| 质粒名称: | pAAVLINK-EF1a-HA-5'-CUX2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001482 |
| 产品用途: | 5' AAVLINK plasmid for CUX2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CUX2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-CUX2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CUX2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CUX2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CUX2质粒载体简介
pAAVLINK-EF1a-HA-5'-CUX2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CUX2 6129 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6129)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6129
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3269
/codon_start=1
/label=CUX2
/translation="MAANVGSMFQYWKRFDLRRLQKELNSVASELSARQEESEHSHKHL
IELRREFKKNVPEEIREMVAPVLKSFQAEVVALSKRSQEAEAAFLSVYKQLIEAPDPVP
VFEAARSLDDRLQPPSFDPSGQPRRDLHTSWKRNPELLSPKEQREGTSPAGPTLTEGSR
LPGIPGKALLTETLLQRNEAEKQKGLQEVQITLAARLGEAEEKIKVLHSALKATQAELL
ELRRKYDEEAASKADEVGLIMTNLEKANQRAEAAQREVESLREQLASVNSSIRLACCSP
QGPSGDKVNFTLCSGPRLEAALASKDREILRLLKDVQHLQSSLQELEEASANQIADLER
QLTAKSEAIEKLEEKLQAQSDYEEIKTELSILKAMKLASSTCSLPQGMAKPEDSLLIAK
EAFFPTQKFLLEKPSLLASPEEDPSEDDSIKDSLGTEQSYPSPQQLPPPPGPEDPLSPS
PGQPLLGPSLGPDGTRTFSLSPFPSLASGERLMMPPAAFKGEAGGLLVFPPAFYGAKPP
TAPATPAPGPEPLGGPEPADGGGGGAAGPGAEEEQLDTAEIAFQVKEQLLKHNIGQRVF
GHYVLGLSQGSVSEILARPKPWRKLTVKGKEPFIKMKQFLSDEQNVLALRTIQVRQRGS
ITPRIRTPETGSDDAIKSILEQAKKEIESQKGGEPKTSVAPLSIANGTTPASTSEDAIK
SILEQARREMQAQQQALLEMEVAPRGRSVPPSPPERPSLATASQNGAPALVKQEEGSGG
PAQAPLPVLSPAAFVQSIIRKVKSEIGDAGYFDHHWASDRGLLSRPYASVSPSLSSSSS
SGYSGQPNGRAWPRGDEAPVPPEDEAAA"
misc_feature 852..857
/label=SDS
misc_feature 996..1001
/label=SDS
misc_feature 1261..1266
/label=SDS
misc_feature 1437..1442
/label=SDS
misc_feature 1731..1736
/label=SDS
misc_feature 1944..1949
/label=SDS
misc_feature 1990..1995
/label=SDS
misc_feature 2394..2399
/label=SDS
misc_feature 2988..2993
/label=SDS
misc_feature 2991..2996
/label=SDS
misc_feature 3171..3176
/label=SDS
misc_feature 3174..3179
/label=SDS
misc_feature 3177..3182
/label=SDS
misc_feature 3180..3185
/label=SDS
intron 3270..3310
/label=Splicing Donor
misc_feature 3314..3347
/label=lox2272-JT1571
repeat_region 3392..3532
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3392..3521
/label=AAV2 ITR
rep_origin 3607..4062
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4344..4448
/gene="bla"
/label=AmpR promoter
CDS 4449..5309
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5480..6068
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccgccaat gtgggatcga tgtttcaata
781 ttggaagcga tttgatctac ggcgactcca gaaggagctt aattccgtcg cttctgagct
841 gtctgcacgg caggaggaga gtgaacattc tcataaacat ttaattgaac tccgccggga
901 atttaagaaa aatgtacctg aggaaatcag agagatggtg gctcctgtat taaaaagctt
961 ccaagccgag gtggtggccc ttagtaagag aagtcaggag gcggaggctg cttttctgag
1021 tgtttacaag caattaattg aagcaccaga ccccgtgcct gtgtttgagg cggcacgcag
1081 cctagacgac agactgcagc cccccagctt tgaccccagt gggcagcccc ggcgagacct
1141 ccacacttcg tggaagagga accccgagct cctcagcccc aaagagcaga gagaggggac
1201 gtcgcctgcc gggcccacgc tgaccgaggg aagccgcctc ccaggcattc ccgggaaagc
1261 cctcctgaca gaaaccttgc tgcagagaaa tgaggcggaa aaacaaaagg gccttcaaga
1321 agtacagatc actttggcgg ccagactggg ggaggcagag gagaaaatca aagtcctaca
1381 ttcagcgcta aaggctacgc aggcagagct gctagagctg cggcggaagt acgacgagga
1441 ggcagcatcc aaggcagatg aagtcggcct gatcatgacc aacctggaga aagctaatca
1501 gcgagctgag gctgcccagc gggaggtgga aagtctccgg gaacagctgg cctctgtcaa
1561 cagctccatc cgcctggctt gctgctctcc ccaggggccc agtggggata aggtgaactt
1621 cactctgtgc tcgggccctc ggctggaggc cgcgctggcc tccaaggaca gggagatcct
1681 gcggctgctg aaggacgtgc agcacctcca gagctcactg caggagctgg aggaggcatc
1741 cgccaaccag atcgccgacc tggagcggca gctcacggcc aagtccgagg ccatagaaaa
1801 gctggaagag aagctccagg cccagtctga ctatgaggaa attaaaacgg agctgagcat
1861 cctgaaagcc atgaagctgg cctccagcac ctgcagcctc ccccagggca tggccaagcc
1921 tgaagactca ctgcttattg caaaggaggc cttcttcccc acgcagaaat tccttctgga
1981 gaagcccagc ctcctggcca gccctgagga agacccatca gaggacgatt ccatcaagga
2041 ttcactgggc acggagcagt cctacccctc ccctcagcag ctcccacctc caccagggcc
2101 agaagacccc ctgtctccca gccctgggca gcccctgctg ggccccagct tggggcctga
