| 质粒名称: |
pAAVLINK-EF1a-HA-5'-KDM3B
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001404 |
| 产品用途: | 5' AAVLINK plasmid for KDM3B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM3B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-KDM3B载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KDM3B质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KDM3B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KDM3B质粒载体简介
pAAVLINK-EF1a-HA-5'-KDM3B质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KDM3B 6537 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6537)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6537
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3677
/codon_start=1
/label=KDM3B
/translation="MADAAASPVGKRLLLLFADTAASASASAPAAAAASGDPGPALRTR
AWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPREDP
VLLQGIRVSIAQWPALTFTPLVDKLGLGSVVPVEYLLDRELRFLSDANGLHLFQMGTDS
QNQILLEHAALRETVNALISDQKLQEIFSRGPYSVQGHRVKIYQPEGEEGWLYGVVSHQ
DSITRLMEVSVTESGEIKSVDPRLIHVMLMDNSTPQSEGGTLKAVKSSKGKKKRESIEG
KDGRRRKSASDSGCDPASKKLKGDRGEVDSNGSDGGEASRGPWKGGNASGEPGLDQRAK
QPPSTFVPQINRNIRFATYTKENGRTLVVQDEPVGGDTPASFTPYSTATGQTPLAPEVG
GAENKEAGKTLEQVGQGIVASAAVVTTASSTPNTVRISDTGLAAGTVPEKQKGSRSQAS
GENSRNSILASSGFGAPLPSSSQPLTFGSGRSQSNGVLATENKPLGFSFGCSSAQEAQK
DTDLSKNLFFQCMSQTLPTSNYFTTVSESLADDSSSRDSFKQSLESLSSGLCKGRSVLG
TDTKPGSKAGSSVDRKVPAESMPTLTPAFPRSLLNARTPENHENLFLQPPKLSREEPSN
PFLAFVEKVEHSPFSSFASQASGSSSSATTVTSKVAPSWPESHSSADSASLAKKKPLFI
TTDSSKLVSGVLGSALTSGGPSLSAMGNGRSSSPTSSLTQPIEMPTLSSSPTEERPTVG
PGQQDNPLLKTFSNVFGRHSGGFLSSPADFSQENKAPFEAVKRFSLDERSLACRQDSDS
STNSDLSDLSDSEEQLQAKTGLKGIPEHLMGKLGPNGERSAELLLGKSKGKQAPKGRPR
TAPLKVGQSVLKDVSKVKKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQ
DDSTVACRFFHFRRLIFTRKGVLRVEGFLSPQQSDPDAMNLWIPSSSLAE"
misc_feature 1592..1597
/label=SDS
misc_feature 1711..1716
/label=SDS
misc_feature 2560..2565
/label=SDS
misc_feature 3016..3021
/label=SDS
intron 3678..3718
/label=Splicing Donor
misc_feature 3722..3755
/label=lox2272-JT1571
repeat_region 3800..3940
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3800..3929
/label=AAV2 ITR
rep_origin 4015..4470
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4752..4856
/gene="bla"
/label=AmpR promoter
CDS 4857..5717
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5888..6476
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctatgg cggacgcggc ggcctccccg gtgggcaagc ggctgctgct
781 gctgttcgcg gacactgcgg cctcagcctc ggcctcggct cccgcggcgg cagcggcgag
841 cggagatccg gggcctgcgc tgcgcactcg agcctggcgg gccggcacgg tgcgggccat
901 gagcggggcg gtgccccagg acctagcgat ctttgtagaa tttgatggct gtaactggaa
961 gcaacactcc tgggtaaaag ttcatgctga ggaagttatt gtgcttctgc tggaagggtc
1021 tcttgtatgg gcgccccgtg aggacccagt ccttctccag ggcattcgag tctccattgc
1081 acaatggcca gccctgactt ttactcccct tgtagataaa ctgggtttgg gttctgtggt
1141 tccagtggaa tatcttctgg atcgagagct tcggttcctg tcagatgcca atgggttgca
1201 tctgtttcag atgggaacag atagccaaaa ccagattctt ttggaacatg ctgcactgag
1261 agaaacagtt aatgctttga tcagtgacca aaagctacaa gagatattca gccgaggtcc
1321 ctacagtgtt caaggtcaca gggtcaaaat atatcagcca gagggggaag aagggtggct
1381 ctatggtgtt gtgagccatc aggactccat cactcgtctt atggaggtgt ctgtaactga
1441 gagtggtgag atcaagtcgg tagatcccag actaatccat gtgatgctga tggataattc
1501 aacgcctcaa agcgaggggg gtacgttaaa agcagtaaaa tcttccaaag gaaagaagaa
1561 gagagaaagc atagagggga aagatggccg gaggaggaaa agtgcttcgg actctgggtg
1621 tgaccctgca tcaaagaaat taaaaggaga caggggtgaa gtagacagta atgggagcga
1681 tggaggtgag gcaagccgag ggccctggaa aggagggaat gccagtggag agccagggct
1741 ggatcagaga gccaagcagc caccgtctac atttgtcccc cagataaacc gcaacattcg
1801 ctttgccact tacaccaaag aaaacggcag gactctggtg gtgcaggatg agcctgtagg
1861 tggggacaca cctgcatctt tcactccata ttctacagcc acaggtcaga cacctttggc
1921 cccagaggtg ggtggagccg aaaacaaaga ggcaggaaaa acactggaac aagttggcca
1981 gggcatagtg gcttccgcag ctgtggtcac taccgccagc tccaccccaa acacagtgag
2041 gatctcagac actggccttg cagcagggac tgtgccagaa aaacagaaag gcagccggtc
2101 gcaggcctcg ggagagaatt caagaaattc tattctggcc tcttctggat ttggagcacc
2161 tctccctagt tcatcgcaac ctttgacttt tggaagtgga aggagccagt ccaatggtgt
2221 tctagccaca gagaacaaac ctttgggctt ctcttttggc tgtagctctg cacaagaggc
2281 acagaaagac actgatctct ccaaaaactt gttttttcaa tgcatgtccc aaactttacc
2341 taccagtaac tacttcacta ctgtttcaga gagtttggct gatgattctt ctagtcggga
2401 ctcattcaaa caaagccttg agagcctgag ctcaggcctg tgtaaaggca gatccgttct
