| 质粒名称: | pAAVLINK-EF1a-HA-5'-CUX1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001491 |
| 产品用途: | 5' AAVLINK plasmid for CUX1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CUX1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-CUX1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CUX1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CUX1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CUX1质粒载体简介
pAAVLINK-EF1a-HA-5'-CUX1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CUX1 5531 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5531)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5531
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..2693
/codon_start=1
/label=CUX1
/translation="LCVAGARLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTP
EDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKV
QRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEK
EQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETT
AKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEV
LTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLKQL
EEKLKGQADYEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALR
ISNSDLSGSARRKGKDQPESRRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQD
FFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSIS
SQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEMDTAEIARQVKEQLIKHNIGQRIFGHY
VLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQ
SLNRLFQEVPKRRNGSE"
misc_feature 1964..1969
/label=SDS
misc_feature 2021..2026
/label=SDS
intron 2694..2734
/label=Splicing Donor
misc_feature 2738..2771
/label=lox2272-JT1571
repeat_region 2816..2934
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2816..2923
/label=AAV2 ITR
rep_origin 3009..3464
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3746..3850
/gene="bla"
/label=AmpR promoter
CDS 3851..4711
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4882..5470
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctttgt gcgtagccgg agccaggttg aagagagaac tcgatgccac
781 cgcaacggta ttggcgaacc ggcaggatga aagtgagcag tccagaaagc ggcttatcga
841 acagagccgg gagttcaaga agaacactcc agaggatttg cgcaagcagg tagcgccgct
901 gctgaagagt ttccaaggag agattgatgc actgagtaaa agaagcaagg aagctgaagc
961 agctttcttg aatgtctaca aaagattgat tgacgtccca gatcccgtac cagctttgga
1021 tctcggacag caactccagc tcaaagtgca gcgcctgcac gatattgaaa cagagaacca
1081 gaaacttagg gaaactctgg aagaatacaa caaggaattt gctgaagtga aaaatcaaga
1141 ggttacgata aaagcactta aagagaaaat ccgagaatat gaacagacac tgaagaacca
1201 agccgaaacc atagctcttg agaaggaaca gaagttacag aatgactttg cagaaaagga
1261 gagaaagctg caggagacac agatgtccac cacctcaaag ctggaggaag ctgagcataa
1321 ggttcagagc ctacaaacag ccctggaaaa aactcgaaca gaattatttg acctgaaaac
1381 caaatacgat gaagaaacta ctgcaaaggc cgacgagatt gaaatgatca tgacggacct
1441 tgaaagggca aaccagaggg cagaggtggc tcagagagag gcggagacct taagggaaca
1501 gctctcatcg gccaatcact ccctccagct ggcctcacag atccagaagg caccagacgt
1561 ggagcaggcc atagaggtgc tgacccgctc cagcctagaa gttgagttgg ccgccaagga
1621 gcgggagatc gcacagctgg tggaggacgt gcagagactc caggccagcc tcaccaagct
1681 gcgggagaat tcggccagcc agatctcaca gcttgagcag cagctgagcg ccaaaaacag
1741 cacactcaaa caactggaag aaaaactcaa aggccaggct gactatgaag aggtgaagaa
1801 agagctgaac attctgaagt ccatggagtt tgcaccgtcc gagggcgctg ggacacagga
1861 tgcggccaag cccctggagg tgctgttgct ggagaagaac cgctcgctgc agtccgagaa
1921 cgccgcgctg cgcatctcca acagcgacct gagcgggtca gccaggagga aagggaaaga
1981 ccagcctgaa agtcggcgcc cgggatcttt gccggccccc cctccttctc agttgccccg
2041 caacccgggg gagcaggctt ccaatactaa tggtacacac cagttctcac cagcggggtt
2101 aagtcaagac tttttcagct catccctggc aagccccagc ctacccctgg cttctacagg
2161 aaaatttgca ctaaactctc ttctccagcg gcagctaatg cagtccttct actccaaggc
2221 tatgcaggaa gccggaagca caagcatgat tttttcaaca ggtccataca gcacaaactc
2281 catatcttcc caaagtccat tacaacaaag cccagatgtc aatggcatgg ccccatcccc
2341 cagccagtca gaaagtgctg ggagcgtctc cgagggcgag gagatggaca ctgcagaaat
2401 cgcccggcag gtcaaagagc agctgattaa gcacaatatc ggacaacgta ttttcggaca
2461 ttatgtgttg ggactgtctc aagggtccgt gagcgagatt ctggcccggc ccaagccatg
2521 gaataaactg actgttcgtg gcaaggagcc atttcacaag atgaaacagt tcctctccga
2581 tgagcagaac atcctggccc tccgtagcat ccaaggcaga caaagagaga atccaggcca
2641 gagcctgaac agactatttc aggaagtacc gaaacgaaga aatgggtctg aaggtaagta
2701 tcaaggttac aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta
2761 tacgaagtta ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
2821 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
2881 cgaccaaagg tcgcccggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
2941 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
3001 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
3061 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
3121 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
3181 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
3241 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
3301 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
3361 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
3421 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
3481 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
3541 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
3601 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
3661 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
3721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
3781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
3841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
3901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
3961 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
4021 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
4081 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
4141 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
4201 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
4261 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
4321 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
4381 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
4441 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
4501 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
4561 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
4621 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
4681 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
4741 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
4801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
4861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
4921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
4981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
5041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
5101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
5161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
5221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
5281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
5341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
5401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
5461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
5521 tgctcacatg t
//
