| 质粒名称: | pAAVLINK-EF1a-HA-5'-NUP155 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001401 |
| 产品用途: | 5' AAVLINK plasmid for NUP155 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NUP155 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-NUP155载体质粒基本信息
pAAVLINK-EF1a-HA-5'-NUP155质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-NUP155载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-NUP155质粒载体简介
pAAVLINK-EF1a-HA-5'-NUP155质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-NUP155 5716 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5716)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5716
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..2878
/codon_start=1
/label=NUP155
/translation="PSSLLGAAMPASTSAAALQEALENAGRLIDRQLQEDRMYPDLSEL
LMVSAPNNPTVSGMSDMDYPLQGPGLLSVPNLPEISSIRRVPLPPELVEQFGHMQCNCM
MGVFPPISRAWLTIDSDIFMWNYEDGGDLAYFDGLSETILAVGLVKPKAGIFQPHVRHL
LVLATPVDIVILGLSYANLQTGSGVLNDSLSGGMQLLPDPLYSLPTDNTYLLTITSTDN
GRIFLAGKDGCLYEVAYQAEAGWFSQRCRKINHSKSSLSFLVPSLLQFTFSEDDPILQI
AIDNSRNILYTRSEKGVIQVYDLGQDGQGMSRVASVSQNAIVSAAGNIARTIDRSVFKP
IVQIAVIENSESLDCQLLAVTHAGVRLYFSTCPFRQPLARPNTLTLVHVRLPPGFSASS
TVEKPSKVHRALYSKGILLMAASENEDNDILWCVNHDTFPFQKPMMETQMTAGVDGHSW
ALSAIDELKVDKIITPLNKDHIPITDSPVVVQQHMLPPKKFVLLSAQGSLMFHKLRPVD
QLRHLLVSNVGGDGEEIERFFKLHQEDQACATCLILACSTAACDREVSAWATRAFFRYG
GEAQMRFPTTLPPPSNVGPILGSPVYSSSPVPSGSPYPNPSFLGTPSHGIQPPAMSTPV
CALGNPATQATNMSCVTGPEIVYSGKHNGICIYFSRIMGNIWDASLVVERIFKSGNREI
TAIESSVPCQLLESVLQELK"
misc_feature 1061..1066
/label=SDS
misc_feature 1222..1227
/label=SDS
misc_feature 1910..1915
/label=SDS
misc_feature 2618..2623
/label=SDS
intron 2879..2919
/label=Splicing Donor
misc_feature 2923..2956
/label=lox2272-JT1571
repeat_region 3001..3119
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3001..3108
/label=AAV2 ITR
rep_origin 3194..3649
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3931..4035
/gene="bla"
/label=AmpR promoter
CDS 4036..4896
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5067..5655
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctccgt cttctttgtt gggtgcggcg atgccggcct ctacatctgc
781 cgcagccctg caggaagctc tggaaaatgc tggacggctc atcgaccgtc agttgcaaga
841 ggaccgcatg tacccggacc tttccgagct gcttatggtg tctgccccaa ataatcccac
901 cgtttctggc atgtcagata tggattatcc tttacaagga cctggtttgc tgtccgtacc
961 caaccttcca gagatcagtt ccatccgaag agttcctctc ccacctgaac ttgttgagca
1021 gtttggacat atgcagtgta attgcatgat gggtgtgttc cctcctatca gcagagcttg
1081 gctcacaatt gacagtgata tattcatgtg gaactatgag gatggaggag accttgccta
1141 ttttgatgga cttagtgaga ctattcttgc tgtggggctt gtgaagccaa aagcaggcat
1201 ctttcaacct catgtgcgac acctcctggt tttggcgacc cctgtagaca tagtaattct
1261 tggactcagc tatgctaatt tgcaaacagg ttctggagtt cttaatgata gtttgtctgg
1321 tggaatgcag ttgcttccag atcctttata ttctcttcct actgataata cttacctttt
1381 aacaataact tccactgata atggcagaat tttcttggct ggaaaggatg gctgtttata
1441 tgaagtagcc taccaggctg aagcagggtg gtttagccaa agatgtagga aaataaacca
1501 ctcaaagagc tcactttctt tccttgttcc ttccttgcta caattcacgt tctcagaaga
1561 tgatcctatt cttcaaattg caattgataa ttctagaaat attttatata cacgatctga
1621 gaaaggagta atacaggtgt atgatttggg acaagatgga caaggaatga gcagagttgc
1681 ctctgtgtca cagaatgcca ttgtctctgc tgctggtaac attgctagga ccatcgatcg
1741 ttctgttttt aaaccaattg tccaaatagc agtgattgaa aattctgaat cactggactg
1801 tcagttattg gctgtcacac atgcaggtgt taggttatat tttagcactt gtccattcag
1861 acagccatta gcacggccta atacactgac gctggttcat gtccgcttac ctcctggatt
1921 ctcagcatct tcaaccgtgg aaaagccttc aaaagtacat agagctcttt atagtaaagg
1981 tattctattg atggcagcct cagaaaatga ggataatgat attttatggt gtgtcaacca
2041 tgatactttt cctttccaaa agccaatgat ggaaacccag atgacagctg gtgttgatgg
2101 tcattcctgg gctctttctg cgatagatga attgaaagta gataaaataa ttacaccttt
2161 aaacaaggat catattccaa taactgattc accagttgtt gtacagcagc acatgttacc
2221 tccgaagaaa tttgttctcc tctcagcaca ggggagcctt atgtttcata aacttagacc
2281 tgtagatcaa ctgaggcatc tacttgtgag taatgtggga ggagatggag aagagattga
2341 aagattcttt aaattacatc aggaagacca ggcttgtgca acttgcctta ttcttgcttg
2401 ctccactgct gcctgtgata gagaagtatc tgcctgggct actcgggctt tctttaggta
2461 tggtggtgaa gcacagatga gatttccaac cactcttccg cctccaagta atgttggtcc
2521 catcttgggg tctcctgtct attctagttc tcctgttcct agtggtagtc cctatccaaa
2581 tccatccttt ttgggaacac cgtctcatgg tatacagcct cctgccatgt caactccagt
2641 gtgtgctctg ggaaacccag caactcaggc cacaaatatg agttgtgtga ctggaccaga
2701 gattgtgtac tctggaaaac acaatggtat ttgcatttac ttttctcgga tcatgggaaa
2761 catttgggat gcaagcttag ttgtggagag aatattcaag agtggcaaca gagagatcac
2821 tgcaattgaa agtagtgttc cctgccaact gctagagtca gtgctacaag aactaaaggt
2881 aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc gtataggata
2941 ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc
3001 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
3061 ccgggcgacc aaaggtcgcc cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
3121 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
3181 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
3241 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
3301 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
3361 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
3421 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
3481 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
3541 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
3601 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
3661 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
3721 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
3781 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
3841 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
3901 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
3961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
4081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
4141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
4201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
4261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
4321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
4381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
4441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
4501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
4561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
4621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
4681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
4741 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
4801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
4861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
4921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
4981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
5041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
5101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
5161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
5221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
5281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
5341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
5401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
5461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
5521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
5581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
5641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
5701 ccttttgctc acatgt
//
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