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pCMV-GLuc 2载体质粒基本信息
pCMV-GLuc 2质粒图谱载体图谱和pCMV-GLuc 2载体序列质粒序列多克隆位点信息
pCMV-GLuc 2质粒载体简介
pCMV-GLuc 2质粒序列载体序列
LOCUS pCMV-GLuc 2 5802 bp ds-DNA circular SYN 03-AUG-2013
DEFINITION Control vector with a strong constitutive CMV promoter for
expressing secreted Gaussia luciferase in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-GLuc 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5802)
AUTHORS New England Biolabs
TITLE Direct Submission
JOURNAL Exported Wednesday, February 26, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5802
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_signal 914..923
/note="Kozak sequence"
CDS 920..1477
/codon_start=1
/product="secreted Gaussia luciferase"
/note="hGLuc"
/note="human codon-optimized"
/translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
RCATFASKIQGQVDKIKGAGGD"
polyA_signal 1486..1534
/note="synthetic polyadenylation signal"
rep_origin 1668..2096
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2110..2440
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2291..2426
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2507..3301
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3475..3596
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4047..4635)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4806..5666)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5667..5771)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccagccacca tgggagtcaa agttctgttt gccctgatct gcatcgctgt
961 ggccgaggcc aagcccaccg agaacaacga agacttcaac atcgtggccg tggccagcaa
1021 cttcgcgacc acggatctcg atgctgaccg cgggaagttg cccggcaaga agctgccgct
1081 ggaggtgctc aaagagatgg aagccaatgc ccggaaagct ggctgcacca ggggctgtct
1141 gatctgcctg tcccacatca agtgcacgcc caagatgaag aagttcatcc caggacgctg
1201 ccacacctac gaaggcgaca aagagtccgc acagggcggc ataggcgagg cgatcgtcga
1261 cattcctgag attcctgggt tcaaggactt ggagcccatg gagcagttca tcgcacaggt
1321 cgatctgtgt gtggactgca caactggctg cctcaaaggg cttgccaacg tgcagtgttc
1381 tgacctgctc aagaagtggc tgccgcaacg ctgtgcgacc tttgccagca agatccaggg
1441 ccaggtggac aagatcaagg gggccggtgg tgactaagcg gccgcaataa aatatcttta
1501 ttttcattac atctgtgtgt tggttttttg tgtgtctaga aataattctt actgtcatgc
1561 caagtaagat gcttttctgt gctgcaatag caggcatgct ggggatgcgg tgggctctat
1621 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
1681 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
1741 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
1801 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca
1861 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
1921 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
1981 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg
2041 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
2101 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag
2161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
2221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
2281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
2341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
2401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
2461 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
2521 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
2581 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
2641 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
2701 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
2761 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
2821 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
2881 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
2941 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
3001 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
3061 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
3121 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
3181 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
3241 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
3301 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
3361 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
3421 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
3481 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3541 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
3601 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
3661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
3721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
3781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
3841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
3901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
3961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
4261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
4501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
4621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
4681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
4741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
4801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
4861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
4921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
4981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
5041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
5101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
5161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
5221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
5281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
5341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
5401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
5461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
5521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
5581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
5641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
5701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
5761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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