pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NCKAP5-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NCKAP5-FLAG                7218 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3932
                     /codon_start=1
                     /label=NCKAP5
                     /translation="VSSSSSSSSPAKSHNSPHGCQSAHEKGLKTRLPVGLKVLMKSPQL
                     LRKSSTVPGKHEKDSLNEASKSSVAVNKSKPEDSKNPASMEITAGERNVTLPDSQAQGS
                     LADGLPLETALQEPLESSIPGSDGRDGVDNRSMRRSLSSSKPHLKPALGMNGAKARSHS
                     FSTHSGDKPSTPPIEGSGKVRTQIITNTAERGNSLTRQNSSTESSPNKAPSAPMLESLP
                     SVGRPSGHPSSGKGSLGSSGSFSSQHGSPSKLPLRIPPKSEGLLIPPGKEDQQAFTQGE
                     CPSANVAVLGEPGSDRRSCPPTPTDCPEALQSPGRTQHPSTFETSSTSKLETSGRHPDA
                     SATATDAVSSEAPLSPTIEEKVMLCIQENVEKGQVQTKPTSVEAKQKPGPSFASWFGFR
                     KSRLPALSSRKMDISKTKVEKKDAKVLGFGNRQLKSERKKEKKKPELQCETENELIKDT
                     KSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHSPVACSTKDTFMTELLNRVDKKAAP
                     QTESGSSNASCRNVLKGSSQGSCLIGSSISTQGNHKKNMKIKADMEVPKDSLVKEANEN
                     LQEDEDDAVADSVFQSHIIESNCQMRTLDSGIGTFPLPDSGNRSTGRYLCQPDSPEDAE
                     PLLPLQSALSAVSSMRAQTLEREVPSSTDGQRPADSAIVHSTSDPIMTARGMRPLQSRL
                     PKPASSGKVSSQKQNEAEPRPQTCSSFGYAEDPMASQPLPDWGSEVAATGTQDKAPRMC
                     TYSASGGSNSDSDLDYGDNGFGAGRGQLVKALKSAAPEIETT"
     misc_feature    2238..2243
                     /label=SDS
     CDS             3938..4003
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4007..4438
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4151..4156
                     /label=SDS
     repeat_region   4481..4621
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4481..4610
                     /label=AAV2 ITR
     rep_origin      4696..5151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5433..5537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5538..6398
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6569..7157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtca gcagttcctc
     1561 atcatcctca tcacccgcca aaagccataa cagccctcat ggttgtcaaa gtgctcatga
     1621 gaaaggactg aaaactcgcc ttccagtggg actcaaagtg ctcatgaagt ctccccagct
     1681 gctcaggaaa agttccaccg tgccagggaa acatgaaaaa gacagtttaa atgaagcctc
     1741 caaaagttcc gtggctgtga acaagtctaa gccagaggac tccaagaatc cagcaagcat
     1801 ggagatcaca gcgggtgaaa gaaatgtgac cctaccggat tcacaagcac agggcagttt
     1861 agctgatggg cttcccctgg aaacagcact acaagagcca ttggaaagta gcatccctgg
     1921 gagtgatgga agggatgggg tagataatag atccatgaga agatcccttt cctccagcaa
     1981 accacaccta aaaccagctc tgggtatgaa tggcgccaaa gcccgcagcc acagcttcag
     2041 tacacactca ggagacaagc cttctacgcc ccccatcgaa gggtcaggca aagtccgcac
     2101 tcagatcatt accaataccg ccgagagagg caattctctt acccggcaga actcttccac
     2161 ggaaagctct cccaacaagg ccccttctgc tcccatgttg gagagtctcc ccagtgttgg
     2221 gaggccctcg gggcacccct cctccgggaa gggctccctg gggagctcag gcagcttcag
     2281 cagtcagcat gggagcccaa gtaagttgcc tttgaggatc cctccaaagt ctgagggact
     2341 cctcatccca cctggaaagg aagaccagca ggccttcacc cagggagagt gccccagtgc
     2401 caatgtggct gtacttgggg aaccaggcag tgaccgccgc agttgcccac ccaccccaac
     2461 agactgccct gaagccctgc agagcccagg gaggactcag catccaagca cttttgaaac
     2521 aagcagtaca tccaagctag aaacttctgg aaggcatcca gatgcctctg caaccgcgac
     2581 tgatgctgtg agttcagaag cccccctctc acccacaatc gaagaaaagg tcatgttgtg
     2641 cattcaggaa aatgtggaaa agggccaagt gcaaacaaag cccacctctg tggaagcaaa
     2701 gcagaagcct gggccttctt ttgccagctg gtttggtttt cggaagagta gacttccagc
     2761 tctgagtagc aggaaaatgg acatctccaa aaccaaagta gaaaagaaag atgcaaaagt
     2821 cttggggttt ggaaacagac aactaaaatc agaaagaaaa aaagagaaaa agaagcctga
     2881 actacagtgt gagacagaaa atgagcttat caaggacacc aagtcagcag ataatccaga
     2941 tggcggttta caaagcaaaa ataatcggag aacaccacaa gacatttaca accaactgaa
     3001 gattgaacca aggaatagac acagccctgt tgcatgttca acgaaagaca ccttcatgac
     3061 ggaactcttg aacagagttg ataagaaagc agctccacag acagaaagtg gatcaagtaa
     3121 tgcttcctgc aggaatgtgt taaagggcag ttctcagggc tcctgtctca tcggcagctc
     3181 tatcagtact caaggaaacc acaagaaaaa catgaaaatc aaagccgata tggaagtacc
     3241 aaaagactcc ctggtaaaag aggcaaatga aaacttgcaa gaggatgaag acgatgcagt
     3301 tgcagattct gtatttcaga gccacatcat agaatccaac tgccagatga gaacattgga
     3361 cagtgggatc ggaacctttc cactcccaga ctcgggaaat cgctcgacag gacgctacct
     3421 atgccagcca gactccccag aggacgctga gcctctcctg cctctccagt cagccctttc
     3481 tgcagtttct tccatgagag cccaaaccct tgaacgtgaa gtgccttcct ccacagacgg
     3541 ccagcgccct gcagatagcg ccattgttca ttccacatcc gaccccatca tgaccgccag
     3601 agggatgagg cctcttcaga gccgcctccc caaaccagct tcctcaggaa aagtcagttc
     3661 ccaaaagcag aatgaagcag agccaaggcc tcagacatgc tcatcattcg gatatgctga
     3721 agacccaatg gcaagccagc cgcttccaga ctgggggagt gaagttgctg ccaccgggac
     3781 ccaggacaag gcacccagaa tgtgtacgta ctctgccagc ggtggcagta atagtgacag
     3841 tgacctggac tatggagata atggttttgg agctggaagg ggacagttag tgaaagcact
     3901 gaagagcgct gccccagaaa ttgagacaac ttgtacagac tacaaagacc atgacggtga
     3961 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
     4021 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4081 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4141 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
     4201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4261 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
     4321 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4381 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     4441 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     4501 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4561 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4621 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4681 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4741 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     4801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     5041 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     5101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5161 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5221 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5281 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5341 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5401 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5461 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5521 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5581 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5641 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5701 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5761 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5821 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5881 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5941 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6001 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6061 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6121 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6181 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6241 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6301 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6361 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6421 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6481 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6541 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6601 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6661 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6721 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6781 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6901 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6961 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7081 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7141 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7201 ggccttttgc tcacatgt
//