2161 cggcactcgg actttctcgc tgtccccctt ccccagcctg gcatcagggg agagactgat
2221 gatgccccca gccgccttca agggagaggc gggcggcctg ctggtgttcc ccccagcctt
2281 ctatggcgcc aagcccccca cagcccctgc caccccggcc cctggccctg agccactggg
2341 cggtcctgag cccgcggatg gtggtggggg cggagcggcg gggcccgggg cagaggagga
2401 gcagctggac acggcagaga tcgccttcca ggtgaaggag cagctgctga aacacaacat
2461 cgggcagcgg gtgtttgggc attacgtgct ggggctgtcg cagggctcgg tcagcgagat
2521 cctagcccgg cccaagccct ggcgcaagct cacggtgaag ggcaaggagc ccttcatcaa
2581 gatgaagcag ttcctgtcgg atgagcagaa tgtactggcg ctcaggacca tccaagtgcg
2641 gcagcgaggc agcatcaccc cgagaatccg cacgcctgag acaggctcag acgacgccat
2701 caagagcatt ctagagcagg ccaagaagga gatcgagtcg cagaagggcg gcgagcccaa
2761 gacctcggtg gccccgctga gcatcgccaa cggcacgacc cccgccagca cctcggagga
2821 cgccatcaag agcatcctgg agcaggcacg ccgtgagatg caggcgcaac agcaggcgct
2881 gctggagatg gaggtggcgc ccaggggccg ctcggtgccc ccctcgcccc cggagcggcc
2941 atcactggcc actgcgagcc agaacggggc cccggccttg gtgaagcagg aggagggcag
3001 cgggggcccc gcgcaggcgc cgctcccggt cctgtccccc gccgccttcg tgcagagcat
3061 catccgcaag gtcaagtccg agatcggcga cgccggctac ttcgaccacc actgggcctc
3121 cgaccgcggc ctgctcagcc gcccctacgc ctccgtgtcg ccctcgctgt cctcctcctc
3181 ctcctctggc tactctggcc agcccaacgg ccgcgcctgg ccccgcgggg acgaggcccc
3241 tgtgcccccc gaggacgagg cggcggcagg taagtatcaa ggttacaaga caggtttaag
3301 gagaccaata agctattatt cgtataggat actttatacg aagttattaa gttatggaga
3361 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
3421 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
3481 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
3541 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
3601 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
3661 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
3721 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
3781 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
3841 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
3901 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
3961 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
4021 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
4081 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
4141 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
4201 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
4261 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
4321 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
4381 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
4441 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
4501 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
4561 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
4621 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
4681 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
4741 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
4801 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
4861 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
4921 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
4981 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
5041 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
5101 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
5161 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
5221 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
5281 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
5341 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5401 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5461 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5521 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5581 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
5641 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5701 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5761 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5821 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5881 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5941 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6001 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6061 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
6121 ctcacatgt
//
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