| 质粒名称: | pAAVLINK-EF1a-5'-C4B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001551 |
| 产品用途: | 5' AAVLINK plasmid for C4B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
C4B
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-C4B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-C4B载体质粒基本信息
pAAVLINK-EF1a-5'-C4B质粒图谱载体图谱和pAAVLINK-EF1a-5'-C4B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-C4B质粒载体简介
pAAVLINK-EF1a-5'-C4B质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-C4B 6925 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6925)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6925
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 701..4087
/codon_start=1
/label=C4B
/translation="MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQD
VPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLR
GPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFAL
DQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDG
LESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAY
VRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYV
AAAIIEYPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPAS
GIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIA
RLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVF
MNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVD
GAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGC
GPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVNFQKAINEKLGQY
ASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAG
LQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLS
LSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSP
VEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKV
LQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTL
GSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDL
IQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWL
LSQQQADGSFQDLSPVIHRSMQ"
misc_feature 1266..1271
/label=SDS
misc_feature 1758..1763
/label=SDS
misc_feature 2101..2106
/label=SDS
misc_feature 2144..2149
/label=SDS
misc_feature 2316..2321
/label=SDS
misc_feature 2985..2990
/label=SDS
misc_feature 2988..2993
/label=SDS
protein_bind 3002..3013
/label=ZF binding site
/bound_moiety="CCR5TC zinc-finger domain"
/note="target sequence for the CCR5TC zinc-finger domain
from the CCR5-224 (+) zinc-finger nuclease (Perez et al.,
2008; Gross et al., 2013)"
misc_feature 3691..3696
/label=SDS
misc_feature 3979..3984
/label=SDS
intron 4088..4128
/label=Splicing Donor
misc_feature 4132..4165
/label=lox2272-JT1571
repeat_region 4210..4328
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4210..4317
/label=AAV2 ITR
rep_origin 4403..4858
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5140..5244
/gene="bla"
/label=AmpR promoter
CDS 5245..6105
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6276..6864
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgaggctgc tctgggggct
721 gatctgggca tccagcttct tcaccttatc tctgcagaag cccaggttgc tcttgttctc
781 tccttctgtg gttcatctgg gggtccccct atcggtgggg gtgcagctcc aggatgtgcc
841 ccgaggacag gtagtgaaag gatcagtgtt cctgagaaac ccatctcgta ataatgtccc
901 ctgctcccca aaggtggact tcacccttag ctcagaaaga gacttcgcac tcctcagtct
961 ccaggtgccc ttgaaagatg cgaagagctg tggcctccat caactcctca gaggccctga
1021 ggtccagctg gtggcccatt cgccatggct aaaggactct ctgtccagaa cgacaaacat
1081 ccagggtatc aacctgctct tctcctctcg ccgggggcac ctctttttgc agacggacca
1141 gcccatttac aaccctggcc agcgggttcg gtaccgggtc tttgctctgg atcagaagat
1201 gcgcccgagc actgacacca tcacagtcat ggtggagaac tctcacggcc tccgcgtgcg
1261 gaagaaggag gtgtacatgc cctcgtccat cttccaggat gactttgtga tcccagacat
1321 ctcagagcca gggacctgga agatctcagc ccgattctca gatggcctgg aatccaacag
1381 cagcacccag tttgaggtga agaaatatgt ccttcccaac tttgaggtga agatcacccc
1441 tggaaagccc tacatcctga cggtgccagg ccatcttgat gaaatgcagt tagacatcca
1501 ggccaggtac atctatggga agccagtgca gggggtggca tatgtgcgct ttgggctcct
1561 agatgaggat ggtaagaaga ctttctttcg ggggctggag agtcagacca agctggtgaa
1621 tggacagagc cacatttccc tctcaaaggc agagttccag gacgccctgg agaagctgaa
1681 tatgggcatt actgacctcc aggggctgcg cctctacgtt gctgcagcca tcattgagta
1741 tccaggtggg gagatggagg aggcagagct cacatcctgg tattttgtgt catctccctt
1801 ctccttggat cttagcaaga ccaagcgaca ccttgtgcct ggggccccct tcctgctgca
1861 ggccttggtc cgtgagatgt caggctcccc agcttctggc attcctgtca aagtttctgc
1921 cacggtgtct tctcctgggt ctgttcctga agtccaggac attcagcaaa acacagacgg
1981 gagcggccaa gtcagcattc caataattat ccctcagacc atctcagagc tgcagctctc
2041 agtatctgca ggctccccac atccagcgat agccaggctc actgtggcag ccccaccttc
