| 质粒名称: | pAAVLINK-EF1a-HA-5'-KDM2B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001350 |
| 产品用途: | 5' AAVLINK plasmid for KDM2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-KDM2B载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KDM2B质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KDM2B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KDM2B质粒载体简介
pAAVLINK-EF1a-HA-5'-KDM2B质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KDM2B 5871 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5871)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5871
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3011
/codon_start=1
/gene="KDM2B"
/gene_synonym="CXXC2; Fbl10; FBXL10; JHDM1B; PCCX2"
/product="lysine-specific demethylase 2B isoform a"
/EC_number="1.14.11.27"
/label=KDM2B
/note="isoform a is encoded by transcript variant 1;jumonji
C domain-containing histone demethylase 1B; F-box and
leucine-rich repeat protein 10; JEMMA (Jumonji domain,
EMSY-interactor, methyltransferase motif) protein; F-box
protein FBL10; lysine (K)-specific demethylase 2B;
F-box/LRR-repeat protein 10; CXXC-type zinc finger protein
2; protein-containing CXXC domain 2; [Histone-H3]-lysine-36
demethylase 1B; jmjC domain-containing histone
demethylation protein 1B"
/db_xref="GI:54112382"
/db_xref="CCDS:CCDS41850.1"
/db_xref="GeneID:84678"
/db_xref="HGNC:HGNC:13610"
/db_xref="MIM:609078"
/protein_id="NP_115979.3"
/translation="AGPQMGGSAEDHPPRKRHAAEKQKKKTVIYTKCFEFESATQRPID
RQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREALRVP
LIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPE
AQRDKLYNVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNAIAEMKY
PKVKKYCLMSVKGCFTDFHIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGK
QSDIFLGDRVERCQRIELKQGYTFFIPSGWIHAVYTPVDSLVFGGNILHSFNVPMQLRI
YEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSHLTQEYQRESMLIDAPRKPSID
GFSSDSWLEMEEEACDQQPQEEEEKDEEGEGRDRAPKPPTDGSTSPTSTPSEDQEALGK
KPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLK
ALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHADDDPSLAITGVPVVTWPKKTP
KNRAVGRPKGKLGPASAVKLVANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKF
GGPGRMKQSCIMRQCIAPVLPHTAVCLVCGEAGKEDTVEEEEGKFNLMLMECSICNEII
HPGCLKIKESEGVVNDELPNCWECPKCNHAGKTGKQKRGPGFKYASNLPGSLLKEQKMN
RDNKE"
misc_feature 1965..1970
/label=SDS
misc_feature 1968..1973
/label=SDS
misc_feature 1992..1997
/label=SDS
misc_feature 1995..2000
/label=SDS
misc_feature 1998..2003
/label=SDS
misc_feature 2013..2018
/label=SDS
misc_feature 2094..2099
/label=SDS
misc_feature 2760..2765
/label=SDS
intron 3012..3052
/label=Splicing Donor
misc_feature 3056..3089
/label=lox2272-JT1571
repeat_region 3134..3274
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3134..3263
/label=AAV2 ITR
rep_origin 3349..3804
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4086..4190
/gene="bla"
/label=AmpR promoter
CDS 4191..5051
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5222..5810
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgcgg gtccgcaaat ggggggatct gcagaggatc accccccacg
781 aaaaagacat gcagcagaaa agcaaaaaaa gaaaacagtt atatatacaa aatgctttga
841 atttgagtcg gccacacagc gcccgattga ccgccagcga tacgacgaga acgaggactt
901 gtcggacgtg gaggagatcg tcagcgtccg cggcttcagc ctggaggaga agcttcgcag
961 ccagctgtac cagggggact tcgtgcacgc catggagggc aaagatttca actatgagta
1021 cgtacagaga gaagctctca gggttcccct gatatttcga gaaaaggatg gactgggaat
1081 taagatgcct gaccctgatt tcacagtccg agacgtcaaa ctcctagtgg ggagccggcg
1141 gcttgtggac gtgatggatg tgaacaccca gaagggcacg gagatgagca tgtcccagtt
1201 tgtgcgttac tacgagacgc ccgaggccca gcgggacaag ctgtacaacg tcatcagcct
1261 agagttcagc cacaccaagc tggagcactt ggtcaagcgt ccgactgtgg tagacctggt
1321 ggactgggtg gacaacatgt ggccccagca tctgaaggag aagcagacag aagccacgaa
1381 cgccattgca gagatgaagt acccgaaagt gaaaaagtac tgtctgatga gcgtgaaagg
1441 ttgtttcacc gacttccaca tcgactttgg aggcacttcc gtttggtacc atgttttccg
1501 gggtgggaag attttttggc tgattcctcc aacgctgcac aatttggcgc tgtacgagga
1561 gtgggtgctg tcaggcaaac agagtgacat ctttctggga gaccgtgtgg aacgatgcca
1621 aagaattgag ctgaagcagg gctacacatt tttcatccct tccggttgga tccatgccgt
1681 ctacacccct gtagactctt tggtgttcgg cggaaacatc ctgcacagct ttaacgtgcc
1741 catgcagctg cggatctacg agatcgagga caggacgcgg gtgcagccca aattccgtta