2461 tggaacagac actaagccag gctctaaggc tggcagctct gtggaccgga aagtgcctgc
2521 agagtccatg cccaccctca ctccagcctt cccacggagc ctcctaaatg cccgtacccc
2581 agagaatcat gaaaatctat ttttacagcc ccccaaattg tcccgagaag agccttctaa
2641 tcctttcctg gcatttgtgg agaaagttga acacagccct ttcagtagtt ttgcatctca
2701 ggcatcaggt agctcctctt ctgctaccac tgtcacctcc aaggtggcac ccagctggcc
2761 cgagtctcac tcctctgcag attcggcatc tttagcaaag aagaaacccc tcttcattac
2821 aactgactcc tccaagctag tatctggtgt tctgggctca gctcttacca gtgggggccc
2881 aagcctctct gccatgggga atggccgctc cagctcgccc accagcagcc tcactcagcc
2941 cattgagatg ccaactctct cctctagccc cacagaggag aggccaactg tggggcctgg
3001 gcagcaggac aatcccctcc tcaaaacctt tagtaacgtc tttggcaggc actcaggcgg
3061 ctttctgtcc tccccggcag atttttcaca ggagaacaaa gctccttttg aagctgtgaa
3121 aaggttctca ctggatgaac gaagcttggc ttgcagacaa gactcggact ccagcaccaa
3181 cagtgacctg tcagatttga gtgactctga ggagcagctg caggctaaga caggcctgaa
3241 gggaattcca gagcacctga tggggaagct gggccccaat ggggagcgca gtgctgagct
3301 gttgctgggc aaaagcaaag ggaagcaggc ccccaagggc cggcctcgga ctgcccccct
3361 gaaagttggc cagtcagtgc tgaaagatgt aagcaaagtg aagaagctga agcaatctgg
3421 agagcccttc ctgcaggatg ggtcatgcat caatgtggca cctcatctgc acaagtgtcg
3481 tgaatgccgc ctggagcggt accggaagtt taaggaacag gagcaagatg attctactgt
3541 agcctgccgt ttctttcact tccggaggtt gatcttcact cgaaaagggg tgctccgtgt
3601 ggaggggttt ttaagccccc agcaaagtga ccctgatgcc atgaacctgt ggattccctc
3661 ttcctcccta gcagaaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag
3721 ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac
3781 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
3841 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
3901 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
3961 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
4021 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
4081 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
4141 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
4201 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
4261 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
4321 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
4381 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
4441 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
4501 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
4561 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4621 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
4681 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
4741 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
4801 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
4861 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
4921 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
4981 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
5041 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
5101 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
5161 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
5221 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
5281 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
5341 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
5401 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
5461 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
5521 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
5581 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
5641 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
5701 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
5761 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
5821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
5881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
5941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
6001 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
6061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
6121 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
6181 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
6241 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
6301 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
6361 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
6421 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
6481 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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