| 质粒名称: | pAAVLINK-EF1a-HA-5'-KANK1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001524 |
| 产品用途: | 5' AAVLINK plasmid for KANK1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KANK1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-KANK1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-KANK1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KANK1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KANK1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KANK1质粒载体简介
pAAVLINK-EF1a-HA-5'-KANK1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KANK1 5630 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5630)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5630
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..2770
/codon_start=1
/label=KANK1
/translation="AHTTKVNGSASGKAGDILSGDQDKEQKDPYFVETPYGYQLDLDFL
KYVDDIQKGNTIKRLNIQKRRKPSVPCPEPRTTSGQQGIWTSTESLSSSNSDDNKQCPN
FLIARSQVTSTPISKPPPPLETSLPFLTIPENRQLPPPSPQLPKHNLHVTKTLMETRRR
LEQERATMQMTPGEFRRPRLASFGGMGTTSSLPSFVGSGNHNPAKHQLQNGYQGNGDYG
SYAPAAPTTSSMGSSIRHSPLSSGISTPVTNVSPMHLQHIREQMAIALKRLKELEEQVR
TIPVLQVKISVLQEEKRQLVSQLKNQRAASQINVCGVRKRSYSAGNASQLEQLSRARRS
GGELYIDYEEEEMETVEQSTQRIKEFRQLTADMQALEQKIQDSSCEASSELRENGECRS
VAVGAEENMNDIVVYHRGSRSCKDAAVGTLVEMRNCGVSVTEAMLGVMTEADKEIELQQ
QTIEALKEKIYRLEVQLRETTHDREMTKLKQELQAAGSRKKVDKATMAQPLVFSKVVEA
VVQTRDQMVGSHMDLVDTCVGTSVETNSVGISCQPECKNKVVGPELPMNWWIVKERVEM
HDRCAGRSVEMCDKSVSVEVSVCETGSNTEESVNDLTLLKTNLNLKEVRSIGCGDCSVD
VTVCSPKECASRGVNTEAVSQVEAAVMAVPRTADQDTSTDLEQ"
misc_feature 1782..1787
/label=SDS
misc_feature 1896..1901
/label=SDS
intron 2771..2811
/label=Splicing Donor
misc_feature 2815..2848
/label=lox2272-JT1571
repeat_region 2893..3033
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2893..3022
/label=AAV2 ITR
rep_origin 3108..3563
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3845..3949
/gene="bla"
/label=AmpR promoter
CDS 3950..4810
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4981..5569
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgctc acaccacaaa ggttaacggc agtgcctcag gaaaagcagg
781 tgatattctc agtggagacc aggacaagga acagaaagac ccttactttg tggagacccc
841 ctatggttat caactagact tagatttcct caaatatgtg gatgacatac agaagggaaa
901 taccatcaaa agactgaaca tccagaagag gcggaagccg tccgtgccat gcccagaacc
961 caggaccaca tctggtcagc aaggtatatg gacttccact gaatccctct catcctccaa
1021 cagtgatgac aacaagcagt gccccaactt cctcatagcc agaagtcaag ttacatcaac
1081 tccaatctca aagccacctc cccctctgga gacctcactc ccttttctta ccatcccaga
1141 aaatcgacag ctgccacctc cctcaccaca actcccaaag cataaccttc atgtcaccaa
1201 gacactgatg gagacccgga gaagactgga acaggagaga gccaccatgc agatgacacc
1261 gggtgagttc agaaggccca ggctggccag ttttggaggc atgggcacca caagctccct
1321 cccttctttt gtgggttctg gaaaccacaa tcctgccaag caccagcttc agaatggata
1381 ccaaggtaat ggggattatg gtagctatgc cccagctgct cccaccactt cctccatggg
1441 gagctccatc cgccacagcc ccctgagctc agggatctcc accccagtga ccaacgtgag
1501 ccccatgcac ctgcagcaca tccgcgagca gatggccatt gctctgaaac gcctgaagga
1561 gctggaggag caggtgcgaa ccatccctgt gctccaggta aagatctctg tcttgcaaga
1621 agagaaaagg cagttggtct cacagctgaa aaaccaaagg gctgcatccc agatcaatgt
1681 ctgtggtgtg aggaagcggt cctatagtgc ggggaacgcc tcccagctgg aacagctctc
1741 ccgggcccga agaagtggcg gggaattata cattgactat gaggaggaag aaatggagac
1801 cgtagaacag agcacgcaga ggataaagga gttccggcaa cttacagcag acatgcaagc
1861 cctggagcag aagatccagg acagcagctg tgaggcctcc tcagagctca gggagaatgg
1921 agagtgccgg tctgtggctg tgggtgccga ggagaacatg aacgacatcg tcgtgtacca
1981 cagaggctcc aggtcctgta aggatgcagc tgtagggaca cttgttgaga tgagaaattg
2041 tggggtcagc gtgacagagg ccatgcttgg agtgatgact gaagctgaca aagaaattga
2101 gctccaacag cagaccatag aagccttgaa ggaaaagatc tatcgcctag aagtacagct
2161 tagagaaacc acccatgacc gggagatgac taaactgaaa caagagctgc aggctgctgg
2221 atcgaggaaa aaggttgaca aagccacgat ggcccagccg cttgttttca gtaaggtggt
2281 ggaggcagtg gtgcagacca gagaccaaat ggtcggcagt cacatggacc tggtggacac
2341 gtgtgttggg acctccgtgg aaacaaacag tgtaggcatc tcctgccagc ctgaatgtaa
2401 gaataaagtc gtagggcctg agctgcctat gaattggtgg attgttaagg agagggtgga
2461 aatgcatgac cgatgtgctg ggaggtctgt ggaaatgtgt gacaagagtg tgagtgtgga
2521 agtcagcgtc tgcgaaacag gcagcaacac agaggagtct gtgaacgacc tcacactcct
2581 caagacaaac ttgaatctca aagaagtgcg gtctatcggt tgtggagatt gttctgttga
2641 cgtgaccgtc tgctctccaa aggagtgcgc ctcccggggc gtgaacactg aggctgttag
2701 ccaggtggaa gctgccgtca tggcagtgcc tcgtactgca gaccaggaca ctagcacaga
2761 tttggaacag gtaagtatca aggttacaag acaggtttaa ggagaccaat aagctattat
2821 tcgtatagga tactttatac gaagttatta agttatggag accggtgtgg cacgtgcgga
2881 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
2941 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
3001 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
3061 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
3121 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3181 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
3241 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
3301 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
3361 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3421 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
3481 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
3541 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
3601 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
3661 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
3721 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
3781 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
3841 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
3901 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
3961 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
4021 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
4081 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
4141 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
4201 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
4261 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
4321 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
4381 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
4441 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
4501 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
4561 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
4621 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
4681 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
4741 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
4801 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
4861 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
4921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
4981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
5041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
5101 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
5161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
5221 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
5281 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
5341 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
5401 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
5461 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
5521 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
5581 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
