pAAVLINKv1-pCALM1-Cre-3'-CCDC88C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-CCDC88C-FLAG            7231 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3944
                     /codon_start=1
                     /label=CCDC88C
                     /translation="ELGERHGDMLKRKAELEEREKVLTTEREALQQEQRTNALAMGENQ
                     RLRGELDRVNFLHHQLKGEYEELHAHTKELKTSLNNAQLELNRWQARFDELKEQHQTMD
                     ISLTKLDNHCELLSRLKGNLEEENHHLLSQIQLLSQQNQMLLEQNMENKEQYHEEQKQY
                     IDKLNALRRHKEKLEEKIMDQYKFYDPPPKKKNHWIGAKALVKLIKPKKEGSRERLKST
                     VDSPPWQLESSDPASPAASQPLRSQAENPDTPALGSNCAEERDAHNGSVGKGPGDLKPK
                     RGSPHRGSLDRTDASTDLAMRSWPSELGSRTCSTSATTTAPSNSTPIARHPGRTKGYNS
                     DDNLCEPSLEFEVPNHRQYVSRPSSLESSRNTSSNSSPLNLKGSSEQLHGRSESFSSED
                     LIPSRDLATLPREASTPGRNALGRHEYPLPRNGPLPQEGAQKRGTAPPYVGVRPCSASP
                     SSEMVTLEEFLEESNRSSPTHDTPSCRDDLLSDYFRKASDPPAIGGQPGPPAKKEGAKM
                     PTNFVAPTVKMAAPTSEGRPLKPGQYVKPNFRLTEAEAPPSVAPRQAQPPQSLSLGRPR
                     QAPVPPASHAPASRSASLSRAFSLASADLLRASGPEACKQESPQKLGAPEALGGRETGS
                     HTLQSPAPPSSHSLARERTPLVGKAGSSCQGPGPRSRPLDTRRFSLAPPKEERLAPLHQ
                     SATAPAIATAGAGAAAAGSGSNSQLLHFSPAAAPAARTKPKAPPRSGEVATITPVRAGL
                     SLSEGDGVPGQGCSEGLPAKSPGRSPDLAPHPGRALEDCSRGSVSKSSPASPEPGGDPQ
                     TVWYEYGCV"
     misc_feature    1871..1876
                     /label=SDS
     misc_feature    2049..2054
                     /label=SDS
     misc_feature    2788..2793
                     /label=SDS
     misc_feature    3256..3261
                     /label=SDS
     misc_feature    3535..3540
                     /label=SDS
     CDS             3951..4016
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4020..4451
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4164..4169
                     /label=SDS
     repeat_region   4494..4634
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4494..4623
                     /label=AAV2 ITR
     rep_origin      4709..5164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5446..5550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5551..6411
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6582..7170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggagctcggg gagaggcacg
     1501 gtgacatgct gaagcgcaag gcggagctgg aggagcggga gaaggtcttg accactgagc
     1561 gagaggcgct gcagcaggag cagaggacaa acgccctcgc catgggcgag aaccagaggc
     1621 tgcggggcga gctggacagg gtcaatttcc tgcaccacca gctgaagggg gagtacgagg
     1681 agctgcacgc ccacaccaag gagctgaaaa cctcactgaa caacgcgcag ctggagctca
     1741 accgctggca ggcccgcttc gacgagctga aggagcagca ccagaccatg gacatctcgc
     1801 tgaccaagct ggacaaccac tgtgagctgc tctcccgtct caaggggaac ttggaggaag
     1861 aaaatcatca cctcctgagc cagatccagc tgttgagcca gcagaaccag atgcttctgg
     1921 agcagaacat ggagaacaag gagcagtacc atgaggagca gaagcagtac atagacaaat
     1981 taaatgcctt acgaagacat aaggaaaagc tggaagaaaa aatcatggat caatacaagt
     2041 tctatgatcc tcctccaaag aagaagaacc actggattgg agccaaagcc ttagtcaaac
     2101 tcatcaaacc aaagaaagag ggttcgaggg aacgcttaaa atccaccgtg gacagccctc
     2161 cctggcagct ggagtcctca gaccccgcct cgccggcggc ctctcagccg ctcagatcac
     2221 aggccgagaa ccccgacacc cccgcactgg gctccaactg tgcagaagag cgcgacgccc
     2281 acaacgggtc tgtggggaaa ggccctgggg atctaaaacc aaagcgaggc tccccacaca
     2341 gaggcagcct tgaccgcaca gatgcctcca ccgatctggc catgaggtcc tggccctcgg
     2401 agctgggctc ccggacttgc tcaacttcag ccaccactac agccccttcc aactccaccc
     2461 ccatcgcccg gcacccaggc cgcaccaaag gctataactc agatgacaac ctctgtgagc
     2521 catccctgga gtttgaggtc cccaaccaca ggcagtacgt gtcgcggcca agtagcttag
     2581 agagcagtag aaacacatcc agcaacagct cacctcttaa cctaaaaggc tcctccgagc
     2641 agctccatgg ccggtctgag agcttcagca gcgaagacct gatccccagc agggacctgg
     2701 ccactttgcc ccgggaagcc agcacaccgg gacgcaacgc cctcggccgc cacgagtacc
