| 质粒名称: | pAAVLINK-EF1a-HA-5'-RERE |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001374 |
| 产品用途: | 5' AAVLINK plasmid for RERE expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RERE |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-RERE载体质粒基本信息
pAAVLINK-EF1a-HA-5'-RERE质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-RERE载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-RERE质粒载体简介
pAAVLINK-EF1a-HA-5'-RERE质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-RERE 6834 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6834)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6834
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3974
/codon_start=1
/label=RERE
/translation="TADKDKDKDKEKDRDRDRDREREKRDKARESENSRPRRSCTLEGG
AKNYAESDHSEDEDNDNNSATAEESTKKNKKKPPKKKSRYERTDTGEITSYITEDDVVY
RPGDCVYIESRRPNTPYFICSIQDFKLVHNSQACCRSPTPALCDPPACSLPVASQPPQH
LSEAGRGPVGSKRDHLLMNVKWYYRQSEVPDSVYQHLVQDRHNENDSGRELVITDPVIK
NRELFISDYVDTYHAAALRGKCNISHFSDIFAAREFKARVDSFFYILGYNPETRRLNST
QGEIRVGPSHQAKLPDLQPFPSPDGDTVTQHEELVWMPGVNDCDLLMYLRAARSMAAFA
GMCDGGSTEDGCVAASRDDTTLNALNTLHESGYDAGKALQRLVKKPVPKLIEKCWTEDE
VKRFVKGLRQYGKNFFRIRKELLPNKETGELITFYYYWKKTPEAASSRAHRRHRRQAVF
RRIKTRTASTPVNTPSRPPSSEFLDLSSASEDDFDSEDSEQELKGYACRHCFTTTSKDW
HHGGRENILLCTDCRIHFKKYGELPPIEKPVDPPPFMFKPVKEEDDGLSGKHSMRTRRS
RGSMSTLRSGRKKQPASPDGRTSPINEDIRSSGRNSPSAASTSSNDSKAETVKKSAKKV
KEEASSPLKSNKRQREKVASDTEEADRTSSKKTKTQEISRPNSPSEGEGESSDSRSVND
EGSSDPKDIDQDNRSTSPSIPSPQDNESDSDSSAQQQMLQAQPPALQAPTGVTPAPSSA
PPGTPQLPTPGPTPSATAVPPQGSPTASQAPNQPQAPTAPVPHTHIQQAPALHPQRPPS
PHPPPHPSPHPPLQPLTGSAGQPSAPSHAQPPLHGQGPPGPHSLQAGPLLQHPGPPQPF
GLPPQASQGQAPLGTSPAAAYPHTSLQLPASQSALQSQQPPREQPLPPAPLAMPHIKPP
PTTPIPQLPAPQAHKHPPHLSGPSPFSMNANLPPPPALKPLSSLSTHHPPSAHPPPLQL
MPQSQPLPSSPAQPPGLTQSQNLPPPPASHPPTGLHQVAPQPPFAQHPFVPGGPPPITP
PTCPSTSTPPAGPGTSAQPPCSGAAAS"
misc_feature 877..882
/label=SDS
misc_feature 1574..1579
/label=SDS
RBS 1722..1730
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 1723..1728
/label=SDS
misc_feature 2123..2128
/label=SDS
misc_feature 2655..2660
/label=SDS
misc_feature 2721..2726
/label=SDS
misc_feature 2997..3002
/label=SDS
misc_feature 3738..3743
/label=SDS
misc_feature 3875..3880
/label=SDS
intron 3975..4015
/label=Splicing Donor
misc_feature 4019..4052
/label=lox2272-JT1571
repeat_region 4097..4237
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4097..4226
/label=AAV2 ITR
rep_origin 4312..4767
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5049..5153
/gene="bla"
/label=AmpR promoter
CDS 5154..6014
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6185..6773
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctac agcggacaaa gacaaagaca aagacaaaga
781 gaaggaccgg gaccgagacc gggaccgaga gagagagaaa agagacaaag caagagagag
841 tgagaattca aggccacgcc ggagctgtac cttggaagga ggagccaaaa attatgctga
901 gagtgatcac agtgaagacg aggacaatga caacaatagt gccaccgcag aggagtccac
961 gaagaagaat aagaagaaac caccgaaaaa aaagtctcgt tatgaaagga cagataccgg
1021 tgagataaca tcctacatca ctgaagatga tgtggtctac agaccaggag actgtgtgta
1081 tatcgagagt cggaggccaa acacaccgta tttcatctgt agcattcaag acttcaaact
1141 ggtccacaac tcccaggcct gttgcagatc tccaactcct gctttgtgtg accccccagc
1201 atgctctctg ccggtggcat cacagccacc acagcatctt tctgaagccg ggagagggcc
1261 tgtagggagt aagagggacc atctcctcat gaacgtcaaa tggtactacc gtcaatctga
1321 ggttccagat tctgtgtatc agcatttggt tcaggatcga cataatgaaa atgactctgg
1381 aagagaactt gtcattacag acccagttat caagaaccga gagctcttca tttctgatta
1441 cgttgacact taccatgctg ctgcccttag agggaagtgt aacatctccc atttttctga
1501 catatttgct gctagagagt ttaaagcccg agtggattca tttttctaca tattaggata
1561 taaccctgag acaaggaggc tgaacagtac ccagggggag attcgtgtcg gtcctagtca
1621 tcaggccaaa cttccagatc tgcaaccatt tccttctcca gatggtgata cagtgaccca
1681 acatgaggaa ctggtctgga tgcctggagt taacgactgt gacctcctta tgtacttgag
1741 ggcagcaagg agcatggcgg catttgcagg aatgtgtgat ggaggctcta cagaggacgg
1801 ctgtgtcgca gcctctcggg atgacaccac tctgaatgca ctgaacacac tgcatgaaag
1861 cggttacgat gctggcaaag ccctgcagcg cctggtgaag aagcctgtgc ccaagctcat
1921 cgagaagtgc tggaccgagg acgaagtgaa acgcttcgtt aagggactca ggcagtacgg
1981 gaagaacttc ttcagaatta gaaaggagct gcttcccaat aaggaaacag gggagctgat
2041 caccttctat tactattgga agaagacccc cgaagcagcc agctcccgag cccatcgtag
2101 gcaccgcagg caggccgtgt tcaggaggat taagactcgc accgcgtcca cacccgtcaa
2161 cacaccctcc agacccccgt ccagtgaatt cttggaccta agttcagcca gtgaagatga
