| 质粒名称: | pAAVLINK-EF1a-5'-GRIN2B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001353 |
| 产品用途: | 5' AAVLINK plasmid for GRIN2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GRIN2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-5'-GRIN2B载体质粒基本信息
pAAVLINK-EF1a-5'-GRIN2B质粒图谱载体图谱和pAAVLINK-EF1a-5'-GRIN2B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-GRIN2B质粒载体简介
pAAVLINK-EF1a-5'-GRIN2B质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-GRIN2B 6619 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6619)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6619
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3759
/codon_start=1
/gene="GRIN2B"
/gene_synonym="DEE27; EIEE27; GluN2B; hNR3; MRD6;
NMDAR2B;NR2B; NR3"
/product="glutamate receptor ionotropic, NMDA 2B isoform a
precursor"
/label=GRIN2B
/note="isoform a precursor is encoded by transcriptvariant
1; glutamate [NMDA] receptor subunit epsilon-2; glutamate
receptor subunit epsilon-2; N-methyl-D-aspartate receptor
subunit 3; N-methyl D-aspartate receptor subtype 2B;
GluN2B(alt_5'UTR); glutamate receptor ionotropic, NMDA 2B;
glutamate receptor, ionotropic, N-methyl D-aspartate 2B"
/db_xref="GI:167003331"
/db_xref="CCDS:CCDS8662.1"
/db_xref="GeneID:2904"
/db_xref="HGNC:HGNC:4586"
/db_xref="MIM:138252"
/protein_id="NP_000825.2"
/translation="MKPRAECCSPKFWLVLAVLAVSGSRARSQKSPPSIGIAVILVGTS
DEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTD
QEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEE
YDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKK
LQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPAEFPTGLISVS
YDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLI
NVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETE
EQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIVTENKTDEEPGYIKKCCK
GFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINE
ERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFV
FEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVW
AFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERN
IRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGS
GKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQ
LDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSC
IHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESS
VYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHH
RPHSIGSASSIDGLYDCDNPPFTTQS"
misc_feature 1311..1316
/label=SDS
misc_feature 1318..1323
/label=SDS
misc_feature 1914..1919
/label=SDS
misc_feature 1947..1952
/label=SDS
intron 3760..3800
/label=Splicing Donor
misc_feature 3804..3837
/label=lox2272-JT1571
repeat_region 3882..4022
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3882..4011
/label=AAV2 ITR
rep_origin 4097..4552
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4834..4938
/gene="bla"
/label=AmpR promoter
CDS 4939..5799
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5970..6558
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgaagcc
721 cagagcggag tgctgttctc ccaagttctg gttggtgttg gccgtcctgg ccgtgtcagg
781 cagcagagct cgttctcaga agagcccccc cagcattggc attgctgtca tcctcgtggg
841 cacttccgac gaggtggcca tcaaggatgc ccacgagaaa gatgatttcc accatctctc
901 cgtggtaccc cgggtggaac tggtagccat gaatgagacc gacccaaaga gcatcatcac
961 ccgcatctgt gatctcatgt ctgaccggaa gatccagggg gtggtgtttg ctgatgacac
1021 agaccaggaa gccatcgccc agatcctcga tttcatttca gcacagactc tcacccccat
1081 cctgggcatc cacgggggct cctctatgat aatggcagat aaggatgaat cctccatgtt
1141 cttccagttt ggcccatcaa ttgaacagca agcttccgta atgctcaaca tcatggaaga
1201 atatgactgg tacatctttt ctatcgtcac cacctatttc cctggctacc aggactttgt
1261 aaacaagatc cgcagcacca ttgagaatag ctttgtgggc tgggagctag aggaggtcct
1321 cctactggac atgtccctgg acgatggaga ttctaagatc cagaatcagc tcaagaaact
1381 tcaaagcccc atcattcttc tttactgtac caaggaagaa gccacctaca tctttgaagt
1441 ggccaactca gtagggctga ctggctatgg ctacacgtgg atcgtgccca gtctggtggc
1501 aggggataca gacacagtgc ctgcggagtt ccccactggg ctcatctctg tatcatatga
1561 tgaatgggac tatggcctcc ccgccagagt gagagatgga attgccataa tcaccactgc
1621 tgcttctgac atgctgtctg agcacagctt catccctgag cccaaaagca gttgttacaa
1681 cacccacgag aagagaatct accagtccaa tatgctaaat aggtatctga tcaatgtcac
1741 ttttgagggg aggaatttgt ccttcagtga agatggctac cagatgcacc cgaaactggt
1801 gataattctt ctgaacaagg agaggaagtg ggaaagggtg gggaagtgga aagacaagtc
1861 cctgcagatg aagtactatg tgtggccccg aatgtgtcca gagactgaag agcaggagga
1921 tgaccatctg agcattgtga ccctggagga ggcaccattt gtcattgtgg aaagtgtgga
1981 ccctctgagt ggaacctgca tgaggaacac agtcccctgc caaaaacgca tagtcactga
2041 gaataaaaca gacgaggagc cgggttacat caaaaaatgc tgcaaggggt tctgtattga
2101 catccttaag aaaatttcta aatctgtgaa gttcacctat gacctttacc tggttaccaa
