| 质粒名称: | pAAVLINK-EF1a-HA-5'-KDM6B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001389 |
| 产品用途: | 5' AAVLINK plasmid for KDM6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-KDM6B载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KDM6B质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KDM6B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KDM6B质粒载体简介
pAAVLINK-EF1a-HA-5'-KDM6B质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KDM6B 6582 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6582)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6582
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3744
/codon_start=1
/label=KDM6B
/translation="HRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGR
CSASIGQPPLPAPLPPSHGSSSGHPSKPYYAPGAPTPRPLHGKLESLHGCVQALLREPA
QPGLWEQLGQLYESEHDSEEATRCYHSALRYGGSFAELGPRIGRLQQAQLWNFHTGSCQ
HRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGL
SPGGKRRRGCNSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPG
LWSTLHGDAWGPERKGSAPPERQEQRHSLPHPYPYPAPAYTAHPPGHRLVPAAPPGPGP
RPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTT
SSSSSSSSNTGLRGVEPNPGIPGADHYQTPALEVSHHGRLGPSAHSSRKPFLGAPAATP
HLSLPPGPSSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGTEGPPRPAP
PPLPHREGFLGPPASRFSVGTQDSHTPPTPPTPTTSSSNSNSGSHSSSPAGPVSFPPPP
YLARSIDPLPRPPSPAQNPQDPPLVPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSF
PKTPEVGPGPPPGPLSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPD
GLANIMKMLDESIRKEEEQQQHEAGVAPQPPLKEPFASLQSPFPTDTAPTTTAPAVAVT
TTTTTTTTTTATQEEEKKPPPALPPPPPLAKFPPPSQPQPPPPPPPSPASLLKSLASVL
EGQKYCYRGTGAAVSTRPGPLPTTQYSPGPPSGATALPPTSAAPSAQGSPQPSASSSSQ
FSTSGGPWARERRAGEEPVPGPMTPTQPPPPLSLPPARSESEVLEEISRACETLVERVG
RSATDPADPVDTAEPADSGTERLLPPAQAKEEAGGVAAVSGSCKRRQKEHQKEHRRHRR
ACKDSVGRRPREG"
misc_feature 839..844
/label=SDS
misc_feature 1104..1109
/label=SDS
CDS complement(1128..1139)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 1361..1366
/label=SDS
misc_feature 1392..1397
/label=SDS
CDS 1488..1517
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 1491..1520
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 1494..1523
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 1525..1530
/codon_start=1
/label=KDM6B
/translation="P"
misc_feature 2567..2572
/label=SDS
misc_feature 2574..2579
/label=SDS
CDS 2992..3021
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 2995..3024
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
misc_feature 3615..3620
/label=SDS
misc_feature 3618..3623
/label=SDS
intron 3745..3785
/label=Splicing Donor
misc_feature 3789..3822
/label=lox2272-JT1571
repeat_region 3867..3985
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3867..3974
/label=AAV2 ITR
rep_origin 4060..4515
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4797..4901
/gene="bla"
/label=AmpR promoter
CDS 4902..5762
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5933..6521
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctcatc gggcagtgga ccctccaggg gcccgcgctg cacgggaagc
781 ctttgccctt gggggcctga gctgtgctgg ggcctggagc tcctgcccgc ctcatccccc
841 tcctcgtagc gcatggctgc ctggaggcag atgctcagcc agcattgggc agcccccgct
901 tcctgctccc ctaccccctt cacatggcag tagttctggg caccccagca aaccatatta
961 tgctccaggg gcgcccactc caagacccct ccatgggaag ctggaatccc tgcatggctg
1021 tgtgcaggca ttgctccggg agccagccca gccagggctt tgggaacagc ttgggcaact
1081 gtacgagtca gagcacgata gtgaggaggc cacacgctgc taccacagcg cccttcgata
1141 cggaggaagc ttcgctgagc tggggccccg cattggccga ctgcagcagg cccagctctg
1201 gaactttcat actggctcct gccagcaccg agccaaggtc ctgcccccac tggagcaagt
1261 gtggaacttg ctacaccttg agcacaaacg gaactatgga gccaagcggg gaggtccccc
1321 ggtgaagcga gctgctgaac ccccagtggt gcagcctgtg cctcctgcag cactctcagg
1381 cccctcaggg gaggagggcc tcagccctgg aggcaagcga aggagaggct gcaactctga
1441 acagactggc cttcccccag ggctgccact gcctccacca ccattaccac caccaccacc
1501 accaccacca ccaccaccac caccaccacc cctgcctggc ctggctacca gccccccatt
1561 tcagctaacc aagccagggc tgtggagtac cctgcatgga gatgcctggg gcccagagcg
1621 caagggttca gcacccccag agcgccagga gcagcggcac tcgctgcctc acccatatcc
1681 atacccagct ccagcgtaca ccgcgcaccc ccctggccac cggctggtcc cggctgctcc
1741 cccaggccca ggcccccgcc ccccaggagc agagagccat ggctgcctgc ctgccacccg
1801 tccccccgga agtgacctta gagagagcag agttcagagg tcgcggatgg actccagcgt
1861 ttcaccagca gcaaccaccg cctgcgtgcc ttacgcccct tcccgccccc ctggcctccc
1921 cggcaccacc accagcagca gcagtagcag cagcagcaac actggtctcc ggggcgtgga
1981 gccgaaccca ggcattcccg gcgctgacca ttaccaaact cccgcgctgg aggtctctca
2041 ccatggccgc ctggggccct cggcacacag cagtcggaaa ccgttcttgg gggctcccgc
