| 质粒名称: | pAAVLINK-EF1a-5'-NRXN1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001449 |
| 产品用途: | 5' AAVLINK plasmid for NRXN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-5'-NRXN1载体质粒基本信息
pAAVLINK-EF1a-5'-NRXN1质粒图谱载体图谱和pAAVLINK-EF1a-5'-NRXN1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-NRXN1质粒载体简介
pAAVLINK-EF1a-5'-NRXN1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-NRXN1 5647 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5647)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5647
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..2787
/codon_start=1
/label=NRXN1
/translation="MGTALLQRGGCFLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPK
WNACCESEMSFQLKTRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPATLL
ADTPVNDGAWHSVRIRRQFRNTTLFIDQVEAKWVEVKSKRRDMTVFSGLFVGGLPPELR
AAALKLTLASVREREPFKGWIRDVRVNSSQVLPVDSGEVKLDDEPPNSGGGSPCEAGEE
GEGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKGKEE
YIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAV
SLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTG
YTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQ
GDPKMKIHGVVAFKCENVATLDPITFETPESFISLPKWNAKKTGSISFDFRTTEPNGLI
LFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGTIKIKALLKKVNDGEWY
HVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAGLVFPTEVWTAL
LNYGYVGCIRDLFIDGQSKDIRQMAEVQSTAGVKPSCSKETAKPCLSNPCKNNGMC"
misc_feature 1374..1379
/label=SDS
misc_feature 2365..2370
/label=SDS
misc_feature 2368..2373
/label=SDS
intron 2788..2828
/label=Splicing Donor
misc_feature 2832..2865
/label=lox2272-JT1571
repeat_region 2910..3050
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2910..3039
/label=AAV2 ITR
rep_origin 3125..3580
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3862..3966
/gene="bla"
/label=AmpR promoter
CDS 3967..4827
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4998..5586
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggggac
721 ggcgctgctc cagcgcgggg gctgttttct tctgtgcctc tcgctgctgc tcctgggctg
781 ctgggcggag ctgggcagcg ggctggagtt tccgggcgcc gagggccaat ggacgcgctt
841 ccccaagtgg aacgcctgct gcgagagcga gatgagcttc cagctcaaga ctcgcagcgc
901 ccgcggcctc gtgctctact tcgacgacga gggcttctgc gacttcctgg agctgattct
961 gacgcgcggc ggccgcctgc agctcagctt ctccatcttc tgcgctgagc ctgcgacgct
1021 cctggccgac acgccggtta acgacggcgc ctggcacagc gtgcgcatcc gccgccagtt
1081 ccgcaacacc acgctcttca tcgaccaggt ggaggccaag tgggtggagg tcaagtccaa
1141 gcgcagggac atgacggtgt tcagcggcct tttcgtcggg gggctgcccc cggaactgcg
1201 cgccgcggcg ctcaagctca ccctggcctc ggtgagggag cgggagccct tcaaggggtg
1261 gattcgtgac gtgagggtca actcctcgca ggtcctgccc gtggacagcg gcgaggtgaa
1321 gctggacgat gagccgccca acagcggcgg gggaagcccg tgcgaggcgg gcgaggaggg
1381 cgagggcggg gtgtgcctca acggaggtgt gtgctccgtg gtggacgacc aggccgtgtg
1441 cgactgctcg cgaaccggct tccgcggcaa ggactgcagc caagaagaca acaatgtgga
1501 aggtctggcg cacctgatga tgggcgacca aggtaaaagt aaaggaaaag aagaatatat
1561 tgccacgttc aaaggatctg aatacttctg ctacgacttg tctcaaaacc ccattcaaag
1621 cagcagtgat gaaataactc tgtcatttaa aacccttcag aggaatggac tgatgcttca
1681 cactgggaaa tcggctgatt atgtcaatct tgccctgaaa aatggagctg tctctctggt
1741 cattaatttg ggatcagggg cctttgaagc actagtggag cctgtgaatg gaaagtttaa
1801 tgataatgcc tggcatgatg tgaaagtcac caggaatctg cgtcagcact caggcattgg
1861 acacgctatg gtgacaatat cagtggatgg gattcttacc acaacgggct acacgcaaga
1921 agattatacc atgctggggt ctgatgactt tttctatgtt ggaggcagtc ccagcacagc
1981 cgaccttcca gggtcaccag tcagtaacaa ctttatgggc tgtctcaaag aggttgtata
2041 taaaaataat gatgtgaggc tggaattatc tcgacttgcc aagcaaggag atcctaagat
2101 gaagatccat ggagtggtgg catttaaatg tgagaatgtt gcaactttag acccaatcac
2161 ctttgaaacc ccagagtctt tcatctcttt gcctaaatgg aatgcaaaga aaactggctc
2221 catatcattt gatttccgta caacagagcc aaatggcctc atcttattta gccatggcaa
2281 gccaagacat cagaaagatg ccaagcaccc acagatgata aaggtggact tctttgctat
2341 tgagatgcta gatggccacc tctacctcct cctggacatg gggtcaggta ctataaaaat
2401 aaaagccctg ttgaagaaag tgaatgatgg agaatggtat catgtggact tccagagaga
2461 cggacggtca ggtaccattt ctgtcaacac gttgcgtact ccctacactg ctcctggtga
2521 gagtgagatt ctggacctgg atgatgagtt gtacctgggg gggctgccag aaaataaagc
2581 tggccttgtc ttccccaccg aggtgtggac tgctctgctc aactatggct acgtgggctg
2641 catcagggat ttgttcatcg atggccaaag caaagatatc cggcaaatgg ctgaagttca
2701 aagtactgct ggagtgaagc cttcctgctc aaaggaaaca gcaaaaccgt gccttagcaa
2761 cccttgcaaa aacaatggca tgtgcaggta agtatcaagg ttacaagaca ggtttaagga
2821 gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc
2881 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
2941 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
3001 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
3061 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
3121 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
3181 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
3241 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
3301 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
3361 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
3421 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
3481 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
3541 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
3601 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
3661 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
3721 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
3781 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
3841 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
3901 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
3961 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
4021 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
4081 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
4141 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
4201 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
4261 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
4321 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
4381 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
4441 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
4501 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
4561 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
4621 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
4681 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
4741 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
4801 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
4861 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
4921 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
4981 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
5041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
5101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
5161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
5221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
5281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
5341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
5401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
5461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
5521 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
5581 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
5641 cacatgt
//
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