| 质粒名称: | pAAVLINK-EF1a-HA-5'-DIP2A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001377 |
| 产品用途: | 5' AAVLINK plasmid for DIP2A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DIP2A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-DIP2A载体质粒基本信息
pAAVLINK-EF1a-HA-5'-DIP2A质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-DIP2A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-DIP2A质粒载体简介
pAAVLINK-EF1a-HA-5'-DIP2A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-DIP2A 6351 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6351)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6351
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3491
/codon_start=1
/label=DIP2A
/translation="ADRGCPLEAAPLPAEVRESLAELELELSEGDITQKGYEKKRAKLL
ARYIPLIQGIDPSLQAENRIPGPSQTTAAAPKQQKSRPTASRDERFRSDVHTEAVQAAL
AKYKERKMPMPSKRRSVLVHSSVETYTPPDTSSASEDEGSLRRPGRLTSTPLQSHSSVE
PWLDRVIQGSSTSSSASSTSSHPGGRPTTAPSAAATPGAAATTALAGLEAHTHIDLHSA
PPDVTTGLVEHSYFERPQVASVRSVPRGCSGSMLETADGVPVNSRVSSKIQQLLNTLKR
PKRPPLKEFFVDDFEELLEVQQPDPNQPKPEGSETSVLRGEPLTAGVPRPPSLLATLQR
WGTTQPKSPCLTALDTTGKAVYTLTYGKLWSRSLKLAYTLLNKLTSKNEPLLKPGDRVA
LVFPNSDPVMFMVAFYGCLLAELVPVPIEVPLTRKDAGSQQVGFLLGSCGVFLALTTDA
CQKGLPKAQTGEVAAFKGWPPLSWLVIDGKHLAKPPKDWHPLAQDTGTGTAYIEYKTSK
EGSTVGVTVSHASLLAQCRALTQACGYSEAETLTNVLDFKRDAGLWHGVLTSVMNRMHV
VSVPYALMKANPLSWIQKVCFYKARAALVKSRDMHWSLLAQRGQRDVSLSSLRMLIVAD
GANPWSISSCDAFLNVFQSRGLRPEVICPCASSPEALTVAIRRPPDLGGPPPRKAVLSM
NGLSYGVIRVDTEEKLSVLTVQDVGQVMPGANVCVVKLEGTPYLCKTDEVGEICVSSSA
TGTAYYGLLGITKNVFEAVPVTTGGAPIFDRPFTRTGLLGFIGPDNLVFIVGKLDGLMV
TGVRRHNADDVVATALAVEPMKFVYRGRIAVFSVTVLHDDRIVLVAEQRPDASEEDSFQ
WMSRVLQAIDSIHQVGVYCLALVPANTLPKAPLGGIHISETKQRFLE"
misc_feature 1403..1408
/label=SDS
misc_feature 2664..2669
/label=SDS
misc_feature 3064..3069
/label=SDS
misc_feature 3333..3338
/label=SDS
intron 3492..3532
/label=Splicing Donor
misc_feature 3536..3569
/label=lox2272-JT1571
repeat_region 3614..3754
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3614..3743
/label=AAV2 ITR
rep_origin 3829..4284
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4566..4670
/gene="bla"
/label=AmpR promoter
CDS 4671..5531
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5702..6290
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgctg accgcgggtg cccgctggag gcggcgccgc tgcctgccga
781 ggtgcgggag agcctggctg agctggagct ggagctgtcg gaaggtgaca tcactcaaaa
841 aggatatgaa aagaaaaggg caaagctgct tgcacgttat ataccgctta ttcaaggaat
901 agacccatct ctgcaagcag agaatagaat tcctgggccc tcacaaacca cggccgctgc
961 acccaagcag cagaagtctc ggcccaccgc ctcgagggat gagcgcttcc ggtcagatgt
1021 ccacactgaa gccgtgcaag cagctttggc caaatacaaa gagaggaaga tgcctatgcc
1081 ttcgaagaga cgttctgtcc ttgtgcattc gtctgtggaa acctacaccc ctccagacac
1141 gtcgtctgcc tcagaagatg agggctcttt acggcgaccc gggcgactca cctccactcc
1201 gctccagagc cattccagcg tcgagccctg gctcgaccgg gtcattcagg gctcgtccac
1261 ctcatcctct gcatcctcca cctcatctca cccgggaggg agacccacca ctgctcccag
1321 tgctgcagcc acgccggggg ccgccgctac cactgcactc gcaggcctcg aggcccacac
1381 ccacatagat ctgcattctg cccctcctga tgtcaccacg ggcctcgtgg agcattcgta
1441 ctttgagcgt ccacaggtgg cttctgtgag aagtgttcct cgggggtgca gcgggagcat
1501 gctggaaaca gcagatggtg tccctgtgaa cagcagagtg tcctccaaaa tccagcagct
1561 tctgaacacc ctgaagaggc caaagcgccc tccactgaag gagttctttg tggatgattt
1621 tgaggaattg ttggaagttc agcaaccaga tccaaatcag ccaaagcctg agggaagcga
1681 gacgagtgtg ctgagagggg agcctctcac tgcaggtgtc ccccgaccgc cgtcgctgtt
1741 ggccaccttg cagcgctggg gcacaacaca gcccaaatcc ccctgtctga ctgccttgga
1801 tacaactggg aaagccgtct acactctcac ctatggtaaa ctttggagtc ggagtttaaa
1861 actagcttat actctactta ataaactgac aagtaagaat gaacctctac ttaaacctgg
1921 agacagagtg gcgctcgtgt ttccgaatag tgaccctgtg atgttcatgg ttgcatttta
1981 tgggtgtctc ctggcagagc tggttcctgt ccccatagaa gtgccattaa caagaaagga
2041 tgcaggcagc cagcaggttg ggtttctgct gggcagctgt ggagtcttct tggccctgac
2101 cacagacgct tgtcagaaag gcctccccaa ggcacagaca ggagaggtgg cagctttcaa
2161 aggttggccc ccgctctcct ggctagtgat tgatgggaag catctagcca agcccccaaa
2221 ggactggcac cctctggccc aggacacagg gactgggact gcctacattg agtataaaac
2281 cagcaaagaa ggcagtacgg tgggggtcac agtgtcccac gcatccctgc tggcacagtg
2341 ccgggctctg acccaggcgt gcgggtactc agaagctgaa acattaacaa acgtgctgga
2401 tttcaaaagg gatgctggtc tgtggcatgg cgtgttaaca agcgtcatga acaggatgca
