| 质粒名称: | pAAVLINK-EF1a-HA-5'-CECR2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001479 |
| 产品用途: | 5' AAVLINK plasmid for CECR2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CECR2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CECR2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CECR2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CECR2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CECR2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CECR2质粒载体简介
pAAVLINK-EF1a-HA-5'-CECR2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CECR2 6105 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6105)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6105
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3245
/codon_start=1
/label=CECR2
/translation="CPEEGGAAGLGELRSWWEVPAIAHFCSLFRTAFRLPDFEIEELEA
ALHRDDVEFISDLIACLLQGCYQRRDITPQTFHSYLEDIINYRWELEEGKPNPLREASF
QDLPLRTRVEILHRLCDYRLDADDVFDLLKGLDADSLRVEPLGEDNSGALYWYFYGTRM
YKEDPVQGKSNGELSLSRESEGQKNVSSIPGKTGKRRGRPPKRKKLQEEILLSEKQEEN
SLASEPQTRHGSQGPGQGTWWLLCQTEEEWRQVTESFRERTSLRERQLYKLLSEDFLPE
ICNMIAQKGKRPQRTKAELHPRWMSDHLSIKPVKQEETPVLTRIEKQKRKEEEEERQIL
LAVQKKEQEQMLKEERKRELEEKVKAVEDRAKRRKLREERAWLLAQGKELPPELSHLDP
NSPMREEKKTKDLFELDDDFTAMYKVLDVVKAHKDSWPFLEPVDESYAPNYYQIIKAPM
DISSMEKKLNGGLYCTKEEFVNDMKTMFRNCRKYNGESSEYTKMSDNLERCFHRAMMKH
FPGEDGDTDEEFWIREDEKREKRRSRAGRSGGSHVWTRSRDPEGSSRKQQPMENGGKSL
PPTRRAPSSGDDQSSSSTQPPREVGTSNGRGFSHPLHCGGTPSQAPFLNQMRPAVPGTF
GPLRGSDPATLYGSSGVPEPHHGEPVQQRQPFTMQPPVGINSLRGPRLGTPEEKQMCGG
LTHLSNMGPHPGSLQLGQISGPSQDGSMYAPAQFQPGFIPPRHGGAPARPPDFPESSEI
PPSHMYRSYKYLNRVHSAVWNGNHGATNQGPLGPDEKPHLGPGPSHQPRTLGHVMDSRV
MRPPVPPNQWTEQSGFLPHGVPSS"
misc_feature 744..749
/label=SDS
misc_feature 1365..1370
/label=SDS
misc_feature 1731..1736
/label=SDS
misc_feature 2160..2165
/label=SDS
intron 3246..3286
/label=Splicing Donor
misc_feature 3290..3323
/label=lox2272-JT1571
repeat_region 3368..3508
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3368..3497
/label=AAV2 ITR
rep_origin 3583..4038
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4320..4424
/gene="bla"
/label=AmpR promoter
CDS 4425..5285
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5456..6044
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatcttgcc cagaggaggg cggcgcggcc gggctgggcg agctccgctc
781 ctggtgggag gtcccggcca tcgcgcactt ctgctcgctc tttcgcaccg cgttccgcct
841 gcccgacttc gagatcgagg agttagaagc cgctcttcac agagatgacg tggagtttat
901 cagtgacctg attgcctgcc tgcttcaggg ctgctatcaa cgaagagata tcacgcctca
961 gacattccac agctacctag aggacatcat caactaccgc tgggagctcg aagaagggaa
1021 gcccaaccct ctgagggaag ccagtttcca ggacctgcct cttcgcacac gggtggagat
1081 cctgcaccga ctctgtgatt accggctgga tgcagacgat gtcttcgatc ttctaaaggg
1141 cctggatgca gacagtctcc gtgtggagcc attgggtgaa gacaattctg gggcactata
1201 ttggtatttc tatggaacac gaatgtacaa agaggacccg gtgcaaggaa aatccaatgg
1261 agaactctct ttgagcaggg aaagtgaagg acaaaaaaat gtctcaagta ttcctggaaa
1321 aacgggaaaa agaagaggaa gacccccaaa acggaagaaa ctgcaggagg agattctgtt
1381 gagtgaaaag caggaagaaa attccttggc atccgagcca cagacaagac atgggtccca
1441 agggccaggc caaggtactt ggtggctcct gtgccagaca gaagaggaat ggagacaggt
1501 caccgagagt tttcgcgaga ggacctccct tcgagaacgg cagctctaca agctcctcag
1561 tgaggacttc ctgcctgaga tctgcaacat gatcgcccag aagggaaaac gtccacagcg
1621 cacaaaggca gagttgcatc ctaggtggat gtctgaccac ctgtccatca aacccgtcaa
1681 gcaagaggag actcctgtgc tgaccagaat agaaaaacaa aagcgcaaag aggaggaaga
1741 agagcgtcag attcttctag cagtgcagaa gaaggagcag gagcagatgc taaaggaaga
1801 gaggaaacgc gagttggagg agaaggtcaa ggcagtggaa gatcgagcga agaggagaaa
1861 gctcagggaa gaaagggcat ggctgctggc tcaaggaaag gagctccctc cagaactttc
1921 ccatctggac cccaattccc ccatgagaga ggaaaaaaag actaaagacc tctttgagtt
1981 ggatgatgat ttcactgcta tgtataaagt tctagacgtg gtaaaggctc acaaggattc
2041 ctggcccttc ttggaacctg tggatgaatc ttatgcccct aactattatc agattattaa
2101 ggcccccatg gatatttcca gcatggagaa gaaactgaat ggaggtttat actgtaccaa
2161 ggaggaattt gtaaatgaca tgaagaccat gttcaggaat tgtcgaaagt ataatgggga
