| 质粒名称: | pAAVLINK-EF1a-HA-5'-KIAA0232 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001425 |
| 产品用途: | 5' AAVLINK plasmid for KIAA0232 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KIAA0232 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-KIAA0232载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KIAA0232质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KIAA0232载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KIAA0232质粒载体简介
pAAVLINK-EF1a-HA-5'-KIAA0232质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KIAA0232 6060 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6060)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6060
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3200
/codon_start=1
/label=KIAA0232
/translation="YPICTVVVDGLPSESSSSSYPGPVSVSEMSLLHALGPVQTWLGQE
LEKCGIDAMIYTRYVLSLLLHDSYDYDLQEQENDIFLGWEKGAYKKWGKSKKKCSDLTL
EEMKKQAAVQCLRSASDESSGIETLVEELCSRLKDLQSKQEEKIHKKLEGSPSPEAELS
PPAKDQVEMYYEAFPPLSEKPVCLQEIMTVWNKSKVCSYSSSSSSSTAPPASTDTSSPK
DCNSESEVTKERSSEVPTTVHEKTQSKSKNEKENKFSNGTIEEKPALYKKQIRHKPEGK
IRPRSWSSGSSEAGSSSSGNQGELKASMKYVKVRHKAREIRNKKGRNGQSRLSLKHGEK
AERNIHTGSSSSSSSGSVKQLCKRGKRPLKEIGRKDPGSTEGKDLYMENRKDTEYKEEP
LWYTEPIAEYFVPLSRKSKLETTYRNRQDTSDLTSEAVEELSESVHGLCISNNNLHKTY
LAAGTFIDGHFVEMPAVINEDIDLTGTSLCSLPEDNKYLDDIHLSELTHFYEVDIDQSM
LDPGASETMQGESRILNMIRQKSKENTDFEAECCIVLDGMELQGERAIWTDSTSSVGAE
GLFLQDLGNLAQFWECCSSSSGDADGESFGGDSPVRLSPILDSTVLNSHLLAGNQELFS
DINEGSGINSCFSVFEVQCSNSVLPFSFETLNLGNENTDSSANMLGKTQSRLLIWTKNS
AFEENEHCSNLSTRTCSPWSHSEETRSDNETLNIQFEESTQFNAEDINYVVPRVSSNYV
DEELLDFLQDETCQQNSRTLGEIPTLVFKKTSKLESVCGIQLEQKTENKNFETTQVCNE
SPHGDGYSS"
intron 3201..3241
/label=Splicing Donor
misc_feature 3245..3278
/label=lox2272-JT1571
repeat_region 3323..3463
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3323..3452
/label=AAV2 ITR
rep_origin 3538..3993
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4275..4379
/gene="bla"
/label=AmpR promoter
CDS 4380..5240
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5411..5999
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatcttacc ctatctgtac agttgttgtg gatggtttgc catctgaaag
781 ctcctcaagt tcttatccag gccctgtgtc tgtttctgaa atgtctctgc ttcatgcttt
841 gggtccagtg cagacctggc tgggacaaga gctcgagaaa tgtggcattg atgccatgat
901 ttacactcgg tatgtcctca gtcttctgct gcatgacagc tatgactacg acctgcagga
961 acaggagaat gacatcttcc tgggctggga aaaaggagct tataagaaat ggggaaagag
1021 taagaaaaaa tgttcagatc taactctaga agaaatgaaa aaacaggctg ctgtccagtg
1081 tcttcgatct gcttctgatg aaagctctgg tatcgagact ttagtggagg agctctgctc
1141 cagactgaaa gaccttcaga gtaagcaaga agagaagatt cacaaaaagt tagaggggtc
1201 tccctctcca gaggcagaat tatcccctcc agcaaaggat caagtggaaa tgtactatga
1261 agcatttcca ccactttctg agaaaccagt ttgcctgcaa gaaatcatga ctgtgtggaa
1321 caagtctaaa gtctgttctt actctagctc ttcttcatca tccacagccc caccagctag
1381 cacagatact tcctctccta aggactgcaa cagtgaaagt gaagtcacca aggaaagaag
1441 cagtgaagta cccaccactg tgcatgagaa aacccagagc aaaagcaaaa acgagaagga
1501 aaacaaattt agtaatggca caattgaaga aaagcctgct ttgtacaaaa agcaaatccg
1561 acataaacct gaaggaaaga ttcgccctcg ctcgtggtct tctggctcca gtgaagcagg
1621 ctcaagttcc agtgggaatc agggagaatt aaaagcatcc atgaagtatg ttaaagtaag
1681 acacaaggca cgagagattc gaaacaaaaa agggcggaat gggcaaagca ggctttcttt
1741 gaagcacggt gaaaaggctg aaaggaacat tcatactgga agtagtagca gtagcagcag
1801 tggttctgtc aaacagctgt gcaagcgggg taagagacct ttaaaagaaa tagggagaaa
1861 agatcctggg agcactgaag gaaaagacct gtacatggag aatagaaagg acacagagta
1921 taaagaggag cccttgtggt acaccgagcc aattgctgaa tattttgttc ctctgagcag
1981 aaaaagtaaa ctagagacca cataccgaaa cagacaggat acaagtgatc tgacatcaga
2041 ggcagtggaa gaattgtctg aatcagtgca tggtctttgt atcagcaaca ataatcttca
2101 taaaacatac ctcgcagcag gtactttcat tgatggtcat tttgtagaaa tgcctgcagt
2161 tataaatgag gatattgacc tcactgggac ctcattatgt tctctaccag aggacaataa
2221 atacctggat gatattcatc tatcagaatt aacgcacttc tatgaagtgg atattgatca
