaccctt
3601 caaacaagcc cccctggagc cccacccgct gtgtggcctg gacttctgcc ccaccaattg
3661 ccacatcaac ctcatggaag tgtcctaccc caagaccacc ccctcagtgg gcaggtcctt
3721 cagcatccgc tttggacgca aaccctccct catcggcctt gacccggagc aaggccacct
3781 gaaccccatg tcgtacaccc agcactgcat caccaccatg gctgctccct cctggaagtg
3841 cttgcctgct gcagagggtg atccccaagg ccagggtctc catgatggca gcttcgggcc
3901 agccagtggg acccttggtc aggaagaccg gggcctcagc ttcctactca agcaggagga
3961 ccgtgagatc caggatgcct acctgcagct cttcaccaag ctggatgtgg ccctgaagga
4021 gatgaagcaa tatgtcaccc agatcaacag gctgctgtcc accatcacag agcccacctc
4081 gggtgggtcc tgcgacgcat ccttggctga ggaggcctcc tccctgcccc tggtcagtga
4141 agagagcgag atggacagga gtgaccatgg gggcatcaag aaggtaagta tcaaggttac
4201 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
4261 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
4321 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
4381 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
4441 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
4501 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
4561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
4621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
4741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
4801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
4861 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
4921 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
4981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
5041 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
5101 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
5161 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
5221 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
5281 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
5341 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
5401 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
5461 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
5521 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
5581 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
5641 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
5701 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
5761 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
5821 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
5881 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
5941 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
6001 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
6061 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
6121 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
6181 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
6241 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
6301 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
6361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
6421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
6481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
6541 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
6601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
6661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
6721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
6781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
6841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
6901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
6961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
7021 ttgctggcct tttgctcaca tgt
//
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pAAVLINK-EF1a-HA-5'-PREX1质粒载体应用举例
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