| 质粒名称: | pAAVLINK-EF1a-HA-5'-MBD5 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001362 |
| 产品用途: | 5' AAVLINK plasmid for MBD5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MBD5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-MBD5载体质粒基本信息
pAAVLINK-EF1a-HA-5'-MBD5质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-MBD5载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-MBD5质粒载体简介
pAAVLINK-EF1a-HA-5'-MBD5质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-MBD5 7231 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7231)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7231
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4393
/codon_start=1
/label=MBD5
/translation="NGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSC
LEQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRK
IIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSVMPEC
KNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSHGGLPS
PASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVMLHG
SPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPP
PSCALQKKPLTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAV
VPLPSNLPLPTVKPGHMNHGSHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQR
SRSSSTSSDHGNFMMPPVGPQATSSGIKVPPRSPRSTIGSPRPSMPSSPSTKSDGHHQY
KDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGMPLNQILNQHNAASFPAS
SLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANMLLPTGE
GQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGS
FPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPL
RGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQ
SRISTSSTPVIPNSIVSSYNQTSSEAGGSGPSSSIAIAGTNHPAITKTTSVLQDGVIVT
TAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHLPHPLNPSLLSSLPISLPVNQQ
HLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNAALAFLSSDMDGQVLQPVHFQLLA
ALLQNQAQAAAMLPLPSFNLTISDLLQQQNTPLPSLTQMTAPPDHLPSNQSDNSRAETL
LTSPLGNPLPSFAGSDTTFNPLFLPAVNGASGLMTLNPQLLGGVLNSASANTANHPEVS
IATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAETLLNISNNAGNTPGPAKLNSNSV
VPQLLNPLLGTGLLGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQQQQQLLQ"
misc_feature 772..777
/label=SDS
misc_feature 1120..1125
/label=SDS
misc_feature 1748..1753
/label=SDS
misc_feature 1922..1927
/label=SDS
misc_feature 2448..2453
/label=SDS
misc_feature 2606..2611
/label=SDS
misc_feature 3484..3489
/label=SDS
misc_feature 4065..4070
/label=SDS
intron 4394..4434
/label=Splicing Donor
misc_feature 4438..4471
/label=lox2272-JT1571
repeat_region 4516..4634
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4516..4623
/label=AAV2 ITR
rep_origin 4709..5164
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5446..5550
/gene="bla"
/label=AmpR promoter
CDS 5551..6411
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6582..7170
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctaatg gaggcaaaga gtgtgacgga ggggacaagg aaggaggtct
781 tccagctata caagttcctg tgggttggca gcgtcgtgtg gatcaaaatg gagtgcttta
841 tgtcagtccc agtgggtctt tgttatcttg cttggagcag gttaaaacat acctgcttac
901 tgatggaaca tgcaagtgtg gcttggaatg tcctcttatt cttcccaagg tatttaattt
961 tgatcctgga gctgctgtga aacagagaac cgcagaagat gttaaggcag atgaagatgt
1021 cacaaagcta tgcatacata aaagaaaaat tattgcagtg gccacacttc ataaaagcat
1081 ggaagcccca catccttctc tggtgctcac cagtcccgga ggaggaacaa atgcaactcc
1141 agtagtacct tctcgggcag caactccaag atcagtaaga aataagtctc atgaaggaat
1201 tacaaattct gtaatgcctg aatgtaagaa tcctttcaag ttaatgattg gatcatcaaa
1261 tgccatggga aggctatatg tacaagaact gcctggaagc caacaacaag aactccaccc
1321 tgtctacccc cgacagagat tgggcagcag tgaacatgga cagaaatctc cattccgtgg
1381 cagccatgga ggcctgccca gcccagcgtc atcaggttcc cagatatatg gagatggttc
1441 aatctctcca aggactgacc cacttggaag tcctgatgtt ttcacaagaa gtaatcctgg
1501 ttttcatgga gctcccaatt ctagtcctat tcacctgaat aggactcctc tttctccacc
1561 ttcagtaatg ctacatggtt ctcctgtaca gtcatcctgt gcaatggctg gaaggactaa
1621 tatacctctt tccccaacct tgactacaaa gagtccagta atgaaaaaac caatgtgtaa
1681 tttttcaact aatatggaaa taccacgagc aatgttccac cacaaaccac cccaaggccc
1741 acctccccct cctccacctt cttgtgctct tcagaaaaag ccattaacat ctgagaaaga
1801 tccacttggc attcttgacc ctattcctag taaaccagtg aatcagaacc ctgttatcat
1861 taatccaacc agtttccatt caaatgtcca ctctcaggta cctatgatga atgtaagcat
1921 gcctcctgct gttgttcctt tgccaagtaa tctcccattg ccaactgtaa aacctggtca
1981 catgaatcat gggagtcatg tacaaagagt tcagcattca gcttcaacct ccctgtcccc
2041 ttctccagtg acatcccccg tgcacatgat ggggactgga attggaagga ttgaggcatc
2101 gccccaaaga tcacgctcat cttccacatc atcagatcat ggaaatttca tgatgccacc
2161 tgtaggaccc caggccactt ctagtggtat taaggttcca cccaggtcac caaggtcaac
2221 aatagggtcc ccaaggccat caatgccatc aagcccttct accaagtccg atggacatca
2281 tcagtacaag gatatcccta acccattaat tgctggaata agtaatgtac taaatacccc
2341 aagcagtgca gcttttccta ctgcatctgc cggaagtagt tctgtaaaga gtcagcctgg
2401 tttgctggga atgcctttaa atcagatctt gaaccagcac aatgctgcct cctttccagc
2461 aagtagttta ctctcagcag cagccaaagc acagctagca aatcaaaaca aacttgctgg
2521 taacaacagt agcagcagta gcaattctgg agctgttgcc ggcagtggca acactgaagg
