| 质粒名称: | pAAVLINK-EF1a-5'-ROBO2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001455 |
| 产品用途: | 5' AAVLINK plasmid for ROBO2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ROBO2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-5'-ROBO2载体质粒基本信息
pAAVLINK-EF1a-5'-ROBO2质粒图谱载体图谱和pAAVLINK-EF1a-5'-ROBO2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-ROBO2质粒载体简介
pAAVLINK-EF1a-5'-ROBO2质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-ROBO2 5991 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5991)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5991
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 701..3131
/codon_start=1
/label=ROBO2
/translation="MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKG
EPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPD
EGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEP
TIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVF
ERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIK
KTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCE
TKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALT
VAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKE
GFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATIS
KNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVA
NHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQ
KELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAK
VPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVG
SYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFP"
misc_feature 777..782
/label=SDS
misc_feature 1385..1390
/label=SDS
misc_feature 2070..2075
/label=SDS
misc_feature 2996..3001
/label=SDS
misc_feature 2999..3004
/label=SDS
intron 3132..3172
/label=Splicing Donor
misc_feature 3176..3209
/label=lox2272-JT1571
repeat_region 3254..3394
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3254..3383
/label=AAV2 ITR
rep_origin 3469..3924
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4206..4310
/gene="bla"
/label=AmpR promoter
CDS 4311..5171
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5342..5930
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgagtctgc tgatgtttac
721 acaactactg ctctgtggat ttttatatgt tcgggttgat ggatcgcgtc ttcgccagga
781 ggactttccc ccgcggattg tggagcatcc ttccgatgtc atcgtctcta agggcgagcc
841 cacgactctg aactgcaagg cggagggccg gccaacgccc accattgagt ggtacaaaga
901 tggggagcga gtggagactg acaaggacga tccccggtcc cacaggatgc ttctgcccag
961 cggatcctta ttcttcttgc gcatcgtgca cgggcgcagg agtaaacctg atgaaggaag
1021 ctacgtttgt gttgcgagga actatcttgg tgaagcagtg agtcgaaatg cgtctctgga
1081 agtggcattg ttacgagatg acttccgaca aaaccccaca gatgttgtag tggcagctgg
1141 agagcctgca atcctggagt gccagcctcc ccggggacac ccagaaccca ccatctactg
1201 gaaaaaagac aaagttcgaa ttgatgacaa ggaagaaaga ataagtatcc gtggtggaaa
1261 actgatgatc tccaatacca ggaaaagtga tgcagggatg tatacttgtg ttggtaccaa
1321 tatggtggga gaaagggaca gtgacccagc agagctgact gtctttgaac gacccacatt
1381 tctcaggagg ccaattaacc aggtggtact ggaggaagaa gctgtagaat ttcgttgtca
1441 agtccaagga gatcctcaac caactgtgag gtggaaaaag gatgatgcag acttgccaag
1501 aggaaggtat gacatcaaag acgattacac actaagaatt aaaaagacca tgagtacaga
1561 tgaaggcacc tatatgtgta ttgctgagaa tcgggttgga aaaatggaag cctctgctac
1621 actcaccgtc cgagctcgcc ctgttgctcc cccacagttt gtggttcggc caagagatca
1681 gattgttgct caaggtcgaa cagtgacatt tccctgtgaa actaaaggaa acccacagcc
1741 agctgttttt tggcagaaag aaggcagcca gaacctactt ttcccaaacc aaccccagca
1801 gcccaacagt agatgctcag tgtcaccaac tggagacctc acaatcacca acattcaacg
1861 ttccgacgcg ggttactaca tctgccaggc tttaactgtg gcaggaagca ttttagcaaa
1921 agctcaactg gaggttactg atgttttgac agatagacct ccacctataa ttctacaagg
1981 cccagccaac caaacgctgg cagtggatgg tacagcgtta ctgaaatgta aagccactgg
2041 tgatcctctt cctgtaatta gctggttaaa ggagggattt acttttccgg gtagagatcc
2101 aagagcaaca attcaagagc aaggcacact gcagattaag aatttacgga tttctgatac
2161 tggcacttat acttgtgtgg ctacaagttc aagtggagag acttcctgga gtgcagtgct
2221 ggatgtgaca gagtctggag caacaatcag taaaaactat gatttaagtg acctgccagg
2281 gccaccatcc aaaccgcagg tcactgatgt tactaagaac agtgtcacct tgtcctggca
2341 gccaggtacc cctggaaccc ttccagcaag tgcatatatc attgaggctt tcagccaatc
2401 agtgagcaac agctggcaga ccgtggcaaa ccatgtaaag accaccctct atactgtaag
2461 aggactgcgg cccaatacaa tctacttatt catggtcaga gcgatcaacc cccaaggtct
2521 cagtgaccca agtcccatgt cagatcctgt gcgcacacaa gatatcagcc caccagcaca
2581 aggagtggac cacaggcaag tgcagaaaga gctaggagat gtccttgtcc gtcttcataa
2641 tccagttgtg ctgactccca ccacggttca ggtcacatgg acggttgatc gccaacccca
2701 gtttatccaa ggctaccgag tgatgtatcg tcagacttca ggtctgcagg cgacatcttc
2761 gtggcagaat ttagatgcca aagtcccgac tgaacgaagt gctgtcttag tcaacctgaa
2821 aaagggggtg acttatgaaa ttaaagtacg gccatatttt aatgagttcc aaggaatgga
2881 tagtgaatct aaaacggttc gtactactga agaagcccca agtgccccac cacagtctgt
2941 cactgtactg acagttggaa gctacaatag cacaagtatt agtgtttcct gggatcctcc
3001 tcctccagat caccagaatg gaattatcca agaatacaag atctggtgtc taggaaatga
3061 aacgcgattc catatcaaca aaactgtgga tgcagccatt cggtccgtaa taattggtgg
3121 attattccca ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta
3181 ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg
3241 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
3301 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
3361 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
3421 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
3481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
3541 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
3601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
3661 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
3721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
3781 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
3841 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
3901 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
3961 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
4021 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
4081 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
4141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
4201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
4261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
4321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
4381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
4441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
4501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
4561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
4621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
4681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
4741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
4801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
4861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
4921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
4981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
5041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
5101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
5161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
5221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
5281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
5341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
5401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
5461 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
5521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
5581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
5641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
5701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
5761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
5821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
5881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
5941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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pAAVLINK-EF1a-5'-ROBO2质粒载体应用举例
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