| 质粒名称: | pAAVLINK-EF1a-5'-LAMB1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001554 |
| 产品用途: | 5' AAVLINK plasmid for LAMB1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | LAMB1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-LAMB1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-LAMB1载体质粒基本信息
pAAVLINK-EF1a-5'-LAMB1质粒图谱载体图谱和pAAVLINK-EF1a-5'-LAMB1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-LAMB1质粒载体简介
pAAVLINK-EF1a-5'-LAMB1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-LAMB1 6970 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6970)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6970
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
regulatory 695..704
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 701..4110
/codon_start=1
/label=LAMB1
/translation="MGLLQLLAFSFLALCRARVRAQEPEFSYGCAEGSCYPATGDLLIG
RAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICNSQDPYHETLNPDSHLIENVVTTFA
PNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWGVY
RYFAYDCEASFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSP
RIQNLLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECA
PVDGFNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEH
SISCHFDMAVYLATGNVSGGVCDDCQHNTMGRNCEQCKPFYYQHPERDIRDPNFCERCT
CDPAGSQNEGICDSYTDFSTGLIAGQCRCKLNVEGEHCDVCKEGFYDLSSEDPFGCKSC
ACNPLGTIPGGNPCDSETGHCYCKRLVTGQHCDQCLPEHWGLSNDLDGCRPCDCDLGGA
LNNSCFAESGQCSCRPHMIGRQCNEVEPGYYFATLDHYLYEAEEANLGPGVSIVERQYI
QDRIPSWTGAGFVRVPEGAYLEFFIDNIPYSMEYDILIRYEPQLPDHWEKAVITVQRPG
RIPTSSRCGNTIPDDDNQVVSLSPGSRYVVLPRPVCFEKGTNYTVRLELPQYTSSDSDV
ESPYTLIDSLVLMPYCKSLDIFTVGGSGDGVVTNSAWETFQRYRCLENSRSVVKTPMTD
VCRNIIFSISALLHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFG
FGPSGCKPCECHLQGSVNAFCNPVTGQCHCFQGVYARQCDRCLPGHWGFPSCQPCQCNG
HADDCDPVTGECLNCQDYTMGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFA
RSCYQDPVTLQLACVCDPGYIGSRCDDCASGYFGNPSEVGGSCQPCQCHNNIDTTDPEA
CDKETGRCLKCLYHTEGEHCQFCRFGYYGDALQQDCRKCVCNYLGTVQEHCNGSDCQCD
KATGQCLCLPNVIGQNCDRCAPNTWQLASGTGCDPCNCNAAHSFGPSCNEFTGQCQCMP
GFGGRTCSECQELFWGDPDVECRACDCDP"
misc_feature 912..917
/label=SDS
misc_feature 2442..2447
/label=SDS
misc_feature 2763..2768
/label=SDS
intron 4111..4151
/label=Splicing Donor
misc_feature 4155..4188
/label=lox2272-JT1571
repeat_region 4233..4373
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4233..4362
/label=AAV2 ITR
rep_origin 4448..4903
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5185..5289
/gene="bla"
/label=AmpR promoter
CDS 5290..6150
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6321..6909
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atggggcttc tccagttgct
721 agctttcagt ttcttagccc tgtgcagagc ccgagtgcgc gctcaggaac ccgagttcag
781 ctacggctgc gcagaaggca gctgctatcc cgccacgggc gaccttctca tcggccgagc
841 acagaagctt tcggtgacct cgacgtgcgg gctgcacaag cccgaaccct actgtatcgt
901 cagccacttg caggaggaca aaaaatgctt catatgcaat tcccaagatc cttatcatga
961 gaccctgaat cctgacagcc atctcattga aaatgtggtc actacatttg ctccaaaccg
1021 ccttaagatt tggtggcaat ctgaaaatgg tgtggaaaat gtaactatcc aactggattt
1081 ggaagcagaa ttccatttta ctcatctcat aatgactttc aagacattcc gtccagctgc
1141 tatgctgata gaacgatcgt ccgactttgg gaaaacctgg ggtgtgtata gatacttcgc
1201 ctatgactgt gaggcctcgt ttccaggcat ttcaactggc cccatgaaaa aagtcgatga
1261 cataatttgt gattctcgat attctgacat tgaaccctca actgaaggag aggtgatatt
1321 tcgtgcttta gatcctgctt tcaaaataga agatccttat agcccaagga tacagaattt
1381 attaaaaatt accaacttga gaatcaagtt tgtgaaactg catactttgg gagataacct
1441 tctggattcc aggatggaaa tcagagaaaa gtattattat gcagtttatg atatggtggt
1501 tcgaggaaat tgcttctgct atggtcatgc cagcgaatgt gcccctgtgg atggattcaa
1561 tgaagaagtg gaaggaatgg ttcacggaca ctgcatgtgc aggcataaca ccaagggctt
1621 aaactgtgaa ctctgcatgg atttctacca tgatttacct tggagacctg ctgaaggccg
1681 aaacagcaac gcctgtaaaa aatgtaactg caatgaacat tccatctctt gtcactttga
1741 catggctgtt tacctggcca cggggaacgt cagcggaggc gtgtgtgatg actgtcagca
1801 caacaccatg gggcgcaact gtgagcagtg caagccgttt tactaccagc acccagagag
1861 ggacatccga gatcctaatt tctgtgaacg atgtacgtgt gacccagctg gctctcaaaa
1921 tgagggaatt tgtgacagct atactgattt ttctactggt ctcattgctg gccagtgtcg
1981 gtgtaaatta aatgtggaag gagaacattg tgatgtttgc aaagaaggct tctatgattt
2041 aagcagtgaa gatccatttg gttgtaaatc ttgtgcttgc aatcctctgg gaacaattcc
2101 tggagggaat ccttgtgatt ccgagacagg tcactgctac tgcaagcgtc tggtgacagg
2161 acagcattgt gaccagtgcc tgccagagca ctggggctta agcaatgatt tggatggatg
2221 tcgaccatgt gactgtgacc ttgggggagc cttaaacaac agttgctttg cggagtcagg
2281 ccagtgctca tgccggcctc acatgattgg acgtcagtgc aacgaagtgg aacctggtta
2341 ctactttgcc accctggatc actacctcta tgaagcggag gaagccaact tggggcctgg
2401 ggttagcata gtggagcggc aatatatcca ggaccggatt ccctcctgga ctggagccgg
2461 cttcgtccga gtgcctgaag gggcttattt ggagtttttc attgacaaca taccatattc