2581 acatagcact ttaaacacca tgttccctcc tactgccaac atgcttctcc caacaggtga
2641 agggcaaagt ggtcgagcag cactaagaga taagctgatg tctcagcaaa aagacgcatt
2701 gcggaaaaga aaacaaccac ctacgacagt gttgagtttg ctcagacagt ctcaaatgga
2761 tagttctgca gttcctaaac ctggacctga cttgctaagg aagcagggtc agggttcatt
2821 tcccatcagt tcaatgtctc agttactaca gtctatgagt tgtcaaagct ctcacttgag
2881 tagcaatagt accccgggtt gtggggcctc aaatactgct ttgccttgct ctgctaacca
2941 gctgcatttt acagatccca gtatgaactc tagtgttctt cagaacatac ctttaagagg
3001 ggaagccgtg cactgccaca atgcaaacac taactttgtt cacagtaaca gtccagtccc
3061 caaccaccat cttgcaggtt taataaatca gattcaggct agcgggaact gtgggatgct
3121 cagtcagtcg ggcatggctt taggaaattc cttacatccc aatccacctc agtcaagaat
3181 ttcaacgtcc tccactccag tgataccaaa cagcattgtt agcagctata atcaaacaag
3241 ttctgaagca ggcggttcag gaccatcatc ctccatagcc atagcgggca ccaaccaccc
3301 tgccatcaca aagacaacat ctgttcttca agatggcgtc atagtcacca ctgcagctgg
3361 aaacccactg cagagtcagc tacccattgg gagtgatttt ccttttgttg gccaggagca
3421 cgcacttcat tttccatcca acagcacttc aaacaaccat cttccacacc ccttgaaccc
3481 cagcctcctc agttctctac ctatctcttt gccagtgaat caacagcatc tcctaaacca
3541 gaatctatta aatatcctcc agccttcagc aggagaaggc aagtctgaga tcaacctcca
3601 ccctttaggt tttctcaacc cgaatgtaaa cgctgcttta gcttttctct ccagtgacat
3661 ggatgggcag gtattgcagc ctgttcactt tcagctctta gcagccttgc ttcagaacca
3721 agcccaagca gctgccatgc ttcccctgcc atctttcaat ctgaccatct cagatctttt
3781 gcaacagcaa aatacccctt taccctcatt aacacagatg acagccccac cagaccattt
3841 gccaagcaat cagtcagaca acagccgagc tgagaccctt ttaaccagcc ccctggggaa
3901 ccctttacca agctttgcag gcagtgacac tacttttaac cccctgttcc tcccagctgt
3961 caatggggcc tcaggattaa tgaccttgaa tccccagctg ttgggaggtg tcctgaactc
4021 ggcatcggcc aacaccgcta atcatccaga ggtttccata gcaacctcct cccaggcaac
4081 cactaccaca accactacat catcagcagt ggcagcactg actgtctcaa cacttggtgg
4141 gacagcagtg gtgtcaatgg cagaaacatt gctgaatata tctaataatg ctgggaatac
4201 acctggtcca gctaaactca acagtaactc tgtggtgcca cagctactta accctctact
4261 ggggacaggt ctacttggtg atatgtcatc aataaacaat actttgagta accatcaact
4321 gactcatcta cagtcgctgt taaacaacaa tcagatgttt cctccaaatc agcaacagca
4381 gcaacttctc caggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
4441 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
4501 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4561 gctcgctcac tgaggccggg cgaccaaagg tcgcccggcc tcagtgagcg agcgagcgcg
4621 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
4681 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
4741 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
4801 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
4861 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
4921 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
4981 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
5041 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
5101 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
5161 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
5221 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
5281 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
5341 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
5401 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
5461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
5521 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
5581 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
5641 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
5701 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
5761 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
5821 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
5881 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
5941 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
6001 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
6061 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
6121 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
6181 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
6241 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
6301 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
6361 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
6421 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
6481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
6541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
6601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
6661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
6721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
6781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
6841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
6901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
6961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
7021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
7081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
7141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
7201 ctggcctttt gctggccttt tgctcacatg t
//
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