| 质粒名称: | pAAVLINK-EF1a-HA-5'-KDM5C |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001371 |
| 产品用途: | 5' AAVLINK plasmid for KDM5C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
KDM5C
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-KDM5C载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KDM5C质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KDM5C载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KDM5C质粒载体简介
pAAVLINK-EF1a-HA-5'-KDM5C质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KDM5C 6424 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6424)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6424
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3564
/codon_start=1
/label=KDM5C
/translation="EPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKI
RPPADWQPPFAVEVDNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNV
ERRILDLYSLSKIVVEEGGYEAICKDRRWARVAQRLNYPPGKNIGSLLRSHYERIVYPY
EMYQSGANLVQCNTRPFDNEEKDKEYKPHSIPLRQSVQPSKFNSYGRRAKRLQPDPEPT
EEDIEKNPELKKLQIYGAGPKMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVK
VESTSPKTFLESKEELSHSPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLL
CDGCDDNYHIFCLLPPLPEIPKGVWRCPKCVMAECKRPPEAFGFEQATREYTLQSFGEM
ADSFKADYFNMPVHMVPTELVEKEFWRLVNSIEEDVTVEYGADIHSKEFGSGFPVSDSK
RHLTPEEEEYATSGWNLNVMPVLEQSVLCHINADISGMKVPWLYVGMVFSAFCWHIEDH
WSYSINYLHWGEPKTWYGVPSLAAEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLM
SHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPAGRQCIEHYRRL
RRYCVFSHEELICKMAACPEKLDLNLAAAVHKEMFIMVQEERRLRKALLEKGITEAERE
AFELLPDDERQCIKCKTTCFLSALACYDCPDGLVCLSHINDLCKCSSSRQYLRYRYTLD
ELPAMLHKLKVRAESFDTWANKVRVALEVEDGRKRSLEELRALESEARERRFPNSELLQ
QLKNCLSEAEACVSRALGLVSGQEAGPHRVAGLQMTLTELRAFLDQMNNLPCAMHQIGD
VKGVLEQVEAYQAEAREALASLPSSPGLLQSLLERGRQLGVEVPEAQQLQRQVEQARWL
DEVKRTLAPSAR"
misc_feature 1026..1031
/label=SDS
misc_feature 1518..1523
/label=SDS
misc_feature 1554..1559
/label=SDS
misc_feature 1617..1622
/label=SDS
protein_bind 1733..1744
/label=ZF binding site
/bound_moiety="CCR5TC zinc-finger domain"
/note="target sequence for the CCR5TC zinc-finger domain
from the CCR5-224 (+) zinc-finger nuclease (Perez et al.,
2008; Gross et al., 2013)"
misc_feature 1799..1804
/label=SDS
misc_feature 2130..2135
/label=SDS
misc_feature 2419..2424
/label=SDS
intron 3565..3605
/label=Splicing Donor
misc_feature 3609..3642
/label=lox2272-JT1571
repeat_region 3687..3827
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3687..3816
/label=AAV2 ITR
rep_origin 3902..4357
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4639..4743
/gene="bla"
/label=AmpR promoter
CDS 4744..5604
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5775..6363
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgagc cggggtccga cgatttccta ccgccaccgg agtgcccggt
781 gttcgagcct agctgggccg agttccgaga ccctcttggc tacatcgcga aaatcaggcc
841 catcgcagag aaatcgggca tttgcaagat ccgcccaccc gcggactggc agccaccctt
901 tgctgtggaa gtggacaact tcaggtttac cccccgaatc cagaggctga atgagctaga
961 ggcccagacg agagtgaaac tgaactactt ggaccagatt gccaaattct gggaaatcca
1021 gggctcctcc ttaaagattc ccaatgtaga acggcggatc ttggacctct acagtctcag
1081 caaaattgtg gtggaggaag gtggttatga agctatctgc aaggaccgtc ggtgggctcg
1141 ggtagcccag cgcctcaact atccaccagg caaaaatatt ggctccttgc tacgctccca
1201 ctacgaacgc attgtttatc cctatgaaat gtaccagtct ggagccaacc ttgtgcagtg
1261 taacacacgt ccatttgata atgaggagaa ggacaaggaa tacaaacccc acagcatccc
1321 cctacgacag tctgtgcagc cttccaagtt caacagctat ggccggcggg ccaagagact
1381 gcagcctgat ccggaaccca cagaggaaga cattgagaag aatccagagc tgaaaaagct
1441 acagatctat ggggcaggcc ccaagatgat gggcctgggc ctcatggcca aagacaagac
1501 tctgcggaag aaagataagg aggggcctga gtgtcccccc acagtagtgg tgaaggagga
1561 gttaggtggg gatgtgaagg tggagtcaac atcgcctaag accttcctgg agagcaagga
1621 ggagctgagt cacagcccag aaccctgcac caagatgacc atgaggctac ggaggaacca
1681 cagcaatgcc cagtttattg agtcatatgt ctgccggatg tgttctcgag gggatgagga
1741 tgacaagctc ctgctgtgtg atggctgtga tgacaactac cacatcttct gcctgctgcc
1801 tcctctgcct gagatcccca agggtgtctg gcggtgccca aagtgtgtca tggcggagtg
1861 taagcggccc ccagaagcct ttggctttga gcaggctacc cgggaataca ctctgcagag
1921 ctttggcgag atggccgact cctttaaagc tgactacttc aacatgcccg tgcatatggt
1981 gcccacagaa cttgtggaga aggagttctg gaggctggta aatagcattg aggaagatgt
2041 gactgttgag tatggagctg acatccattc caaagaattt ggcagcggtt tccctgtcag
2101 tgacagtaaa cggcacctaa cccccgaaga ggaggagtat gctaccagtg gttggaacct