2161 tggcaagcat gggaagaaaa tcaatggaac ctggaatggt atgattggag aggtggtcat
2221 gaagagggcc tacatggcag tgggctcact caccatcaat gaggaacgat cggaggtggt
2281 cgacttctct gtgcccttca tagagacagg catcagtgtc atggtgtcac gcagcaatgg
2341 gactgtctca ccttctgcct tcttagagcc attcagcgct gacgtatggg tgatgatgtt
2401 tgtgatgctg ctcatcgtct cagccgtggc tgtctttgtc tttgagtact tcagccctgt
2461 gggttataac aggtgcctcg ctgatggcag agagcctggt ggaccctctt tcaccatcgg
2521 caaagctatt tggttgctct ggggtctggt gtttaacaac tccgtacctg tgcagaaccc
2581 aaaggggacc acctccaaga tcatggtgtc agtgtgggcc ttctttgctg tcatcttcct
2641 ggccagctac actgccaact tagctgcctt catgatccaa gaggaatatg tggaccaggt
2701 ttctggcctg agcgacaaaa agttccagag acctaatgac ttctcacccc ctttccgctt
2761 tgggaccgtg cccaacggca gcacagagag aaatattcgc aataactatg cagaaatgca
2821 tgcctacatg ggaaagttca accagagggg tgtagatgat gcattgctct ccctgaaaac
2881 agggaaactg gatgccttca tctatgatgc agcagtgctg aactatatgg caggcagaga
2941 tgaaggctgc aagctggtga ccattggcag tgggaaggtc tttgcttcca ctggctatgg
3001 cattgccatc caaaaagatt ctgggtggaa gcgccaggtg gaccttgcta tcctgcagct
3061 ctttggagat ggggagatgg aagaactgga agctctctgg ctcactggca tttgtcacaa
3121 tgagaagaat gaggtcatga gcagccagct ggacattgac aacatggcag gggtcttcta
3181 catgttgggg gcggccatgg ctctcagcct catcaccttc atctgcgaac accttttcta
3241 ttggcagttc cgacattgct ttatgggtgt ctgttctggc aagcctggca tggtcttctc
3301 catcagcaga ggtatctaca gctgcatcca tggggtggcg atcgaggagc gccagtctgt
3361 aatgaactcc cccaccgcaa ccatgaacaa cacacactcc aacatcctgc gcctgctgcg
3421 cacggccaag aacatggcta acctgtctgg tgtgaatggc tcaccgcaga gcgccctgga
3481 cttcatccga cgggagtcat ccgtctatga catctcagag caccgccgca gcttcacgca
3541 ttctgactgc aaatcctaca acaacccgcc ctgtgaggag aacctcttca gtgactacat
3601 cagtgaggta gagagaacgt tcgggaacct gcagctgaag gacagcaacg tgtaccaaga
3661 tcactaccac catcaccacc ggccccatag tattggcagt gccagctcca tcgatgggct
3721 ctacgactgt gacaacccac ccttcaccac ccagtccagg taagtatcaa ggttacaaga
3781 caggtttaag gagaccaata agctattatt cgtataggat actttatacg aagttattaa
3841 gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
3901 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
3961 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
4021 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
4081 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
4141 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
4201 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
4261 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
4321 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
4381 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
4441 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
4501 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
4561 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
4621 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
4681 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
4741 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
4801 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
4861 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
4921 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
4981 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
5041 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
5101 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
5161 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
5221 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
5281 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
5341 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
5401 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
5461 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
5521 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
5581 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
5641 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
5701 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
5761 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
5821 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
5881 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
5941 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
6001 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
6061 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
6121 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
6181 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
6241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
6361 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
6421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
6481 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
6541 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
6601 tggccttttg ctcacatgt
//
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