2101 tgccactccc cacctatccc tgccacctgg accttcctca ccccctccac ccccctgtcc
2161 ccgcctctta cgccccccac caccccctgc ctggttgaag ggtccggcct gccgggcagc
2221 ccgagaggat ggagagatct tagaagagct cttctttggg actgagggac ccccccgccc
2281 tgccccacca cccctccccc atcgcgaggg cttcttgggg cctccggcct cccgcttttc
2341 tgtgggcact caggattctc acacccctcc cactccccca accccaacca ccagcagtag
2401 caacagcaac agtggcagcc acagcagcag ccctgctggg cctgtgtcct ttcccccacc
2461 accctatctg gccagaagta tagaccccct tccccggcct cccagcccag cacagaaccc
2521 ccaggaccca cctcttgtac ccctgactct tgccctgcct ccagcccctc cttcctcctg
2581 ccaccaaaat acctcaggaa gcttcaggcg cccggagagc ccccggccca gggtctcctt
2641 cccaaagacc cccgaggtgg ggccggggcc acccccaggc cccctgagta aagcccccca
2701 gcctgtgccg cccggggttg gggagctgcc tgcccgaggc cctcgactct ttgattttcc
2761 ccccactccg ctggaggacc agtttgagga gccagccgaa ttcaagatcc tacctgatgg
2821 gctggccaac atcatgaaga tgctggacga atccattcgc aaggaagagg aacagcaaca
2881 acacgaagca ggcgtggccc cccaaccccc gctgaaggag ccctttgcat ctctgcagtc
2941 tcctttcccc accgacacag cccccaccac tactgctcct gctgtcgccg tcaccaccac
3001 caccaccacc accaccacca ccacggccac ccaggaagag gagaagaagc caccaccagc
3061 cctaccacca ccaccgcctc tagccaagtt ccctccaccc tctcagccac agccaccacc
3121 acccccaccc cccagcccgg ccagcctgct caaatccttg gcctccgtgc tggagggaca
3181 aaagtactgt tatcggggga ctggagcagc tgtttccacc cggcctgggc ccttgcccac
3241 cactcagtat tcccctggcc ccccatcagg tgctaccgcc ctgccgccca cctcagcggc
3301 ccctagcgcc cagggctccc cacagccctc tgcttcctcg tcatctcagt tctctacctc
3361 aggcgggccc tgggcccggg agcgcagggc gggcgaagag ccagtcccgg gccccatgac
3421 ccccacccaa ccgcccccac ccctatctct gccccctgct cgctctgagt ctgaggtgct
3481 agaagagatc agccgggctt gcgagaccct tgtggagcgg gtgggccgga gtgccactga
3541 cccagccgac ccagtggaca cagcagagcc agcggacagt gggactgagc gactgctgcc
3601 ccccgcacag gccaaggagg aggctggcgg ggtggcggca gtgtcaggca gctgtaagcg
3661 gcgacagaag gagcatcaga aggagcatcg gcggcacagg cgggcctgta aggacagtgt
3721 gggtcgtcgg ccccgtgagg gcaggtaagt atcaaggtta caagacaggt ttaaggagac
3781 caataagcta ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt
3841 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
3901 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccggc ctcagtgagc
3961 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
4021 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
4081 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
4141 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
4201 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
4261 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
4321 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
4381 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
4441 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
4501 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
4561 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
4621 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
4681 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
4741 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
4801 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
4861 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
4921 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
4981 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5041 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5101 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5161 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5221 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5281 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5341 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
5401 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
5461 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
5521 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
5581 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
5641 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
5701 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
5761 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
5821 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
5881 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
5941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6061 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
6121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
6481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
6541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
pAAVLINK-EF1a-HA-5'-KDM6B载体图谱质粒图谱pdf版和pAAVLINK-EF1a-HA-5'-KDM6B载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-HA-5'-KDM6B质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG
- 下一篇：没有了
