| 质粒名称: | pAAVLINK-EF1a-5'-PCDH11X |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001473 |
| 产品用途: | 5' AAVLINK plasmid for PCDH11X expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PCDH11X |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-5'-PCDH11X载体质粒基本信息
pAAVLINK-EF1a-5'-PCDH11X质粒图谱载体图谱和pAAVLINK-EF1a-5'-PCDH11X载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-PCDH11X质粒载体简介
pAAVLINK-EF1a-5'-PCDH11X质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-PCDH11X 5663 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5663)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5663
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..2803
/codon_start=1
/label=PCDH11X
/translation="MDLLSGTYIFAVLLACVVFHSGAQEKNYTIREEMPENVLIGDLLK
DLNLSLIPNKSLTTAMQFKLVYKTGDVPLIRIEEDTGEIFTTGARIDREKLCAGIPRDE
HCFYEVEVAILPDEIFRLVKIRFLIEDINDNAPLFPATVINISIPENSAINSKYTLPAA
VDPDVGINGVQNYELIKSQNIFGLDVIETPEGDKMPQLIVQKELDREEKDTYVMKVKVE
DGGFPQRSSTAILQVSVTDTNDNHPVFKETEIEVSIPENAPVGTSVTQLHATDADIGEN
AKIHFSFSNLVSNIARRLFHLNATTGLITIKEPLDREETPNHKLLVLASDGGLMPARAM
VLVNVTDVNDNVPSIDIRYIVNPVNDTVVLSENIPLNTKIALITVTDKDADHNGRVTCF
TDHEIPFRLRPVFSNQFLLETAAYLDYESTKEYAIKLLAADAGKPPLNQSAMLFIKVKD
ENDNAPVFTQSFVTVSIPENNSPGIQLTKVSAMDADSGPNAKINYLLGPDAPPEFSLDC
RTGMLTVVKKLDREKEDKYLFTILAKDNGVPPLTSNVTVFVSIIDQNDNSPVFTHNEYN
FYVPENLPRHGTVGLITVTDPDYGDNSAVTLSILDENDDFTIDSQTGVIRPNISFDREK
QESYTFYVKAEDGGRVSRSSSAKVTINVVDVNDNKPVFIVPPSNCSYELVLPSTNPGTV
VFQ"
misc_feature 1960..1965
/label=SDS
misc_feature 2042..2047
/label=SDS
misc_feature 2738..2743
/label=SDS
intron 2804..2844
/label=Splicing Donor
misc_feature 2848..2881
/label=lox2272-JT1571
repeat_region 2926..3066
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2926..3055
/label=AAV2 ITR
rep_origin 3141..3596
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3878..3982
/gene="bla"
/label=AmpR promoter
CDS 3983..4843
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5014..5602
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggactt
721 gttgtccggg acgtacattt tcgcggtcct gctagcatgc gtggtgttcc actctggcgc
781 ccaggagaaa aactacacca tccgagaaga aatgccagaa aacgtcctga taggcgactt
841 gttgaaagac cttaacttgt cgctgattcc aaacaagtcc ttgacaactg ctatgcagtt
901 caagctagtg tacaagaccg gagatgtgcc actgattcga attgaagagg atactggtga
961 gatcttcact actggcgctc gcattgatcg tgagaaatta tgtgctggta tcccaaggga
1021 tgagcattgc ttttatgaag tggaggttgc cattttgccg gatgaaatat ttagactggt
1081 taagatacgt tttctgatag aagatataaa tgataatgca ccattgttcc cagcaacagt
1141 tatcaacata tcaattccag agaactcggc tataaactct aaatatactc tcccagcggc
1201 tgttgatcct gacgtaggaa taaacggagt tcaaaactac gaactaatta agagtcaaaa
1261 catttttggc ctcgatgtca ttgaaacacc agaaggagac aagatgccac aactgattgt
1321 tcaaaaggag ttagataggg aagagaagga tacctacgtg atgaaagtaa aggttgaaga
1381 tggtggcttt cctcaaagat ccagtactgc tattttgcaa gtgagtgtta ctgatacaaa
1441 tgacaaccac ccagtcttta aggagacaga gattgaagtc agtataccag aaaatgctcc
1501 tgtaggcact tcagtgacac agctccatgc cacagatgct gacataggtg aaaatgccaa
1561 gatccacttc tctttcagca atctagtctc caacattgcc aggagattat ttcacctcaa
1621 tgccaccact ggacttatca caatcaaaga accactggat agggaagaaa caccaaacca
1681 caagttactg gttttggcaa gtgatggtgg attgatgcca gcaagagcaa tggtgctggt
1741 aaatgttaca gatgtcaatg ataatgtccc atccattgac ataagataca tcgtcaatcc
1801 tgtcaatgac acagttgttc tttcagaaaa tattccactc aacaccaaaa ttgctctcat
1861 aactgtgacg gataaggatg cggaccataa tggcagggtg acatgcttca cagatcatga
1921 aatccctttc agattaaggc cagtattcag taatcagttc ctcctggaga ctgcagcata
1981 tcttgactat gagtccacaa aagaatatgc cattaaatta ctggctgcag atgctggcaa
2041 acctcctttg aatcagtcag caatgctctt catcaaagtg aaagatgaaa atgacaatgc
2101 tccagttttc acccagtctt tcgtaactgt ttctattcct gagaataact ctcctggcat
2161 ccagttgacg aaagtaagtg caatggatgc agacagtggg cctaatgcta agatcaatta
2221 cctgctaggc cctgatgctc cacctgaatt cagcctggat tgtcgtacag gcatgctgac
2281 tgtagtgaag aaactagata gagaaaaaga ggataaatat ttattcacaa ttctggcaaa
2341 agataacggg gtaccaccct taaccagcaa tgtcacagtc tttgtaagca ttattgatca
2401 gaatgacaat agcccagttt tcactcacaa tgaatacaac ttctatgtcc cagaaaacct
2461 tccaaggcat ggtacagtag gactaatcac tgtaactgat cctgattatg gagacaattc
2521 tgcagttacg ctctccattt tagatgagaa tgatgacttc accattgatt cacaaactgg
2581 tgtcatccga ccaaatattt catttgatag agaaaaacaa gaatcttaca ctttctatgt
2641 aaaggctgag gatggtggta gagtatcacg ttcttcaagt gccaaagtaa ccataaatgt
2701 ggttgatgtc aatgacaaca aaccagtttt cattgtccct ccttccaact gttcttatga
2761 attggttcta ccgtccacta atccaggcac agtggtcttt caggtaagta tcaaggttac
2821 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
2881 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
2941 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
3001 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
3061 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
3121 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
3181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
3241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
3301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
3361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
3421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
3481 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
3541 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
3601 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
3661 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
3721 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
3781 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
3841 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3901 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3961 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
4021 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
4081 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
4141 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
4201 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
4261 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
4321 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
4381 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
4441 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
4501 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
4561 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
4621 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
4681 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
4741 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
4801 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
4861 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
4921 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
4981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
5041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
5101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
5161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
5221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
5281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
5341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
5401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
5461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
5521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
5581 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
5641 ttgctggcct tttgctcaca tgt
//
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