2461 cgtggtcagc gtcccctacg cgctgatgaa ggcgaaccca ctctcctgga tccagaaagt
2521 gtgcttctat aaagctcggg ccgcgctggt gaagtcgcga gacatgcact ggtctctcct
2581 agctcagcgg ggccagaggg acgtcagcct cagctcactg cgcatgctga ttgtggccga
2641 tggtgccaac ccgtggtcga tctcctcctg tgacgccttc ctcaacgtct tccagtccag
2701 aggtctgagg ccagaggtca tctgtccttg tgcaagttct cctgaggcgc tgactgtcgc
2761 catccgcagg ccacctgatc tgggaggacc acctccaaga aaagcagtcc tatcgatgaa
2821 cggtctaagt tatggtgtta tcagagtgga tactgaagaa aagttgtcag tccttactgt
2881 tcaggacgtt ggtcaggtga tgcctggagc taatgtatgt gttgtgaagt tagaaggtac
2941 cccttatctt tgtaaaactg atgaagtggg agaaatatgc gtcagttcca gtgcaactgg
3001 cacagcgtac tatggattgc ttggaatcac gaagaatgtg tttgaggcag ttccggtcac
3061 cacaggagga gcacccatct ttgacaggcc attcaccagg acaggcctgc tgggcttcat
3121 cgggcctgac aacctggtct tcatcgtggg caaactggac gggctgatgg tcactggagt
3181 tcgcagacac aatgcagatg acgttgtggc caccgcactg gccgtggagc ccatgaagtt
3241 tgtctacaga ggcaggatcg ctgtgttctc tgtgaccgtg ctgcacgacg accggattgt
3301 cctggtggct gagcagcggc cggatgcctc ggaggaggac agcttccagt ggatgagccg
3361 tgtgctgcag gccattgata gcatccacca ggtgggcgtg tactgtctgg ccctggttcc
3421 tgccaacacc ttgcccaagg ctcctctcgg agggattcac atttctgaaa ccaaacagcg
3481 ctttctggaa ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta
3541 ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg
3601 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
3661 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
3721 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
3781 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
3841 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
3901 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
3961 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
4021 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
4081 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
4141 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
4201 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
4261 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
4321 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
4381 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
4441 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
4501 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
4561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
4621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
4681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
4741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
4801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
4861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
4921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
4981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
5041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
5101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
5161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
5221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
5281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
5341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
5401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
5461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
5521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
5581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
5641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
5701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
5761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
5821 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
5881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
5941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
6001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
6061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
6121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
6181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
6241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
6301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
pAAVLINK-EF1a-HA-5'-DIP2A载体图谱质粒图谱pdf版和pAAVLINK-EF1a-HA-5'-DIP2A载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-HA-5'-DIP2A质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG
- 下一篇：没有了
