pacAd5 CMV-ntLacZ

LOCUS       pacAd5 CMV-ntLacZ                9278 bp DNA     circular SYN 12-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     206..357
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        508..769
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     793..809
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             879..899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4039..4055)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4075..4209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7451..8039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8210..9070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9071..9175)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
       61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
      121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
      181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
      241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
      301 aactgaataa gaggaagtga aatctgaata attttgtgtg tactcatagc gcgtaatatt
      361 tgtctaggga gatctgcgat gtacgggcca gatatacgcg tatctgaggg gactagggtg
      421 tgtttaggcg aaaagcgggg cttcggttgt acgcggttag gagtcccctc aggatatagt
      481 agtttcgctt ttgcataggg agggggaaat gtagtcttat gcaatactct tgtagtcttg
      541 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
      601 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
      661 gacatggatt ggacgaacca ctgaattccg cattgcagag atattgtatt taagtgccta
      721 gctcgataca ataaacgcca tttgaccatt caccacattg gtgtgcacct ccaagctggt
      781 accgtttaaa ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg
      841 gggatccccg ggtaccgagc tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac
      901 cggtgggtga agaccagaaa cagcacctcg aactgagccg cgatattgcc cagcgtttca
      961 acgcgctgta tggcgagatc gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1021 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1081 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     1141 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     1201 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     1261 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     1321 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa aggccagacg
     1381 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc
     1441 ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt ttacgcgccg
     1501 gagaaaccgc tcgcggtgat ggtgctgcgc tggagtgacg gcagtatctg gaagatcagg
     1561 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac
     1621 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg
     1681 aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat
     1741 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
     1801 agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt
     1861 ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca
     1921 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc
     1981 tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc
     2041 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
     2101 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc
     2161 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
     2221 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa
     2281 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
     2341 aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt
     2401 cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc
     2461 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
     2521 aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
     2581 cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc
     2641 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
     2701 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt
     2761 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa
     2821 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg
     2881 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta
     2941 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg
     3001 aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag
     3061 tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc
     3121 gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga
     3181 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
     3241 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
     3301 gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
     3361 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag
     3421 cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg
     3481 cgtggcagca tcaggggaaa actttattta tcagccggaa aacctaccgg attgatggta
     3541 gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc
     3601 ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
     3661 ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc
     3721 cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
     3781 cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
     3841 gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
     3901 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
     3961 cgtcagtatc ggcggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt
     4021 gtcaaaaata atagggggga tccactagtt ctagagcggc cgccaccgcg gggagatcca
     4081 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4141 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4201 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4261 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcccggc
     4321 tgcctcgcgc gtttcggtga tgacggtgaa aacctcttga cacatgcagc tcccggagac
     4381 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     4441 gggtgttggc gggtgtcggg gcgcagccat gaggtcgact ctagtccccg cggtggcaga
     4501 tctggaaggt gctgaggtac gatgagaccc gcaccaggtg cagaccctgc gagtgtggcg
     4561 gtaaacatat taggaaccag cctgtgatgc tggatgtgac cgaggagctg aggcccgatc
     4621 acttggtgct ggcctgcacc cgcgctgagt ttggctctag cgatgaagat acagattgag
     4681 gtactgaaat gtgtgggcgt ggcttaaggg tgggaaagaa tatataaggt gggggtctta
     4741 tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagcaccaac tcgtttgatg
     4801 gaagcattgt gagctcatat ttgacaacgc gcatgccccc atgggccggg gtgcgtcaga
     4861 atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc cgcaaactct actaccttga
     4921 cctacgagac cgtgtctgga acgccgttgg agactgcagc ctccgccgcc gcttcagccg
     4981 ctgcagccac cgcccgcggg attgtgactg actttgcttt cctgagcccg cttgcaagca
     5041 gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac ggctcttttg gcacaattgg
     5101 attctttgac ccgggaactt aatgtcgttt ctcagcagct gttggatctg cgccagcagg
     5161 tttctgccct gaaggcttcc tcccctccca atgcggttta aaacataaat aaaaaaccag
     5221 actctgtttg gatttggatc aagcaagtgt cttgctgtct ttatttaggg gttttgcgcg
     5281 cgcggtaggc ccgggaccag cggtctcggt cgttgagggt cctgtgtatt ttttccagga
     5341 cgtggtaaag gtgactctgg atgttcagat acatgggcat aagcccgtct ctggggtgga
     5401 ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt gtagatgatc cagtcgtagc
     5461 aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag caagctgatt gccaggggca
     5521 ggcccttggt gtaagtgttt acaaagcggt taagctggga tgggtgcata cgtggggata
     5581 tgagatgcat cttggactgt atttttaggt tggctatgtt cccagccata tccctccggg
     5641 gattcatgtt gtgcagaacc accagcacag tgtatccggt gcacttggga aatttgtcat
     5701 gtagcttaga aggaaatgcg tggaagaact tggagacgcc cttgtgacct ccaagatttt
     5761 ccatgcattc gtccataatg atggcaatgg gcccacgggc ggcggcctgg gcgaagatat
     5821 ttctgggatc actaacgtca tagttgtgtt ccaggatgag atcgtcatag gccattttta
     5881 caaagcgcgg gcggagggtg ccagactgcg gtataatggt tccatccggc ccaggggcgt
     5941 agttaccctc acagatttgc atttcccacg ctttgagttc agatgggggg atcatgtcta
     6001 cctgcggggc gatgaagaaa acggtttccg gggtagggga gatcagctgg gaagaaagca
     6061 ggttcctgag cagctgcgac ttaccgcagc cggtgggccc gtaaatcaca cctattaccg
     6121 ggtgcaactg gtagttaaga gagctgcagc tgccgtcatc cctgagcagg ggggccactt
     6181 cgttaagcat gtccctgact cgcatgtttt ccctgaccaa atccgccaga aggcgctcgc
     6241 cgcccagcga tagcagttct tgcaaggaag caaagttttt caacggtttg agaccgtccg
     6301 ccgtaggcat gcttttgagc gtttgaccaa gcagttccag gcggtcccac agctcggtca
     6361 cctgctctac ggcatctcga tccagcatat ctcctcgttt cgcgggttgg ggcggctttc
     6421 gctgtacggc agtagtcggt gctcgtccag acgggccagg gtcatgtctt tccacgggcg
     6481 cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg tgcgctccgg gctgcgcgct
     6541 ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag cgctgccggt cttcgccctg
     6601 cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc agcccctccg cggcgtggcc
     6661 cttggcgcgc agcttgccct tggaggaggc gccgcacgag gggcagtgca gacttttgag
     6721 ggcgtagagc ttgggcgcga gaaataccga ttccggggag taggcatccg cgccgcaggc
     6781 cccgcagacg gtctcgcatt ccacgagcca ggtgagctct ggccgttcgg ggtcaaaaac
     6841 caggtttccc ccatgctttt tgatgcgttt cttacctctg gtttccatga gccggtgtcc
     6901 acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca gacttgagag gcctgtcctc
     6961 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     7021 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     7081 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     7141 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     7201 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     7261 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     7321 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     7381 acgaccatca gggacagctt caaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7861 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8101 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8161 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8341 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8521 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8821 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9061 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9181 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9241 aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//