2101 aggaggcccc gggtttctgt ctattgagcg gccggattct cgacctcctc gtgttgggga
2161 cactctgaac ctgaacttgc gagccgtggg cagtggggcc accttttctc attactacta
2221 catgatccta tcccgagggc agatcgtgtt catgaatcga gagcccaaga ggaccctgac
2281 ctcggtctcg gtgtttgtgg accatcacct ggcaccctcc ttctactttg tggccttcta
2341 ctaccatgga gaccacccag tggccaactc cctgcgagtg gatgtccagg ctggggcctg
2401 cgagggcaag ctggagctca gcgtggacgg tgccaagcag taccggaacg gggagtccgt
2461 gaagctccac ttagaaaccg actccctagc cctggtggcg ctgggagcct tggacacagc
2521 tctgtatgct gcaggcagca agtcccacaa gcccctcaac atgggcaagg tctttgaagc
2581 tatgaacagc tatgacctcg gctgtggtcc tgggggtggg gacagtgccc ttcaggtgtt
2641 ccaggcagcg ggcctggcct tttctgatgg agaccagtgg accttatcca gaaagagact
2701 aagctgtccc aaggagaaga caacccggaa aaagagaaac gtgaacttcc aaaaggcgat
2761 taatgagaaa ttgggtcagt atgcttcccc gacagccaag cgctgctgcc aggatggggt
2821 gacacgtctg cccatgatgc gttcctgcga gcagcgggca gcccgcgtgc agcagccgga
2881 ctgccgggag cccttcctgt cctgctgcca atttgctgag agtctgcgca agaagagcag
2941 ggacaagggc caggcgggcc tccaacgagc cctggagatc ctgcaggagg aggacctgat
3001 tgatgaggat gacattcccg tgcgcagctt cttcccagag aactggctct ggagagtgga
3061 aacagtggac cgctttcaaa tattgacact gtggctcccc gactctctga ccacgtggga
3121 gatccatggc ctgagcctgt ccaaaaccaa aggcctatgt gtggccaccc cagtccagct
3181 ccgggtgttc cgcgagttcc acctgcacct ccgcctgccc atgtctgtcc gccgctttga
3241 gcagctggag ctgcggcctg tcctctataa ctacctggat aaaaacctga ctgtgagcgt
3301 ccacgtgtcc ccagtggagg ggctgtgcct ggctgggggc ggagggctgg cccagcaggt
3361 gctggtgcct gcgggctctg cccggcctgt tgccttctct gtggtgccca cggcagccgc
3421 cgctgtgtct ctgaaggtgg tggctcgagg gtccttcgaa ttccctgtgg gagatgcggt
3481 gtccaaggtt ctgcagattg agaaggaagg ggccatccat agagaggagc tggtctatga
3541 actcaacccc ttggaccacc gaggccggac cttggaaata cctggcaact ctgatcccaa
3601 tatgatccct gatggggact ttaacagcta cgtcagggtt acagcctcag atccattgga
3661 cactttaggc tctgaggggg ccttgtcacc aggaggcgtg gcctccctct tgaggcttcc
3721 tcgaggctgt ggggagcaaa ccatgatcta cttggctccg acactggctg cttcccgcta
3781 cctggacaag acagagcagt ggagcacact gcctcccgag accaaggacc acgccgtgga
3841 tctgatccag aaaggctaca tgcggatcca gcagtttcgg aaggcggatg gttcctatgc
3901 ggcttggttg tcacgggaca gcagcacctg gctcacagcc tttgtgttga aggtcctgag
3961 tttggcccag gagcaggtag gaggctcgcc tgagaaactg caggagacat ctaactggct
4021 tctgtcccag cagcaggctg acggctcgtt ccaggacctc tctccagtga tacataggag
4081 catgcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
4141 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
4201 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4261 tcactgaggc cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagctg
4321 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4381 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4441 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
4501 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4561 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
4621 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4681 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
4741 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
4801 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
4861 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
4921 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
4981 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
5041 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
5101 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
5161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
5221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
5281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
5341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
5461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
5521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
5581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
5641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
5701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
6001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
6061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
6121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
6181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
6241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
6301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
6481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
6541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
6601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6901 ttttgctggc cttttgctca catgt
//
