pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

LOCUS        pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE           9530 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes dCas9(N) fused to VEGFR2.
ACCESSION   .
VERSION     .
KEYWORDS    pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..805
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             3643..3669
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3685..5286
                     /codon_start=1
                     /product="N-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the D10A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMR"
     CDS             5317..5373
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5380..5399)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5876..5896
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6013..6033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6431..6442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6573..6590)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6579..6786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6795..6935
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6795..6924
                     /label=AAV2 ITR
     rep_origin      7010..7465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7097..7116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7307..7328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7482..7501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7601..7623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7661..7679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7747..7851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7852..8712
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8070..8089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8883..9471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9372..9391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg
      601 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      661 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agctgagcaa
      721 gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt acctggagca
      781 cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatgcagag caaggtgctg
      841 ctggccgtcg ccctgtggct ctgcgtggag acccgggccg cctctgtggg tttgcctagt
      901 gtttctcttg atctgcccag gctcagcata caaaaagaca tacttacaat taaggctaat
      961 acaactcttc aaattacttg caggggacag agggacttgg actggctttg gcccaataat
     1021 cagagtggca gtgagcaaag ggtggaggtg actgagtgca gcgatggcct cttctgtaag
     1081 acactcacaa ttccaaaagt gatcggaaat gacactggag cctacaagtg cttctaccgg
     1141 gaaactgact tggcctcggt catttatgtc tatgttcaag attacagatc tccatttatt
     1201 gcttctgtta gtgaccaaca tggagtcgtg tacattactg agaacaaaaa caaaactgtg
     1261 gtgattccat gtctcgggtc catttcaaat ctcaacgtgt cactttgtgc aagataccca
     1321 gaaaagagat ttgttcctga tggtaacaga atttcctggg acagcaagaa gggctttact
     1381 attcccagct acatgatcag ctatgctggc atggtcttct gtgaagcaaa aattaatgat
     1441 gaaagttacc agtctattat gtacatagtt gtcgttgtag ggtataggat ttatgatgtg
     1501 gttctgagtc cgtctcatgg aattgaacta tctgttggag aaaagcttgt cttaaattgt
     1561 acagcaagaa ctgaactaaa tgtggggatt gacttcaact gggaataccc ttcttcgaag
     1621 catcagcata agaaacttgt aaaccgagac ctaaaaaccc agtctgggag tgagatgaag
     1681 aaatttttga gcaccttaac tatagatggt ataacccgga gtgaccaagg attgtacacc
     1741 tgtgcagcat ccagtgggct gatgaccaag aagaacagca catttgtcag ggtccatgaa
     1801 aaaccttttg ttgcttttgg aagtggcatg gaatctctgg tggaagccac ggtgggggag
     1861 cgtgtcagaa tccctgcgaa gtaccttggt tacccacccc cagaaataaa atggtataaa
     1921 aatggaatac cccttgagtc caatcacaca attaaagcgg ggcatgtact gacgattatg
     1981 gaagtgagtg aaagagacac aggaaattac actgtcatcc ttaccaatcc catttcaaag
     2041 gagaagcaga gccatgtggt ctctctggtt gtgtatgtcc caccccagat tggtgagaaa
     2101 tctctaatct ctcctgtgga ttcctaccag tacggcacca ctcaaacgct gacatgtacg
     2161 gtctatgcca ttcctccccc gcatcacatc cactggtatt ggcagttgga ggaagagtgc
     2221 gccaacgagc ccagccaagc tgtctcagtg acaaacccat acccttgtga agaatggaga
     2281 agtgtggagg acttccaggg aggaaataaa attgaagtta ataaaaatca atttgctcta
     2341 attgaaggaa aaaacaaaac tgtaagtacc cttgttatcc aagcggcaaa tgtgtcagct
     2401 ttgtacaaat gtgaagcggt caacaaagtc gggagaggag agagggtgat ctccttccac
     2461 gtgaccaggg gtcctgaaat tactttgcaa cctgacatgc agcccactga gcaggagagc
     2521 gtgtctttgt ggtgcactgc agacagatct acgtttgaga acctcacatg gtacaagctt
     2581 ggcccacagc ctctgccaat ccatgtggga gagttgccca cacctgtttg caagaacttg
     2641 gatactcttt ggaaattgaa tgccaccatg ttctctaata gcacaaatga cattttgatc
     2701 atggagctta agaatgcatc cttgcaggac caaggagact atgtctgcct tgctcaagac
     2761 aggaagacca agaaaagaca ttgcgtggtc aggcagctca cagtcctaga gcgtgtggca
     2821 cccacgatca caggaaacct ggagaatcag acgacaagta ttggggaaag catcgaagtc
     2881 tcatgcacgg catctgggaa tccccctcca cagatcatgt ggtttaaaga taatgagacc
     2941 cttgtagaag actcaggcat tgtattgaag gatgggaacc ggaacctcac tatccgcaga
     3001 gtgaggaagg aggacgaagg cctctacacc tgccaggcat gcagtgttct tggctgtgca
     3061 aaagtggagg catttttcat aatagaaggt gcccaggaaa agacgaactt ggaaatcatt
     3121 attctagtag gcacggcggt gattgccatg ttcttctggc tacttcttgt catcatcggc
     3181 ggtggctcgg gtggtggctc gggagaaagc ttgtttaagg ggccgcgtga ttacaacccg
     3241 atatcgagca ccatttgtca tttgacgaat gaatctgatg ggcacacaac atcgttgtat
     3301 ggtattggat ttggtccctt catcattaca aacaagcact tgtttagaag aaataatgga
     3361 acactgttgg tccaatcact acatggtgta ttcaaggtca agaacaccac gactttgcaa
     3421 caacacctca ttgatgggag ggacatgata attattcgca tgcctaagga tttcccacca
     3481 tttcctcaaa agctgaaatt tagagagcca caaagggaag agcgcatatg tcttgtgaca
     3541 accaacttcc aaactggcgg tggctcgcta gatttagcta gcttaatcct cggcaagctt
     3601 ggcgaaaacc tgtattttca gctaggcgga ggcagcacta gttacccata cgacgtacca
     3661 gattacgctg gaggttctgg atccgacaag aagtacagca tcggcctggc catcggcacc
     3721 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
     3781 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     3841 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     3901 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     3961 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     4021 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     4081 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     4141 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     4201 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     4261 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     4321 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     4381 aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     4441 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     4501 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     4561 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     4621 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     4681 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     4741 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     4801 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     4861 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     4921 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     4981 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     5041 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     5101 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     5161 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     5221 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     5281 atgagaggtg gcggtggctc gggtaccgga tctggagcca ccaatttttc tctgctgaag
     5341 caggctggtg atgttgaaga aaaccctgga cctgaattca tgaccgagta caagcccacg
     5401 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5461 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5521 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5581 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5641 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5701 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5761 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5821 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5881 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5941 ccgcgcacct ggtgcatgac ccgcaagggg taactaagta aggatcgatc caagatatcg
     6001 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6061 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6121 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6181 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6241 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6301 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6361 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6421 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6481 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6541 ctccccgcca attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     6601 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6661 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6721 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc
     6781 tggggagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     6841 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     6901 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     6961 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     7021 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7081 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7141 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7201 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     7261 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7321 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     7381 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7441 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     7501 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7561 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7621 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7681 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7741 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7801 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7861 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7921 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7981 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8041 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8101 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8161 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8221 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8281 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8341 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8401 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8461 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8521 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc
     8581 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8641 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8701 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8761 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8821 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8881 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8941 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9001 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9061 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9121 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9181 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9241 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9301 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9361 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9421 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9481 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
//