2221 aagtagtgag tataccaaga tgtctgataa tttagagagg tgtttccatc gggcaatgat
2281 gaaacatttt cctggagaag atggagacac agatgaagaa ttttggattc gagaggatga
2341 aaagcgggag aaaagacgga gtcgggctgg gcgaagtggt gggagccatg tttggacccg
2401 ctccagggac ccagaagggt ccagcaggaa acagcagccc atggagaatg gaggaaagtc
2461 gttgcccccc acacgccgag cgccctcttc tggggacgat cagagcagca gctccacaca
2521 gcccccgcgg gaggtgggca cttccaatgg ccgaggtttt tctcatcccc tgcattgtgg
2581 tgggacaccc agccaggcac cctttttaaa ccagatgagg ccagcagtac caggaacatt
2641 tggccctctg cgaggatcag atcctgccac cttgtatggc tcctctggag tcccggagcc
2701 acaccacggg gagcctgtgc agcagcgtca gcctttcacc atgcagcctc cagttggaat
2761 taacagcctc cgaggaccca ggctaggcac accagaggag aagcaaatgt gcggggggct
2821 gacacacctt tctaacatgg gcccacaccc tggatccttg cagcttgggc agataagtgg
2881 cccaagtcag gatggaagca tgtatgctcc agctcagttc cagccaggat tcattcctcc
2941 ccggcatggg ggggctccag cccggccacc agactttcct gaaagctcag aaattcctcc
3001 cagccatatg tatcgatcgt acaagtacct gaatcgagta cactctgccg tctggaatgg
3061 gaaccatggt gctacgaacc aaggaccctt gggcccagat gagaagcccc acctggggcc
3121 aggaccctct caccagcctc gcactctcgg tcacgtgatg gattcccgag tcatgagacc
3181 acctgtcccc cccaaccagt ggactgaaca atcaggcttc ctacctcatg gagttccttc
3241 ctcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta
3301 taggatactt tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag
3361 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
3421 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
3481 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
3541 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
3601 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
3661 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
3721 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
3781 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
3841 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
3901 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
3961 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
4021 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
4081 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
4141 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
4201 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
4261 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
4321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
4381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
4441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
4501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
4561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
4621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
4681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
4741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
4801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
4861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
4921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
4981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
5041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
5101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
5161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
5221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
5281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
5341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
5401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
5461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
5521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
5581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
5641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
5701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
5821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
5941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
6001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
6061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
