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pGreenII 0800-miRNA载体质粒基本信息
pGreenII 0800-miRNA质粒图谱载体图谱和pGreenII 0800-miRNA载体序列质粒序列多克隆位点信息
pGreenII 0800-miRNA质粒载体简介
pGreenII 0800-miRNA质粒序列载体序列
LOCUS pGreenII 0800-miRNA 6706 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6706)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6706
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 542..564
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 626..971
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 982..1917
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1974..2150
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind 2314..2330
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2337..2355
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 2381..2397
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter 2428..2773
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 2783..4435
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 4512..4688
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 4698..4722
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(4813..5401)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5572..6387)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin join(6678..6706,1..407)
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
541 ttggcaggat atattgtggt gtaacgttat cgtaccccta ctccaaaaat gtcaaagata
601 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
661 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
721 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
781 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
841 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
901 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
961 tggagaggac agcccaccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
1921 tagccggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc tattttctcc
1981 ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata gggtttcgct
2041 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
2101 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc gataacatta
2161 acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
2221 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
2281 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
2341 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
2401 agctttctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
2461 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
2521 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
2581 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
2641 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
2701 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
2761 tttggagaga acacggggga ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc
2821 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
2881 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
2941 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
3001 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
3061 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
3121 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
3181 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
3241 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
3301 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
3361 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
3421 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
3481 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
3541 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
3601 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
3661 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
3721 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
3781 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
3841 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
3901 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
3961 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
4021 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
4081 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
4141 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
4201 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
4261 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
4321 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
4381 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta
4441 gagcggccgc ggatccacta gtgaattcgc tgaaatcacc agtctctctc tacaaatcta
4501 tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg
4561 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
4621 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
4681 tccagatcca ctagccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt
4741 tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
4801 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
4861 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
4921 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
4981 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
5041 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
5101 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
5161 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
5221 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
5281 gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac aaaccaccgc
5341 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
5401 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
5461 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
5521 atgaagtttt aaatcaatct aaagtatata tgtgtaacat tggtctagtg attagaaaaa
5581 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
5641 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
5701 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
5761 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
5821 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
5881 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
5941 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
6001 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
6061 tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt
6121 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
6181 catctcatct gtaacaacat tggcaacgct acctttgcca tgtttcagaa acaactctgg
6241 cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg
6301 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca
6361 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
6421 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
6481 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
6541 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
6601 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg
6661 ggcgattcag gcgatcccca tccaacagcc cgccgtcgag cgggct
//
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