2221 cttcgacagt gaggacagtg agcaggagct gaaggggtac gcctgccgcc actgcttcac
2281 caccacctcc aaagattggc accacggagg ccgggagaac atcctgcttt gcaccgactg
2341 tcgcatccac ttcaagaaat acggtgagct cccgcccatt gagaagcccg tggacccgcc
2401 accgtttatg ttcaaacccg tcaaggaaga ggatgatggg ctcagtggga agcatagcat
2461 gaggacacgg cggagtcggg gctcgatgtc gacactacgc agtggtcgga agaagcagcc
2521 agccagccct gatggtcgca cctcacccat caatgaagac atccgctcca gcggccggaa
2581 ctcccccagc gctgccagta cctccagcaa tgacagtaaa gcagagacag tgaagaagtc
2641 ggccaagaag gtgaaggagg aagcctcttc ccctcttaag agtaacaaac gccagcggga
2701 gaaggtggcc tctgatacgg aggaggctga caggaccagc tccaagaaga caaaaacgca
2761 ggagatcagc aggcccaact cgccatctga aggtgaggga gagagttcag acagtcgcag
2821 cgtcaacgat gagggtagca gtgaccccaa agacatcgac caggacaatc gcagcacgtc
2881 cccgagcatc cccagccccc aggacaatga gagtgactcg gactcgtcag cccagcagca
2941 gatgctgcag gcccagcccc cagccttgca ggctcccact ggggtcaccc cagctccctc
3001 ctcagctcct ccagggaccc ctcagctgcc cacgccaggg cccacgccct ctgccactgc
3061 agttccccca cagggctccc ccacggcctc ccaggcccct aaccagccac aggctcccac
3121 agcgcctgtt ccccacaccc acatccaaca ggcaccggcc ttgcaccccc agcggccgcc
3181 ctcaccgcat cccccgccgc atccctcgcc acatcccccg ctgcagcctc tgactgggtc
3241 ggcgggccag ccttctgcac cctctcatgc ccagccccca ctgcacggtc agggcccacc
3301 cggccctcac agcctgcagg ctgggcccct gctgcagcac ccaggccccc cacagccctt
3361 tggcctccct ccccaggcct cccaaggcca ggcccctctg gggacctccc cagcagcagc
3421 gtaccctcac acctccctgc agctgccagc ctctcagtca gcgctgcagt cccaacagcc
3481 tccacgggag cagcccctgc caccagcgcc cttggccatg ccccacatca agcccccgcc
3541 taccactccc atcccccagc tgccggcgcc acaggcccac aagcaccctc cccacctctc
3601 ggggccctca cccttctcca tgaatgccaa cctgcctccc cctccagccc tgaagcccct
3661 gagctccctg tccacacatc accccccgtc ggctcacccc ccacccctgc aactcatgcc
3721 tcagagccag ccattgccct cctcgcccgc ccagcccccc gggctgaccc agagccagaa
3781 cctgcccccg ccccctgcct cccacccccc tacaggcctc caccaggtgg ccccccaacc
3841 cccgtttgct cagcacccct ttgtccctgg aggccctcct cccatcaccc ctccgacctg
3901 cccctccacc tctaccccac cggcgggacc tggcacctcg gcccagccac cctgctctgg
3961 tgcggcggct tcaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
4021 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
4081 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
4141 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
4201 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
4261 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4381 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4501 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4621 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
4681 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4741 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
4801 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
4861 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
4921 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
4981 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
5041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
5101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
5161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
5221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
5281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
5341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
5401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
5461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
5521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
5581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
5641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
5701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
6001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
6061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
6121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
6181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
6241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
6301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
6361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
6421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
6481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
6541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
6601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
6661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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