2281 atccatgttg gatcctggtg cctcagaaac aatgcaagga gaaagtcgga ttttgaatat
2341 gattcgacag aaaagcaaag agaacacaga ttttgaggca gaatgttgca tagtgttaga
2401 tggtatggag ttgcaagggg aacgtgcaat atggacagat tctaccagct ccgtaggtgc
2461 tgagggctta ttcctgcagg accttggcaa tctggctcag ttttgggagt gctgttcatc
2521 cagctccggt gatgctgatg gggagagttt tggaggagac tctccagtta gactctctcc
2581 catcttagac agcacagtgc tcaattcaca cctgcttgct ggcaatcaag agctcttttc
2641 agatattaat gaaggatctg gtataaactc ttgtttttca gtgtttgaag tgcaatgcag
2701 taattctgtt ttaccatttt cttttgaaac actcaacttg ggaaatgaaa atacagattc
2761 tagtgctaat atgcttggga aaacacagtc tagattgcta atatggacca aaaatagtgc
2821 ctttgaagaa aatgaacact gttctaatct ttcaacaaga acttgtagtc catggtccca
2881 ttcagaagaa acacgttcag acaatgaaac attaaatatt cagtttgaag aatccacaca
2941 gtttaatgcc gaagatatta attatgtagt tcctagagtc tcgtcaaatt atgtagatga
3001 agaacttcta gattttttgc aagatgaaac ttgccagcaa aacagtagaa ctttaggtga
3061 gattcctaca ttagttttca aaaaaacatc taaactagaa tccgtctgtg gtattcagct
3121 agaacaaaaa acagaaaaca aaaattttga aactacacaa gtatgtaatg aaagtccaca
3181 tggagatggc tacagctcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
3241 aagctattat tcgtatagga tactttatac gaagttatta agttatggag accggtgtgg
3301 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
3361 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
3421 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
3481 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
3601 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
3661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
3721 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
3841 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
3901 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
3961 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
4021 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
4081 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
4141 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
4201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
4261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
4321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
4381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
4441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
4501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
4561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
4621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
4681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
4741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
4801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
4861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
4921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
4981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
5041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
5101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
5161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
5221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
5281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
5341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
5401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
5461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
5521 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
5581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
5641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
5701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
5761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
5821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
5881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
5941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
6001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
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pAAVLINK-EF1a-HA-5'-KIAA0232质粒载体应用举例
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