2521 catggagtac gacatcctaa ttcgctacga gccacagcta cccgaccact gggaaaaagc
2581 tgtcatcaca gtgcagcgac ctggaaggat tccaaccagc agccgatgtg gtaataccat
2641 ccccgatgat gacaaccagg tggtgtcatt atcaccaggc tcaagatatg tcgtccttcc
2701 tcggccggtg tgctttgaga agggaacaaa ctacacggtg aggttggagc tgcctcagta
2761 cacctcctct gatagcgacg tggagagccc ctacacgctg atcgattctc ttgttctcat
2821 gccatactgt aaatcactgg acatcttcac cgtgggaggt tcaggagatg gggtggtcac
2881 caacagtgcc tgggaaacct ttcagagata ccgatgtcta gagaacagca gaagcgttgt
2941 gaaaacaccg atgacagatg tttgcagaaa catcatcttt agcatttctg ccctgttaca
3001 ccagacaggc ctggcttgtg aatgcgaccc tcagggttcg ttaagttccg tgtgtgatcc
3061 caacggaggc cagtgccagt gccggcccaa cgtggttgga agaacctgca acagatgtgc
3121 acctggaact tttggctttg gccccagtgg atgcaaacct tgtgagtgcc atctgcaagg
3181 atctgtcaat gccttctgca atcccgtcac tggccagtgc cactgtttcc agggagtgta
3241 tgctcggcag tgtgatcggt gcttacctgg gcactggggc tttccaagtt gccagccctg
3301 ccagtgcaat ggccacgccg atgactgcga cccagtgact ggggagtgct tgaactgcca
3361 ggactacacc atgggtcata actgtgaaag gtgcttggct ggttactatg gcgaccccat
3421 cattgggtca ggagatcact gccgcccttg cccttgccca gatggtcccg acagtggacg
3481 ccagtttgcc aggagctgct accaagatcc tgttacttta cagcttgcct gtgtttgtga
3541 tcctggatac attggttcca gatgtgacga ctgtgcctca ggatactttg gcaatccatc
3601 agaagttggg gggtcgtgtc agccttgcca gtgtcacaac aacattgaca cgacagaccc
3661 agaagcctgt gacaaggaga ctgggaggtg tctcaagtgc ctgtaccaca cggaagggga
3721 acactgtcag ttctgccggt ttggatacta tggtgatgcc ctccagcagg actgtcgaaa
3781 gtgtgtctgt aattacctgg gcaccgtgca agagcactgt aacggctctg actgccagtg
3841 cgacaaagcc actggtcagt gcttgtgtct tcctaatgtg atcgggcaga actgtgaccg
3901 ctgtgcgccc aatacctggc agctggccag tggcactggc tgtgacccat gcaactgcaa
3961 tgctgctcat tccttcgggc catcttgcaa tgagttcacg gggcagtgcc agtgcatgcc
4021 tgggtttgga ggccgcacct gcagcgagtg ccaggaactc ttctggggag accccgacgt
4081 ggagtgccga gcctgtgact gtgaccccag gtaagtatca aggttacaag acaggtttaa
4141 ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta agttatggag
4201 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
4261 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
4321 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
4381 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
4441 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4501 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
4561 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4621 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
4681 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4741 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
4801 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
4861 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
4921 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
4981 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
5041 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
5101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
5161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
5221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
5281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
5341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
5401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
5461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
5521 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
5581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
5641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
5701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
5761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
5821 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
5881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
5941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
6001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
6061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
6121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
6181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
6241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
6301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
6361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
6421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
6481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
6541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
6601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
6661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
6721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
6781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
6841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
6901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
6961 gctcacatgt
//