     2761 ccttgcctcg gaacgggcct ctcccacagg agggtgccca gaagaggggc acagcccctc
     2821 cctacgtcgg agtgcggccc tgctcggcct cccccagcag tgagatggtc accttggagg
     2881 agttcctgga ggagagcaac cgcagctccc ccacccatga cactcccagt tgccgggatg
     2941 acctgctgag tgactacttc cgaaaggcca gcgatccccc agccatcgga ggccaaccag
     3001 gaccacctgc caagaaagaa ggggccaaga tgcccaccaa ctttgtggcc cccaccgtca
     3061 aaatggccgc ccccacctcg gaggggaggc cgctgaagcc cgggcagtac gtaaagccaa
     3121 acttcagact gactgaggcc gaggccccac ccagcgtggc cccgagacag gcccagcctc
     3181 cccagagcct gtctctgggc agaccccggc aggctccggt gcccccagct tcccatgcac
     3241 ctgccagccg cagtgcctcc ttgagccggg ccttcagcct ggcctcagct gaccttctcc
     3301 gggccagcgg gccagaggcc tgcaaacagg agtcccctca gaagctgggg gctcctgagg
     3361 ccttaggggg cagagagaca ggcagccaca ccctgcaaag ccccgcaccc cccagctccc
     3421 atagcctggc ccgggagcgg accccacttg tgggaaaggc tggcagctcc tgtcagggcc
     3481 caggtccccg cagccggccg ctggacacga ggcgcttctc cctggctccc ccaaaggagg
     3541 agaggctggc ccccctgcat cagtctgcca cagcccccgc cattgccact gcaggtgctg
     3601 gtgctgctgc tgctggcagt ggcagcaact cccagctcct gcacttctca cctgctgcag
     3661 ccccggctgc caggaccaag cccaaggcgc ccccacgctc aggggaggtg gccaccatca
     3721 cccctgtccg ggcagggctc agcctctcag agggagacgg ggtcccgggg cagggctgca
     3781 gtgaggggct tccggccaag agcccaggtc ggtctcccga tttggctccc caccccggcc
     3841 gggccctgga ggactgcagt cgagggagcg tctcaaagag cagtccggcc tccccggagc
     3901 ccggcgggga tccgcagacc gtgtggtatg agtacggctg tgtgtgtaca gactacaaag
     3961 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac
     4021 tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc
     4201 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4261 caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta
     4321 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4381 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     4441 aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc
     4501 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4561 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4621 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4681 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4741 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     4801 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4861 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4921 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4981 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5041 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     5101 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5161 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5221 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5281 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5341 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5401 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5521 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5581 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5641 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5701 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5761 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5821 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5881 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5941 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6001 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6061 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6121 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6181 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6241 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6301 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6361 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6421 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7201 ctggcctttt gctggccttt tgctcacatg t
//