2161 aaatgtgatg ccggtgttgg aacagtctgt actgtgccac atcaatgcag atatctctgg
2221 catgaaggtg ccctggctct acgtgggcat ggtcttctca gccttttgct ggcatattga
2281 ggatcactgg agttactcca ttaactacct ccactggggt gagccgaaga cctggtatgg
2341 ggtgccctca cttgcagcag aacatttgga agaagtgatg aagaagctga cacctgaact
2401 atttgatagc cagcctgacc tcctgcacca acttgtcacc ctcatgaatc ccaacaccct
2461 catgtcccat ggtgtgccag ttgtccgcac aaaccagtgt gcaggagagt ttgtcatcac
2521 cttcccccgt gcttaccaca gcggcttcaa ccaaggctac aactttgccg aggctgtcaa
2581 cttttgcact gctgactggt tgcctgctgg gcgccagtgc attgagcact accgccggct
2641 ccggagatac tgcgtcttct cccatgagga gcttatctgc aagatggctg cctgcccaga
2701 gaagctagac ctgaacctgg cggcagctgt gcataaggag atgttcatca tggtgcaaga
2761 agagcggcgt ctacgaaagg ccctgctgga gaagggtatc acagaggctg agcgagaggc
2821 tttcgagctg ctcccagatg atgagcgcca gtgtatcaag tgcaagacta cgtgtttcct
2881 gtcagccctg gcctgctacg actgcccaga cggccttgtc tgcctttccc acatcaatga
2941 tctctgcaag tgctccagta gccggcagta cctgcggtat cggtatacct tggatgagct
3001 tcctgccatg ctgcataagc tgaaggttcg ggctgagtcc tttgacacct gggccaacaa
3061 agtgcgagtg gccctggagg tggaggatgg gcggaagcgc agccttgaag aactgagggc
3121 actagagtct gaagcccgtg agcggaggtt tcctaatagt gagctgctgc agcaactaaa
3181 gaactgcctg agtgaggcag aggcttgcgt gtcccgagct ctgggactgg tcagcggcca
3241 ggaagctggc ccccacaggg tggctggtct acagatgacc ctgactgagc tccgggcctt
3301 tctggaccag atgaacaacc tgccttgcgc catgcaccag attggggatg tcaagggtgt
3361 tctggaacag gtggaggcct accaggctga ggctcgtgag gccctggcct cactgccctc
3421 cagtccaggg ctactgcagt ccctgttgga gagggggcgg cagctggggg tggaggtgcc
3481 tgaggcccag cagctccagc ggcaggtgga acaggcgcga tggctggatg aggtgaaacg
3541 cacactggcc ccctcagccc gaaggtaagt atcaaggtta caagacaggt ttaaggagac
3601 caataagcta ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt
3661 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
3721 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
3781 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
3841 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
3901 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
3961 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
4021 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
4081 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
4141 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
4201 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
4261 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
4321 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
4381 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
4441 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
4501 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
4561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
4621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
4681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
4741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
4801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
4861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
4921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
4981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
5041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
5101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
5161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
5221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
5281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
5341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
5401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
5461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
5521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
5581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
5641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
5701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
5761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
5821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
5881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
5941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
6001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
6181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
6241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
6301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
6361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
6421 atgt
//
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