1801 ccccttctac tatgagatgt gctggtatgt cctggagaga tacgtgtact gtgtgaccca
1861 gcgctcccac ctcactcagg aataccagag ggagtcgatg cttattgatg ccccgaggaa
1921 gcccagcata gacggcttct cttcggattc ctggctggag atggaggagg aggcctgtga
1981 tcagcagcct caggaggagg aggagaagga cgaggagggc gagggcaggg acagggcacc
2041 caaaccgccc accgatggct ccacttcacc caccagcacg ccctctgagg accaggaggc
2101 cctcgggaag aagcccaaag cacctgccct gcgattcctc aaaaggactt tgtctaatga
2161 gtcggaggaa agtgtgaagt ccaccacatt ggccgtagac taccccaaga cccccaccgg
2221 ctctcccgcc acggaggtct ctgccaaatg gacccatctc actgagtttg aactgaaggg
2281 cctgaaagct ctggtggaga aactggaatc cctcccggag aacaagaagt gtgtccccga
2341 gggcatcgag gacccccagg cactcctgga gggtgtgaag aacgtcctga aggagcacgc
2401 agatgatgac cctagtctgg ccatcactgg ggtccctgtg gtgacttggc caaagaagac
2461 tccaaagaac cgggctgtgg gtcggcccaa ggggaagctg ggcccggcct ccgcggtgaa
2521 gttggtcgcc aaccggacaa cggcaggagc tcggcggcgc cggacgcgat gccgcaagtg
2581 cgaggcctgc ctgcggaccg agtgcggaga gtgccacttc tgcaaggaca tgaagaagtt
2641 cgggggcccc gggcgcatga agcagagctg catcatgcgg cagtgcatcg cgccagtgct
2701 gccccacacc gccgtgtgcc ttgtgtgtgg cgaggcgggg aaggaagaca cggtggaaga
2761 ggaggaaggc aagtttaacc tcatgctcat ggagtgctcc atctgcaatg aaatcatcca
2821 ccctggatgc cttaagatta aggagtcaga gggtgtggtc aacgacgagc ttccaaactg
2881 ctgggagtgt ccgaagtgta accacgccgg caagaccggg aaacaaaagc gtggccctgg
2941 ctttaagtac gcctccaacc tgcccggctc cctgctcaag gagcagaaga tgaaccggga
3001 caacaaggaa ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta
3061 ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg
3121 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
3181 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
3241 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
3301 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
3361 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
3421 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
3481 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
3541 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
3601 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
3661 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
3721 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
3781 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
3841 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
3901 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
3961 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
4021 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
4081 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
4141 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
4201 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
4261 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
4321 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
4381 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
4441 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
4501 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
4561 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
4621 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
4681 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
4741 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
4801 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
4861 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
4921 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
4981 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
5041 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
5101 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
5161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
5221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
5281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
5341 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
5401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
5461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
5521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
5581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
5641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
5701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
5761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
5821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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