| 质粒名称: | pAAVLINK-EF1a-5'-GRIN2A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001446 |
| 产品用途: | 5' AAVLINK plasmid for GRIN2A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GRIN2A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-5'-GRIN2A载体质粒基本信息
pAAVLINK-EF1a-5'-GRIN2A质粒图谱载体图谱和pAAVLINK-EF1a-5'-GRIN2A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-GRIN2A质粒载体简介
pAAVLINK-EF1a-5'-GRIN2A质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-GRIN2A 6144 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6144)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6144
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..3306
/codon_start=1
/label=GRIN2A
/translation="MGRVGYWTLLVLPALLVWRGPAPSAAAEKGPPALNIAVMLGHSHD
VTERELRTLWGPEQAAGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDT
DQEAVAQMLDFISSHTFVPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQ
DYDWHVFSLVTTIFPGYREFISFVKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKI
HSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSY
DDWDYSLEARVRDGIGILTTAASSMLEKFSYIPEAKASCYGQMERPEVPMHTLHPFMVN
VTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENHTLSLRHAVWPRYKSFSDC
EPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKG
FCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEE
RSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVF
EYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWA
FFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNI
RNNYPYMHQYMTKFNQKGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSG
YIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQL
DIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSIS"
misc_feature 1029..1034
/label=SDS
misc_feature 1947..1952
/label=SDS
regulatory 3188..3197
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
intron 3307..3347
/label=Splicing Donor
misc_feature 3351..3384
/label=lox2272-JT1571
repeat_region 3429..3547
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3429..3536
/label=AAV2 ITR
rep_origin 3622..4077
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4359..4463
/gene="bla"
/label=AmpR promoter
CDS 4464..5324
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5495..6083
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgggcag
721 agtgggctat tggaccctgc tggtgctgcc ggcccttctg gtctggcgcg gtccggcgcc
781 gagcgcggcg gcggagaagg gtccccccgc gctaaatatt gcggtgatgc tgggtcacag
841 ccacgacgtg acagagcgcg aacttcgaac actgtggggc cccgagcagg cggcggggct
901 gcccctggac gtgaacgtgg tagctctgct gatgaaccgc accgacccca agagcctcat
961 cacgcacgtg tgcgacctca tgtccggggc acgcatccac ggcctcgtgt ttggggacga
1021 cacggaccag gaggccgtag cccagatgct ggattttatc tcctcccaca ccttcgtccc
1081 catcttgggc attcatgggg gcgcatctat gatcatggct gacaaggatc cgacgtctac
1141 cttcttccag tttggagcgt ccatccagca gcaagccacg gtcatgctga agatcatgca
1201 ggattatgac tggcatgtct tctccctggt gaccactatc ttccctggct acagggaatt
1261 catcagcttc gtcaagacca cagtggacaa cagctttgtg ggctgggaca tgcagaatgt
1321 gatcacactg gacacttcct ttgaggatgc aaagacacaa gtccagctga agaagatcca
1381 ctcttctgtc atcttgctct actgttccaa agacgaggct gttctcattc tgagtgaggc
1441 ccgctccctt ggcctcaccg ggtatgattt cttctggatt gtccccagct tggtctctgg
1501 gaacacggag ctcatcccaa aagagtttcc atcgggactc atttctgtct cctacgatga
1561 ctgggactac agcctggagg cgagagtgag ggacggcatt ggcatcctaa ccaccgctgc
1621 atcttctatg ctggagaagt tctcctacat ccccgaggcc aaggccagct gctacgggca
1681 gatggagagg ccagaggtcc cgatgcacac cttgcaccca tttatggtca atgttacatg
1741 ggatggcaaa gacttatcct tcactgagga aggctaccag gtgcacccca ggctggtggt
1801 gattgtgctg aacaaagacc gggaatggga aaaggtgggc aagtgggaga accatacgct
1861 gagcctgagg cacgccgtgt ggcccaggta caagtccttc tccgactgtg agccggatga
1921 caaccatctc agcatcgtca ccctggagga ggccccattc gtcatcgtgg aagacataga
1981 ccccctgacc gagacgtgtg tgaggaacac cgtgccatgt cggaagttcg tcaaaatcaa
2041 caattcaacc aatgagggga tgaatgtgaa gaaatgctgc aaggggttct gcattgatat
2101 tctgaagaag ctttccagaa ctgtgaagtt tacttacgac ctctatctgg tgaccaatgg
2161 gaagcatggc aagaaagtta acaatgtgtg gaatggaatg atcggtgaag tggtctatca
2221 acgggcagtc atggcagttg gctcgctcac catcaatgag gaacgttctg aagtggtgga
2281 cttctctgtg ccctttgtgg aaacgggaat cagtgtcatg gtttcaagaa gtaatggcac
2341 cgtctcacct tctgcttttc tagaaccatt cagcgcctct gtctgggtga tgatgtttgt
2401 gatgctgctc attgtttctg ccatagctgt ttttgtcttt gaatacttca gccctgttgg
2461 atacaacaga aacttagcca aagggaaagc accccatggg ccttctttta caattggaaa
2521 agctatatgg cttctttggg gcctggtgtt caataactcc gtgcctgtcc agaatcctaa
2581 agggaccacc agcaagatca tggtatctgt atgggccttc ttcgctgtca tattcctggc
2641 tagctacaca gccaatctgg ctgccttcat gatccaagag gaatttgtgg accaagtgac
2701 cggcctcagt gacaaaaagt ttcagagacc tcatgactat tccccacctt ttcgatttgg
2761 gacagtgcct aatggaagca cggagagaaa cattcggaat aactatccct acatgcatca
2821 gtacatgacc aaatttaatc agaaaggagt agaggacgcc ttggtcagcc tgaaaacggg
2881 gaagctggac gctttcatct acgatgccgc agtcttgaat tacaaggctg ggagggatga
2941 aggctgcaag ctggtgacca tcgggagtgg gtacatcttt gccaccaccg gttatggaat
3001 tgcccttcag aaaggctctc cttggaagag gcagatcgac ctggccttgc ttcagtttgt
3061 gggtgatggt gagatggagg agctggagac cctgtggctc actgggatct gccacaacga
3121 gaagaacgag gtgatgagca gccagctgga cattgacaac atggcgggcg tattctacat
3181 gctggctgcc gccatggccc ttagcctcat caccttcatc tgggagcacc tcttctactg
3241 gaagctgcgc ttctgtttca cgggcgtgtg ctccgaccgg cctgggttgc tcttctccat
3301 cagcaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt
3361 ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga
3421 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
3481 cactgaggcc gggcgaccaa aggtcgcccg gcctcagtga gcgagcgagc gcgcagctgc
3541 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
3601 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
3661 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
3721 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
3781 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
3841 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
3901 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
3961 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
4021 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
4081 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
4141 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
4201 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
4261 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
4321 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
4381 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
4441 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
4501 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
4561 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
4621 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
4681 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
4741 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
4801 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
4861 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
4921 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
4981 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
5041 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
5101 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
5161 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
5221 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
5281 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
5341 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
5401 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
5461 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5521 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5581 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5641 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5701 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
5761 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
5821 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
5881 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
5941 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
6001 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
6061 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
6121 tttgctggcc ttttgctcac atgt
//
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pAAVLINK-EF1a-5'-GRIN2A质粒载体应用举例
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