| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001556 |
| 产品用途: | 3' AAVLINK plasmid for LAMB1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
LAMB1
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-LAMB1-FLAG 6781 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6781)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6781
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3494
/codon_start=1
/label=LAMB1
/translation="GH*DATV*PVHGPVCLR*GC*GSTL*QVHARVLGGLP*LHTLPPV
LCSLGCDHCRADQQDTQIPGESQGLEDQWCDRALP*DCGLGGEESQRDKRHPGAEPRSR
ATEKHWESL*GSRETD*RCYRNDGSSRSEII*HNFPKQQHSQRTGFSTDRSRKPRQHCE
RTC*TTGIYQKLRYSGCLG*HYQVFPDVS*GRGEGECLHHRTQQHCGAVSPHERQSRRR
DDGARIPVQGKTRGAGSPP**TGRQATKPRPFSRCRNDLWNTPRGLLFRD*MWRAKLQN
*RRREEVWGAWLWWSGYCCTQRLAESHGLGPRCPECPG*SGTALQDGL*SKTEGR*GKT
KC*RHSVEDKCYQRKNGQEQ*GAEKSNQANQKLFDPG*C*FGQH*SSC**SIENGDA*H
PTAVTELDRRYT*TS*KPFSSRGYSSA*CC*HCQS*DVVRRS*KSKQKCNRC*SHCRYG
KGSSGRSRKGPGRSREGN*TSR*RHSRNPEPANFD*V*NSSF*GNLVQRVPAHQRVREE
CGRT*AESCPKLRGGRIY*KSSIYCEAKCRRC*EDFRW*T**KV*KSRKFNCQKN*RVS
*CQKESRNATK*SKNSFSSSK*QAATAQRFRKKI*RQSKILRR*SSRISKTGRRSPFTP
KGYKPESCCV*HML"
misc_feature 2264..2269
/label=SDS
misc_feature 2347..2352
/label=SDS
CDS 3501..3566
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3570..4001
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3714..3719
/label=SDS
repeat_region 4044..4184
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4044..4173
/label=AAV2 ITR
rep_origin 4259..4714
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4996..5100
/gene="bla"
/label=AmpR promoter
CDS 5101..5961
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6132..6720
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc attgagacgc
1561 cacagtgtga ccagtccacg ggccagtgtg tctgcgttga gggtgttgag ggtccacgct
1621 gtgacaagtg cacgcgaggg tactcggggg tcttccctga ctgcacaccc tgccaccagt
1681 gctttgctct ctgggatgtg atcattgccg agctgaccaa caggacacac agattcctgg
1741 agaaagccaa ggccttgaag atcagtggtg tgatcgggcc ttaccgtgag actgtggact
1801 cggtggagag gaaagtcagc gagataaaag acatcctggc gcagagcccc gcagcagagc
1861 cactgaaaaa cattgggaat ctctttgagg aagcagagaa actgattaaa gatgttacag
1921 aaatgatggc tcaagtagaa gtgaaattat ctgacacaac ttcccaaagc aacagcacag
1981 ccaaagaact ggattctcta cagacagaag ccgaaagcct agacaacact gtgaaagaac
2041 ttgctgaaca actggaattt atcaaaaact cagatattcg gggtgccttg gatagcatta
2101 ccaagtattt ccagatgtct cttgaggcag aggagagggt gaatgcctcc accacagaac
2161 ccaacagcac tgtggagcag tcagccctca tgagagacag agtagaagac gtgatgatgg
2221 agcgagaatc ccagttcaag gaaaaacaag aggagcaggc tcgcctcctt gatgaactgg
2281 caggcaagct acaaagccta gacctttcag ccgctgccga aatgacctgt ggaacacccc
2341 caggggcctc ctgttccgag actgaatgtg gcgggccaaa ctgcagaact gacgaaggag
2401 agaggaagtg tggggggcct ggctgtggtg gtctggttac tgttgcacac aacgcctggc
2461 agaaagccat ggacttggac caagatgtcc tgagtgccct ggctgaagtg gaacagctct
2521 ccaagatggt ctctgaagca aaactgaggg cagatgaggc aaaacaaagt gctgaagaca
2581 ttctgttgaa gacaaatgct accaaagaaa aaatggacaa gagcaatgag gagctgagaa
2641 atctaatcaa gcaaatcaga aactttttga cccaggatag tgctgatttg gacagcattg
2701 aagcagttgc taatgaagta ttgaaaatgg agatgcctag caccccacag cagttacaga
2761 acttgacaga agatatacgt gaacgagttg aaagcctttc tcaagtagag gttattcttc
2821 agcatagtgc tgctgacatt gccagagctg agatgttgtt agaagaagct aaaagagcaa
2881 gcaaaagtgc aacagatgtt aaagtcactg cagatatggt aaaggaagct ctggaagaag
2941 cagaaaaggc ccaggtcgca gcagagaagg caattaaaca agcagatgaa gacattcaag
3001 gaacccagaa cctgctaact tcgattgagt ctgaaacagc agcttctgag gaaaccttgt
3061 tcaacgcgtc ccagcgcatc agcgagttag agaggaatgt ggaagaactt aagcggaaag
3121 ctgcccaaaa ctccggggag gcagaatata ttgaaaaagt agtatatact gtgaagcaaa
3181 gtgcagaaga tgttaagaag actttagatg gtgaacttga tgaaaagtat aaaaaagtag
3241 aaaatttaat tgccaaaaaa actgaagagt cagctgatgc cagaaggaaa gccgaaatgc
3301 tacaaaatga agcaaaaact cttttagctc aagcaaatag caagctgcaa ctgctcaaag
3361 atttagaaag aaaatatgaa gacaatcaaa gatacttaga agataaagct caagaattag
3421 caagactgga aggagaagtc cgttcactcc taaaggatat aagccagaaa gttgctgtgt
3481 atagcacatg cttgtgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
3541 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
3601 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
3661 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
3721 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
3781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
3841 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
3901 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
3961 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg
4021 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
4081 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
4141 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
4201 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
4261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4321 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4441 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4561 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
4621 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4681 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4741 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4801 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4861 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
4921 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
4981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
5041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
5101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
5161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
5221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
5581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
5881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
5941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
6001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
6061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
6121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
6181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
6301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6781 t
//
